Effect of Kaempferia parviflora Supplementation in Semen Extenders on Post-Thaw Sperm Quality in Thai Native Bulls DOI Creative Commons

Thanapon Loetjettanarom,

Supakorn Authaida, Wuttigrai Boonkum

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 962 - 962

Published: March 27, 2025

Cryopreservation is a widely used technique for long-term semen storage; however, it can induce oxidative stress, compromising sperm quality. This study examines the effect of Kaempferia parviflora (KP) supplementation in extenders on post-thaw quality Thai native bulls. Fresh was collected and evaluated motility, viability, concentration prior to cryopreservation. Semen samples were allocated four treatment groups: 0 mg/mL (control), 0.5 mg/mL, 1 1.5 KP. Subsequently, frozen, then thawed, analyzed membrane integrity, stress. The results showed that with 1.0 KP significantly improved motility (46.29 ± 2.66%) viability (43.42 2.15%) compared control (40.77 2.76% 38.63 2.66%, respectively). Membrane integrity also enhanced (47.64 1.18% vs. 41.85 1.98% control), while stress levels reduced (MDA concentration: 2.33 0.29 µM/mL 2.73 0.33 control). However, highest (1.5 KP) negatively affected quality, (36.97 3.32%), (30.88 3.02%), (35.64 1.61%). These findings suggest optimal improving cryopreservation protocols

Language: Английский

Effect of Kaempferia parviflora Supplementation in Semen Extenders on Post-Thaw Sperm Quality in Thai Native Bulls DOI Creative Commons

Thanapon Loetjettanarom,

Supakorn Authaida, Wuttigrai Boonkum

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(7), P. 962 - 962

Published: March 27, 2025

Cryopreservation is a widely used technique for long-term semen storage; however, it can induce oxidative stress, compromising sperm quality. This study examines the effect of Kaempferia parviflora (KP) supplementation in extenders on post-thaw quality Thai native bulls. Fresh was collected and evaluated motility, viability, concentration prior to cryopreservation. Semen samples were allocated four treatment groups: 0 mg/mL (control), 0.5 mg/mL, 1 1.5 KP. Subsequently, frozen, then thawed, analyzed membrane integrity, stress. The results showed that with 1.0 KP significantly improved motility (46.29 ± 2.66%) viability (43.42 2.15%) compared control (40.77 2.76% 38.63 2.66%, respectively). Membrane integrity also enhanced (47.64 1.18% vs. 41.85 1.98% control), while stress levels reduced (MDA concentration: 2.33 0.29 µM/mL 2.73 0.33 control). However, highest (1.5 KP) negatively affected quality, (36.97 3.32%), (30.88 3.02%), (35.64 1.61%). These findings suggest optimal improving cryopreservation protocols

Language: Английский

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