Genome analysis of a coral-associated bacterial consortium highlights complementary hydrocarbon degradation ability and other beneficial mechanisms for the host

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: July 28, 2023

Abstract Here we report the oil degradation genetic potential of six oil-degrading bacteria (ODB), previously used as a bioremediation consortium, isolated from hydrocoral Millepora alcicornis and seawater. The strains were identified Halomonas sp. (LC_1), Cobetia (LC_6), Pseudoalteromonas shioyasakiensis (LC_2), Halopseudomonas aestusnigri (LC_3), Shewanella algae (LC_4), Brucella intermedia (LC_5). taxonomic identification differed that original paper when whole genome gene markers instead just 16S rRNA gene. Genes responsible for aromatic hydrocarbons n-alkanes found in all genomes, although different (and complementary) steps metabolic pathways unique to each strain. naphthalene toluene various strains. We annotated quinate genes LC_6, while LC_3 LC_5 presented biosurfactant rhamnolipid biosynthesis. also related beneficial mechanisms corals, such involved nitrogen DMSP metabolism, cobalamin biosynthesis antimicrobial compounds production. Our findings reinforce importance using bacterial consortia approaches single strains, due their complementary genomic arsenals. propose genome-based framework select ODB can provide additional benefits coral health.

Language: Английский

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A coral-associated actinobacterium mitigates coral bleaching under heat stress

Environmental Microbiome, Journal Year: 2023, Volume and Issue: 18(1)

Published: Nov. 23, 2023

The positive effects of exposing corals to microorganisms have been reported though how the benefits are conferred poorly understood. Here, we isolated an actinobacterial strain (SCSIO 13291) from Pocillopora damicornis with capabilities synthesize antioxidants, vitamins, and antibacterial antiviral compounds supported phenotypic and/or genomic evidence. Strain SCSIO 13291 was labeled 5 (and - 6)-carboxytetramethylrhodamine, succinimidyl ester cell suspension directly inoculated onto coral polyp tissues when nubbins were under thermal stress in a mesocosm experiment. We then visualized labelled bacterial cells analyzed physiological, transcriptome microbiome elucidate effect this on holobiont stress.Subsequent microscopic observations confirmed presence bacterium attached polyps. Addition reduced signs bleaching subjected heat stress. At same time, alterations gene expression, which involved reactive oxygen species light damage mitigation, attenuated apoptosis exocytosis addition metabolite utilization, observed host Symbiodiniaceae populations. In addition, associated community altered more stable ecological network for samples strain.Our results provide insights into putative probiotic that mitigate signs. This study suggests inoculation bacteria can potentially benefit through conferring metabolic activities or indirect mechanisms suppling additional nutrient sources.

Language: Английский

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Long-term integrated rice-crayfish culture disrupts the microbial communities in paddy soil

Aquaculture Reports, Journal Year: 2023, Volume and Issue: 29, P. 101515 - 101515

Published: Feb. 27, 2023

The integrated rice-crayfish culture (IRCC) mode has been developed for more than twenty years in China, but little information could be obtained on the microbial communities paddy soil under this mode. This study used 16SrRNA and ITS Illumina high-throughput sequencing to investigate communities, diversity, function different IRCC times (0, 2, 4, 6, 10 years). With increasing time, altered relative abundance of dominant taxa, showing a significant decrease Actinobacteria Chytridiomycota, with an increase Proteobacteria Firmicutes. species richness diversity bacteria/fungi 10-year were significantly lower other groups (P < 0.05). Bacterial functional potential prediction by PICRUSt2 phenotype BugBase showed that long-term reduced carbohydrate metabolism increased pathogenic anaerobic Saprotrophs pathotrophs dominated fungal higher abundances plant pathogens, parasites lichen pathogens found (2- 4-year) Microbial mainly influenced ammonium nitrogen, nitrate moisture content, total carbon. Long-term change functions, while reducing soil. A "resting period" is recommended ensure sustainable development ecological agriculture fields.

Language: Английский

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Host under epigenetic control: A novel perspective on the interaction between microorganisms and corals

BioEssays, Journal Year: 2021, Volume and Issue: 43(10)

Published: Aug. 31, 2021

Abstract Coral reefs have been challenged by the current rate and severity of environmental change that might outpace their ability to adapt survive. Current research focuses on understanding how microbial communities epigenetic changes separately affect phenotypes gene expression corals. Here, we provide hypothesis coral‐associated microorganisms may directly or indirectly coral's phenotypic response through modulation its epigenome. Homologs ankyrin‐repeat protein A internalin B, which cause histone modifications in humans, as well Rv1988 methyltransferase, DNA methyltransferases Rv2966c, Mhy1, Mhy2, Mhy3 found bacteria indicate there are potential host epigenome‐modifying proteins coral microbiome. With ideas presented here, suggest microbiome manipulation be a means alter epigenome, could aid efforts protect reefs. Also see video abstract here: https://youtu.be/CW9GbChjKM4

Language: Английский

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Antibiotics Alter Pocillopora Coral-Symbiodiniaceae-Bacteria Interactions and Cause Microbial Dysbiosis During Heat Stress

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 8

Published: Jan. 13, 2022

Symbioses between eukaryotes and their associated microbial communities are fundamental processes that affect organisms’ ecology evolution. A unique example of this is reef-building corals maintain symbiotic associations with dinoflagellate algae (Symbiodiniaceae) bacteria coral health through various mechanisms. However, little understood about how coral-associated holobiont heat tolerance. In study, we investigated these interactions in four Pocillopora colonies belonging to three cryptic species by subjecting fragments treatments antibiotics intended suppress the normal community, followed acute stress. Separate only or stress were conducted compare effects individual stressors on transcriptome responses microbiome shifts. Across all examined, combined treatment significantly altered caused major changes both Cladocopium algal symbiont gene expression. Individually, impaired protein translation activated DNA repair processes, while downregulation amino acid inorganic ion transport metabolism genes photosynthesis genes. Combined antibiotics-heat synergistic expression including enhanced oxidative response genes, programed cell death pathways proteolytic enzymes indicate an exacerbated following community suppression. Collectively, results provide further evidence Symbiodiniaceae engage highly coordinated metabolic crucial for health, homeostasis,

Language: Английский

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