Discovery of a novel fungal Tc toxin complex and a functional myco- serpin via a unique two-by-two comparative genomics pipeline DOI Creative Commons
Zack Saud,

Yujuan Luo,

Martyn J. Wood

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Abstract Background Fungi have been a rich source of pharmaceuticals such as antibiotics, immunosuppressants, and cholesterol-lowering drugs; however, their therapeutic potential remains largely untapped due to difficulties in culturing elucidating the genetic basis beneficial traits. contain 'cryptic' genes that are expressed under certain, often obscure, growth conditions can produce complex compounds difficult synthesize economically. Developments genome sequencing DNA-synthesis technologies offer new opportunities using biotechnological techniques, accurately identifying useful novel genes, prerequisite for approaches, challenging. Results We present ‘two-by-two’ comparative genomics pipeline comprehensive gene analysis selected fungal groups, enabling more confident identification unique across analyzed species. The approach compares sets from two strains same species with those different or families within order. Self-clustering orthologs provide higher confidence species-specific proteins help reduce noise low-quality assemblies prediction errors. validated our method on well-studied group fungi, discovering first functional myco-serpin an undescribed Tc toxin complex. Using knockout approach, we implicated both proteins’ roles insect host infection process this entomopathogenic Conclusions Elucidating underlying traits fungi presents significant challenges, relatively aspects lifestyles. Our two-by-two offers broad applications mining bioprospecting exemplified study by discovery myco-serpin. identified high sequence identity serpin other pathogenic strains, including known infect humans. Furthermore, be adapted organisms architectures similar fungi.

Language: Английский

The PopbZIP2PopMYB4 regulatory module enhances disease resistance in poplars by modulating proanthocyanidin accumulation DOI Open Access

Shuxian Tan,

Sisi Chen, Haoyu Zhang

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Summary Anthracnose, caused by Colletotrichum gloeosporioides , is a significant fungal disease that affects poplar trees globally, leading to reduced yields and substantial economic losses. Proanthocyanidins (PAs) play key role in resistance pathogens; however, the mechanisms which PAs mediate anthracnose remain poorly understood. In this study, we identified PopbZIP2 transcription factor‐encoding gene was initially expressed infected leaves subsequently uninfected response C. infection. As transcriptional activator, can bind promoters of target genes PopGRF3 PopAPA1 increasing proanthocyanidin levels cells enhance defense against pathogens. It noteworthy protein directly inhibit pathogen growth. We further demonstrated PopMYB4 interact with PopbZIP2, reducing its promoter binding activity thereby inhibiting expression PopAPA1. Overexpression led sensitivity gloeosporiodes . Under normal conditions, soluble insoluble contents transgenic plants were significantly lower compared control. The dual regulation immune responses PopMYB4‐PopbZIP2 module unveils novel regulatory mechanism Populus enhancing our understanding complex networks governing responses.

Language: Английский

Citations

0

Expanding the functional landscape of microbial entomopathogens in agriculture beyond pest management DOI

Nikita Saha,

Anwesha Sharma,

Popy Bora

et al.

Folia Microbiologica, Journal Year: 2025, Volume and Issue: 70(2), P. 343 - 357

Published: March 5, 2025

Language: Английский

Citations

0

Discovery of a novel fungal Tc toxin complex and a functional myco- serpin via a unique two-by-two comparative genomics pipeline DOI Creative Commons
Zack Saud,

Yujuan Luo,

Martyn J. Wood

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Abstract Background Fungi have been a rich source of pharmaceuticals such as antibiotics, immunosuppressants, and cholesterol-lowering drugs; however, their therapeutic potential remains largely untapped due to difficulties in culturing elucidating the genetic basis beneficial traits. contain 'cryptic' genes that are expressed under certain, often obscure, growth conditions can produce complex compounds difficult synthesize economically. Developments genome sequencing DNA-synthesis technologies offer new opportunities using biotechnological techniques, accurately identifying useful novel genes, prerequisite for approaches, challenging. Results We present ‘two-by-two’ comparative genomics pipeline comprehensive gene analysis selected fungal groups, enabling more confident identification unique across analyzed species. The approach compares sets from two strains same species with those different or families within order. Self-clustering orthologs provide higher confidence species-specific proteins help reduce noise low-quality assemblies prediction errors. validated our method on well-studied group fungi, discovering first functional myco-serpin an undescribed Tc toxin complex. Using knockout approach, we implicated both proteins’ roles insect host infection process this entomopathogenic Conclusions Elucidating underlying traits fungi presents significant challenges, relatively aspects lifestyles. Our two-by-two offers broad applications mining bioprospecting exemplified study by discovery myco-serpin. identified high sequence identity serpin other pathogenic strains, including known infect humans. Furthermore, be adapted organisms architectures similar fungi.

Language: Английский

Citations

0