Polyploidization-driven transcriptomic dynamics in Medicago sativa neotetraploids: mRNA, smRNA and allele-specific gene expression

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 25, 2025

Whole genome duplication (WGD) is a powerful evolutionary mechanism in plants. Autopolyploids have been comparatively less studied than allopolyploids, with sexual autopolyploidization receiving even attention. In this work, we the transcriptomes of neotetraploids (2n = 4x 32) obtained by crossing two diploid 2x 16) plants Medicago sativa that produce significant percentage either 2n eggs or pollen. Diploid progeny from same cross allowed us to separate transcriptional outcomes hybridization those WGD. This material can help elucidate events at base domestication cultivated alfalfa, world's most important leguminous forage. Three and three parental were used for study. The RNA-seq data revealed WGD did not dramatically affect transcription leaf protein-coding genes. genotypes contribute equally transcriptomes, genome-wide expression level dominance male parent was observed. A large majority genes whose changed due presented increased expression, indicating state requires upregulation approximately 2.66% Overall, estimated 3.63% transcriptionally affected may phenotypic novelty neotetraploid Pathway analysis suggested could secondary metabolite biosynthesis, which turn influence forage quality. We found four times as many factor among polyploidization-affected only hybridization. Several these belong classes involved stress response. Small very few miRNAs significantly associated WGD, but they target several hundred genes, their role response be relevant. Integrated network led identification putative miRNA: mRNA interactions potentially transcriptome reprogramming. Allele-specific indicated parent-of-origin bias outcome parentally biased RNA editing source variation neopolyploids.

Language: Английский

Transcriptional Profiling Analysis Providing Insights into the Harsh Environments Tolerance Mechanisms of Krascheninnikovia arborescens

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11891 - 11891

Published: Nov. 5, 2024

, an endemic shrub in China, thrives desertification-prone environments due to its robust biomass, hairy leaves, and extensive root system. It is vital for ecological restoration serves as a valuable forage plant. This study explored the molecular mechanisms underlying

Language: Английский