Genomic streamlining of seagrass-associatedColletotrichumsp. may be related to its adaptation to a marine monocot host DOI Creative Commons
Cassandra L. Ettinger, Jonathan A. Eisen, Jason Stajich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Colletotrichum spp. have a complicated history of association with land plants. Perhaps most well-known as plant pathogens for the devastating effect they can on agricultural crops, some been reported beneficial endophytes. However, there only handful reports isolated from aquatic hosts and their ecological role in marine ecosystem is underexplored. To address this, we present draft genome annotation sp. CLE4, previously rhizome tissue seagrass Zostera marina . This (48.03 Mbp length) highly complete (BUSCO ascomycota: 98.8%) encodes 12,015 genes, which 5.7% are carbohydrate-active enzymes (CAZymes) 12.6% predicted secreted proteins. Phylogenetic placement puts CLE4 within C. acutatum complex, closely related to godetiae We found 8.69% smaller size, 21.90% gene count, absence 591 conserved families relative other members suggesting streamlined possibly linked its specialized niche ecosystem. Machine learning analyses using CATAStrophy CAZyme domains predict this isolate be hemibiotroph, such that it has biotrophic phase where kept alive during optimal environmental conditions followed by necrotrophic fungi actively serves pathogen. While future work still needed definitively tease apart lifestyle strategy study provides foundational insight high-quality genomic resource starting understand evolutionary trajectory adaptations marine-plant associated fungi.

Language: Английский

Cytochrome P450 monooxygenase systems: diversity and plasticity for adaptive stress response DOI
Innokenty M. Mokhosoev, Dmitry V. Astakhov, Alexander A. Terentiev

et al.

Progress in Biophysics and Molecular Biology, Journal Year: 2024, Volume and Issue: 193, P. 19 - 34

Published: Sept. 6, 2024

Language: Английский

Citations

4
Long-term survival of asexual Zymoseptoria tritici spores in the environment DOI Creative Commons
William T. Kay, Paul O’Neill, Sarah J. Gurr

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 19, 2024

Abstract Background The fungal phytopathogen Zymoseptoria tritici , causal agent of the economically damaging Septoria blotch wheat, is different from most foliar pathogens in that its germination occurs slowly and apparently randomly after arrival on leaf surface followed by a potentially prolonged period epiphytic growth even reproduction, during which no feeding structures are formed fungus. Thus, understanding cues for mechanisms underpin survival low-nutrient environments could provide key new avenues disease control. Results In this work, we examine survival, culturability virulence spores following transfer high nutrient environment to water. We find sub-population Z. can survive remain virulent at least 7 weeks water alone, time multicellular split single cells. fungus relies heavily stored lipids; however, if cell suspensions dried, cells without lipid utilisation. Changes gene expression first hours suspension reflect adaptation stress, while longer term starvation (7 days) induces changes particularly primary metabolism cytochrome P450 (CYP) expression. Importantly, also found equally or better able soil as water, rain-splash occurring 49 days inoculation wheat seedlings growing inoculated cause disease. Conclusions blastospores long periods, spanning intercrop UK winter wheat. They rely internal stores, with external nutrition, although large proportion do not such an extended period, those grown rich media. has exceptional strategies, likely be important population genetics developing novel routes

Language: Английский

Citations

1
Hybrid de novo assembly of the genome of Colletotrichum acutatum sensu stricto isolate COL14 from olive fruit in Central Italy DOI
Silvia Turco, Federico Brugneti, Chiara Fiorenzani

et al.

Journal of Plant Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

Language: Английский

Citations

0
Genomic streamlining of seagrass-associatedColletotrichumsp. may be related to its adaptation to a marine monocot host DOI Creative Commons
Cassandra L. Ettinger, Jonathan A. Eisen, Jason Stajich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Colletotrichum spp. have a complicated history of association with land plants. Perhaps most well-known as plant pathogens for the devastating effect they can on agricultural crops, some been reported beneficial endophytes. However, there only handful reports isolated from aquatic hosts and their ecological role in marine ecosystem is underexplored. To address this, we present draft genome annotation sp. CLE4, previously rhizome tissue seagrass Zostera marina . This (48.03 Mbp length) highly complete (BUSCO ascomycota: 98.8%) encodes 12,015 genes, which 5.7% are carbohydrate-active enzymes (CAZymes) 12.6% predicted secreted proteins. Phylogenetic placement puts CLE4 within C. acutatum complex, closely related to godetiae We found 8.69% smaller size, 21.90% gene count, absence 591 conserved families relative other members suggesting streamlined possibly linked its specialized niche ecosystem. Machine learning analyses using CATAStrophy CAZyme domains predict this isolate be hemibiotroph, such that it has biotrophic phase where kept alive during optimal environmental conditions followed by necrotrophic fungi actively serves pathogen. While future work still needed definitively tease apart lifestyle strategy study provides foundational insight high-quality genomic resource starting understand evolutionary trajectory adaptations marine-plant associated fungi.

Language: Английский

Citations

0