Gold Nanoparticles Carrying Mannose-Binding Lectin and Inflammatory Cytokine Antibodies Improve Sepsis Survival by Modulating Immunity and Reducing Pathogens DOI
Jian Mao, Yujie Yan, Qimei Wu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Sepsis is a systemic inflammatory response syndrome caused by infection, and early management of both the infection excessive key to its treatment. In this study, we designed nanoformulation, termed AuNPs-Mixed, control bacterial modulate response. AuNPs-Mixed was prepared equimolarly combining four nanoparticle formulations, each consisting gold nanoparticles (AuNPs) conjugated separately with mannose-binding lectin (MBL) three different antibodies targeting pro-inflammatory cytokines: interleukin 6 (IL-6), 1β (IL-1β), tumor necrosis factor alpha (TNF-α). MBL facilitates targeted recognition pathogenic bacteria, while cytokine-specific aim reduce levels cytokines. The formulation administered septic mice for 72 h. results showed that, compared groups treated AuNPs alone, carrying (AuNPs-MBL), blank group, receiving treatment exhibited significantly lower loads in blood, liver, spleen, kidneys (p < 0.05), reduced cytokines, less organ damage, markedly higher survival rates 0.05). Fluorescence confocal microscopy confirmed that AuNPs-MBL preparation could bind Escherichia coli, primary infectious agent sepsis model, facilitating subsequent phagocytosis macrophages. acute toxicity no significant differences were observed body weight, complete blood cell counts, or histopathological analysis major organs over 7 days > conclusion, study demonstrates effectively reduces organs, lowers cytokine levels, mitigates improves showing evident mice.

Language: Английский

Gold Nanoparticles Carrying Mannose-Binding Lectin and Inflammatory Cytokine Antibodies Improve Sepsis Survival by Modulating Immunity and Reducing Pathogens DOI
Jian Mao, Yujie Yan, Qimei Wu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Sepsis is a systemic inflammatory response syndrome caused by infection, and early management of both the infection excessive key to its treatment. In this study, we designed nanoformulation, termed AuNPs-Mixed, control bacterial modulate response. AuNPs-Mixed was prepared equimolarly combining four nanoparticle formulations, each consisting gold nanoparticles (AuNPs) conjugated separately with mannose-binding lectin (MBL) three different antibodies targeting pro-inflammatory cytokines: interleukin 6 (IL-6), 1β (IL-1β), tumor necrosis factor alpha (TNF-α). MBL facilitates targeted recognition pathogenic bacteria, while cytokine-specific aim reduce levels cytokines. The formulation administered septic mice for 72 h. results showed that, compared groups treated AuNPs alone, carrying (AuNPs-MBL), blank group, receiving treatment exhibited significantly lower loads in blood, liver, spleen, kidneys (p < 0.05), reduced cytokines, less organ damage, markedly higher survival rates 0.05). Fluorescence confocal microscopy confirmed that AuNPs-MBL preparation could bind Escherichia coli, primary infectious agent sepsis model, facilitating subsequent phagocytosis macrophages. acute toxicity no significant differences were observed body weight, complete blood cell counts, or histopathological analysis major organs over 7 days > conclusion, study demonstrates effectively reduces organs, lowers cytokine levels, mitigates improves showing evident mice.

Language: Английский

Citations

0