The PopbZIP2‐PopMYB4 regulatory module enhances disease resistance in poplars by modulating proanthocyanidin accumulation
Shuxian Tan,
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Sisi Chen,
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Haoyu Zhang
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et al.
New Phytologist,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 13, 2025
Summary
Anthracnose,
caused
by
Colletotrichum
gloeosporioides
,
is
a
significant
fungal
disease
that
affects
poplar
trees
globally,
leading
to
reduced
yields
and
substantial
economic
losses.
Proanthocyanidins
(PAs)
play
key
role
in
resistance
pathogens;
however,
the
mechanisms
which
PAs
mediate
anthracnose
remain
poorly
understood.
In
this
study,
we
identified
PopbZIP2
transcription
factor‐encoding
gene
was
initially
expressed
infected
leaves
subsequently
uninfected
response
C.
infection.
As
transcriptional
activator,
can
bind
promoters
of
target
genes
PopGRF3
PopAPA1
increasing
proanthocyanidin
levels
cells
enhance
defense
against
pathogens.
It
noteworthy
protein
directly
inhibit
pathogen
growth.
We
further
demonstrated
PopMYB4
interact
with
PopbZIP2,
reducing
its
promoter
binding
activity
thereby
inhibiting
expression
PopAPA1.
Overexpression
led
sensitivity
gloeosporiodes
.
Under
normal
conditions,
soluble
insoluble
contents
transgenic
plants
were
significantly
lower
compared
control.
The
dual
regulation
immune
responses
PopMYB4‐PopbZIP2
module
unveils
novel
regulatory
mechanism
Populus
enhancing
our
understanding
complex
networks
governing
responses.
Language: Английский
Expanding the functional landscape of microbial entomopathogens in agriculture beyond pest management
Nikita Saha,
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Anwesha Sharma,
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Popy Bora
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et al.
Folia Microbiologica,
Journal Year:
2025,
Volume and Issue:
70(2), P. 343 - 357
Published: March 5, 2025
Language: Английский
Discovery of a novel fungal Tc toxin complex and a functional myco- serpin via a unique two-by-two comparative genomics pipeline
Zack Saud,
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Yujuan Luo,
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Martyn J. Wood
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et al.
Research Square (Research Square),
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 21, 2025
Abstract
Background
Fungi
have
been
a
rich
source
of
pharmaceuticals
such
as
antibiotics,
immunosuppressants,
and
cholesterol-lowering
drugs;
however,
their
therapeutic
potential
remains
largely
untapped
due
to
difficulties
in
culturing
elucidating
the
genetic
basis
beneficial
traits.
contain
'cryptic'
genes
that
are
expressed
under
certain,
often
obscure,
growth
conditions
can
produce
complex
compounds
difficult
synthesize
economically.
Developments
genome
sequencing
DNA-synthesis
technologies
offer
new
opportunities
using
biotechnological
techniques,
accurately
identifying
useful
novel
genes,
prerequisite
for
approaches,
challenging.
Results
We
present
‘two-by-two’
comparative
genomics
pipeline
comprehensive
gene
analysis
selected
fungal
groups,
enabling
more
confident
identification
unique
across
analyzed
species.
The
approach
compares
sets
from
two
strains
same
species
with
those
different
or
families
within
order.
Self-clustering
orthologs
provide
higher
confidence
species-specific
proteins
help
reduce
noise
low-quality
assemblies
prediction
errors.
validated
our
method
on
well-studied
group
fungi,
discovering
first
functional
myco-serpin
an
undescribed
Tc
toxin
complex.
Using
knockout
approach,
we
implicated
both
proteins’
roles
insect
host
infection
process
this
entomopathogenic
Conclusions
Elucidating
underlying
traits
fungi
presents
significant
challenges,
relatively
aspects
lifestyles.
Our
two-by-two
offers
broad
applications
mining
bioprospecting
exemplified
study
by
discovery
myco-serpin.
identified
high
sequence
identity
serpin
other
pathogenic
strains,
including
known
infect
humans.
Furthermore,
be
adapted
organisms
architectures
similar
fungi.
Language: Английский