Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2521 - 2521
Published: Sept. 8, 2024
Grain weight and grain shape are important traits that determine rice yield quality. Mining more quantitative trait loci (QTLs) control will help to further improve the molecular regulatory network of development provide gene resources for high-yield high-quality varieties. In present study, a QTL length (GL) width (GW),
Language: Английский
Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16
Published: Feb. 25, 2025
Dirigent (DIR) proteins are key regulators of lignin and lignan biosynthesis play critical roles in plant hormone responses, abiotic stress tolerance, growth development. This study identified characterized 47 PeDIR genes Moso bamboo, classifying them into three groups. Phylogenetic comparative analyses revealed strong evolutionary conservation, with the bamboo being most closely related to those rice maize. DIR within each subfamily exhibited high conservation motif composition, domain structure, 3D configuration. Subcellular localization protein interaction studies further elucidated gene functions. Specifically, PeDIR02 primarily localized cell membrane was shown be unable form homodimers yeast two-hybrid (Y2H) assays. Transcriptome expression involvement rapid shoot growth, indicating wall modification. qRT-PCR data also demonstrated responsiveness these hormones stresses, such as drought salinity. constructed first comprehensive regulatory network between transcription factors (TFs) genes, identifying ERF, DOF, MYB TFs synergistic expression.
Language: Английский
Citations
0
Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110074 - 110074
Published: May 1, 2025
Language: Английский
Citations
0
Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 604 - 604
Published: Feb. 28, 2025
The DIR gene family, which encodes Dirigent proteins, plays a crucial role in plant development and stress responses. However, the functions mechanisms of this family maize remained underexplored. This study identified ZmDIR11, member protein explored its drought tolerance. ZmDIR11 shared conserved regions with homologous proteins wheat, rice, Arabidopsis. RT-qPCR analysis revealed that expression is upregulated leaves under PEG stress, while subcellular localization confirmed presence endoplasmic reticulum. Virus-induced silencing (VIGS) EMS mutagenesis demonstrated or mutation significantly reduces tolerance seedlings, indicating positive regulatory role. Silencing led to decreased growth parameters (plant height, root length, fresh weight, dry chlorophyll content) alongside reduced antioxidant capacity, as evidenced by increased levels MDA ROS activities SOD, CAT, POD. Furthermore, impaired synthesis ABA zeatin, downregulating key genes (ZmABA1, ZmNCED3, ZmSnRK2.6) zeatin (ZmIPT4, ZmCKO5, ZmCKO4b) pathways. Drought-responsive (ZmRD20, ZmRD22, ZmDREB2A) lignan biosynthesis (Zm4CL, ZmC3H, ZmCAD1) are also downregulated mutants. In conclusion, enhances regulating synthesis, metabolism. provides new insights into offers potential genetic resource for breeding drought-resistant varieties.
Language: Английский
Citations
0
Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2521 - 2521
Published: Sept. 8, 2024
Grain weight and grain shape are important traits that determine rice yield quality. Mining more quantitative trait loci (QTLs) control will help to further improve the molecular regulatory network of development provide gene resources for high-yield high-quality varieties. In present study, a QTL length (GL) width (GW),
Language: Английский
Citations
1