Identification of the bHLH Transcription Factor Family in Orah Mandarin and the Response of CrbHLH46 to Low-Temperature Stress

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 882 - 882

Published: March 12, 2025

As the second largest family of transcription factors (TFs) in plants, basic helix–loop–helices (bHLHs) play key roles regulating plant growth and development responding to environmental stress. fastest growing Citrus variety China recent years, Orah mandarin has vital economic nutritional value. Although a comprehensive genome-wide analysis bHLH TF been performed many systematic study genes this not carried out mandarin. In study, 114 TFs were identified via classified into 27 subfamilies according evolutionary tree. The gene expression profile revealed that five significantly upregulated at 12 h 24 after low-temperature stress treatment. addition, soluble sugars, proteins, proline contents increased with increasing stress, which promoted CrbHLH46 gene, thus mediating interconversion pathway pentose glucose improve cold tolerance results help explore characteristics functions CrbHLH provide basis for further research on resistance

Language: Английский

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Investigating TIFY Genes for Salt Stress Adaptation in Quinoa (Chenopodium quinoa Willd.): A Genome-Wide Approach

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Journal Year: 2025, Volume and Issue: 18(1), P. 213 - 228

Published: March 26, 2025

TIFY ailesi, TIFY, Jas ve GATA motiflerini içeren bitkilerin savunma mekanizmalarında stres faktörlerine karşı verdikleri yanıtta önemli rol oynayan bir gen ailesidir. ailesi birçok bitki türünde araştırılmış olmasına rağmen, kinoada henüz incelenmemiştir. Bu çalışmada, 16 Cq-TIFY geni tanımlanmış, bu genler Cq-TIFY-1’den Cq-TIFY-16’ya kadar numaralandırılarak yapısal işlevsel özellikleri karakterize edilmiştir. Tanımlanan proteinlerinin moleküler ağırlıkları 19,99 ile 48,59 kDa, amino asit sayıları 189 450, teorik izoelektrik noktaları ise 4,84 10,1 arasında değişmektedir. Filogenetik analiz sonuçlarına göre, genlerinin üç sınıfa ayrıldığı belirlenmiştir. Gen ailesinin farklı sınıflarındaki üyelerin yapılarının genellikle benzer olduğu Kinoa’da yedi segmental duplikasyon geçirmiş tanımlanmış olup, Ka/Ks analizi genlerin evrimsel süreçte arındırıcı (negatif) seçilime maruz kaldığını göstermiştir. Chenopodium quinoa, Arabidopsis thaliana Spinacia oleracea türleri arasındaki sinteni analizi, genleri açısından ilişki olduğunu ortaya koymuştur. Promotör sonucunda, genlerinde strese duyarlı hormonla ilişkili cis-elementlerin varlığı çıkarılmıştır. Araştırmada, RNA-seq verileri, tuz koşulları altında kök sürgün dokularında ifade modellerini incelemek için kullanılmıştır. Genlerin stresi altındaki profili köklerde sürgünlerde dokuya özgü olarak farklılık göstermiş ifadelerinde anlamlı artış sonuç, toleransı oynayabileceğini düşündürmüştür. çalışma, kinoadaki ailesine dair bilgimizi artırmakta klasik ıslah veya genetik mühendisliği yoluyla toleransını artırmaya yönelik temel oluşturmaktadır.

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Genome-wide identification of carotenoid cleavage oxygenase genes in Orah mandarin and the mechanism by which CrCCD4b1 affects peel color

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 338, P. 113652 - 113652

Published: Sept. 18, 2024

Language: Английский

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JAZ proteins: Key regulators of plant growth and stress response

The Crop Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Language: Английский

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Genome-wide identification and expression analysis of the SOD gene family under biotic and abiotic stresses in sweet orange

Plant Biotechnology Reports, Journal Year: 2024, Volume and Issue: 18(4), P. 535 - 549

Published: July 10, 2024

Language: Английский

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A new pathway model of the response of Hsf gene family members to abiotic and biotic stresses in sweet orange revealed by genome-wide identification and expression profile analysis

South African Journal of Botany, Journal Year: 2024, Volume and Issue: 174, P. 23 - 39

Published: Sept. 6, 2024

Language: Английский

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Transcriptomic Profiling Analyses Revealed Candidate Genes Under Freezing Stress in Siberian Apricot (Prunus sibirica)

Forests, Journal Year: 2024, Volume and Issue: 15(11), P. 1870 - 1870

Published: Oct. 24, 2024

Siberian apricot (Prunus sibirica) is an important ecological and commercial woody plant that negatively affected by spring frosts. However, the mechanisms control gene expression in adaptation to freezing remain largely unknown. In this work, we investigated physiological, molecular, phenotypic characteristics of pistils two P. sibirica clones differ their ability withstand stress. A total 14,717 unigenes categorized into 38 functional groups were identified. Additionally, included 3931 up-regulated 2070 down-regulated differentially expressed genes (DEGs). Many DEGs are related Ca2+ MAPK signaling, carbohydrate biosynthesis metabolism, hormone signal transduction, amino acids, photosynthesis. The metabolism carbohydrates, lipids, secondary metabolites, terpenoid transcriptome modifications most significantly altered Real-time quantitative PCR (RT-qPCR) was used verify precision RNA-seq data. PsbHLH18, PsMYB4, PsMYB44, PsPOD1, PsCDPK5 may play roles tolerance floral organ. This study provides a foundation for further studies on complex stress response sibirica.

Language: Английский

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Expression Profiling Analysis of the SWEET Gene Family in In Vitro Pitaya Under Low-Temperature Stress and Study of Its Cold Resistance Mechanism

Plants, Journal Year: 2024, Volume and Issue: 13(21), P. 3092 - 3092

Published: Nov. 2, 2024

Pitaya (Hylocereus undatus) fruit is an attractive, nutrient-rich tropical with commercial value. However, low-temperature stress severely affects the yield and quality of pitaya. The relevant mechanisms involved in response pitaya to remain unclear. To study whether SWEET gene family mediates H. undatus related mechanisms, we performed genome-wide identification pitaya, used ‘Baiyulong’ tissue-cultured plantlets as material present study. We identified 28 members from genome divided these into four groups. Members this presented some differences sequences introns exons, but structure, especially motifs, relatively conserved characteristics. promoter regions most HuSWEETs have multiple stress- or hormone-related cis-elements. Three duplicated pairs were identified, including one tandem duplication two fragment pairs. results revealed that genes may regulate transport distribution soluble sugars plants; indirectly enzyme activities CAT, POD, T-SOD through its expression products; are play vital roles process. After ABA MeJA treatment, changed significantly, cold was also alleviated. This elucidated molecular mechanism physiological changes sugar metabolism when it experiences provided a theoretical basis for cold-resistant variety breeding.

Language: Английский

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Integrated transcriptome and metabolome analyses reveal the mechanism by which bagging treatment affects peel reddening in Orah mandarin

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 221, P. 113336 - 113336

Published: Nov. 26, 2024

Language: Английский

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