Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi DOI Creative Commons
Changming Liu, Yang Liu, Xinyue Zhang

et al.

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 697 - 697

Published: April 25, 2025

Long non-coding RNAs (lncRNAs) have been defined as transcripts exceeding 200 nucleotides, which play essential roles during transcriptional and post-transcriptional regulation in multiple biological processes. Understanding R. delavayi lncRNAs is of great significance it profoundly influenced by heat stress. In this research, a total 1145 differentially expressed (DE-lncRNAs) 9447 genes (DEGs) (log2|FC| > 1, p < 0.05) were characterized between heat-stress-treated groups control groups. Further analysis showed that 229 DE-lncRNAs (144 upregulated 85 downregulated) commonly distributed two comparisons (CK_vs._H3 CK_vs._H6). We further investigated the cis- trans-acting DE-lncRNAs, found 142 corresponded to 1565 cis-acting DEGs, 143 3417 DEGs. KEGG enrichment these target revealed DEGs DE-lncRNA primarily enriched five twelve pathways, respectively. Co-expression network common pathways 57 co-expression relationships 28 43 554 26 90 Six six their used candidate verify RNA-seq data using qRT-PCR. three (TrxG, PEPC, CCR) out selected for subsequent research. This study examined relationship screened can potentially provide an important theoretical basis experimental genetic improvement tolerance delavayi.

Language: Английский

Integrated Analysis of Long Non-Coding RNAs and mRNAs Reveals Key Trans-Target Genes Associated with Heat Stress Response in Rhododendron delavayi DOI Creative Commons
Changming Liu, Yang Liu, Xinyue Zhang

et al.

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 697 - 697

Published: April 25, 2025

Long non-coding RNAs (lncRNAs) have been defined as transcripts exceeding 200 nucleotides, which play essential roles during transcriptional and post-transcriptional regulation in multiple biological processes. Understanding R. delavayi lncRNAs is of great significance it profoundly influenced by heat stress. In this research, a total 1145 differentially expressed (DE-lncRNAs) 9447 genes (DEGs) (log2|FC| > 1, p < 0.05) were characterized between heat-stress-treated groups control groups. Further analysis showed that 229 DE-lncRNAs (144 upregulated 85 downregulated) commonly distributed two comparisons (CK_vs._H3 CK_vs._H6). We further investigated the cis- trans-acting DE-lncRNAs, found 142 corresponded to 1565 cis-acting DEGs, 143 3417 DEGs. KEGG enrichment these target revealed DEGs DE-lncRNA primarily enriched five twelve pathways, respectively. Co-expression network common pathways 57 co-expression relationships 28 43 554 26 90 Six six their used candidate verify RNA-seq data using qRT-PCR. three (TrxG, PEPC, CCR) out selected for subsequent research. This study examined relationship screened can potentially provide an important theoretical basis experimental genetic improvement tolerance delavayi.

Language: Английский

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