Genetic Characterization of SWEET Genes in Coconut Palm DOI Creative Commons

Jiepeng Chen,

Weimin Zeng,

Jiali Mao

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 686 - 686

Published: Feb. 23, 2025

Sugar-Will-Eventually-be-Exported Transporters (SWEETs) play a crucial role in sugar transport plants, mediating both plant development and stress responses. Despite their importance, there has been limited research characterizing the functional characteristics of CnSWEET genes coconut (Cocos nucifera). In this study, we conducted systematic analysis SWEET using bioinformatics, subcellular localization studies, silico promoter analysis, assays with yeast mutants. A total 16 were identified grouped into four clades. Clade I contained highest number (eight), derived from pairs duplicated genomic segments. contrast, other clades had fewer (one to four) compared those Arabidopsis species Arecaceae family. An extensive gene expansion MSCanX indicated significant divergence patterns, between within monocots dicots, as well among closely related same Notable variations conserved protein motifs transmembrane helices (TMHs) detected clades, affecting proteins. Specifically, seven TMHs associated proteins located cell membrane, while CnSWEET2A, which five TMHs, was found membrane cytosol. Promoter revealed that some endosperm or seed specific expression, consistent expression patterns observed transcriptome data. Functional genes, transcript sequences supported by data, mutant complementation assays. This demonstrated diverse activities for sucrose, fructose, glucose, galactose, mannose. Our findings provide valuable insights diversity coconuts potential roles responses development.

Language: Английский

Genetic Characterization of SWEET Genes in Coconut Palm DOI Creative Commons

Jiepeng Chen,

Weimin Zeng,

Jiali Mao

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 686 - 686

Published: Feb. 23, 2025

Sugar-Will-Eventually-be-Exported Transporters (SWEETs) play a crucial role in sugar transport plants, mediating both plant development and stress responses. Despite their importance, there has been limited research characterizing the functional characteristics of CnSWEET genes coconut (Cocos nucifera). In this study, we conducted systematic analysis SWEET using bioinformatics, subcellular localization studies, silico promoter analysis, assays with yeast mutants. A total 16 were identified grouped into four clades. Clade I contained highest number (eight), derived from pairs duplicated genomic segments. contrast, other clades had fewer (one to four) compared those Arabidopsis species Arecaceae family. An extensive gene expansion MSCanX indicated significant divergence patterns, between within monocots dicots, as well among closely related same Notable variations conserved protein motifs transmembrane helices (TMHs) detected clades, affecting proteins. Specifically, seven TMHs associated proteins located cell membrane, while CnSWEET2A, which five TMHs, was found membrane cytosol. Promoter revealed that some endosperm or seed specific expression, consistent expression patterns observed transcriptome data. Functional genes, transcript sequences supported by data, mutant complementation assays. This demonstrated diverse activities for sucrose, fructose, glucose, galactose, mannose. Our findings provide valuable insights diversity coconuts potential roles responses development.

Language: Английский

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