Biocontrol potential of endophytic bacterium Bacillus altitudinis GS-16 against tea anthracnose caused by Colletotrichum gloeosporioides

PeerJ, Journal Year: 2024, Volume and Issue: 12, P. e16761 - e16761

Published: Jan. 9, 2024

Background As one of the main pathogens causing tea anthracnose disease, Colletotrichum gloeosporioides has brought immeasurable impact on sustainable development agriculture. Given adverse effects chemical pesticides to environment and human health, biological control been a focus research this pathogen. Bacillus altitudinis GS-16, which was isolated from healthy leaves, had exhibited strong antagonistic activity against disease. Methods The antifungal mechanism endophytic bacterium GS-16 C. 1-F determined by dual-culture assays, pot experiments, cell membrane permeability, cellular contents, metabolism, activities key defense enzymes. Results We investigated possible strain inhibiting 1-F. In vitro , assays revealed that significant (92.03%) broad-spectrum in all tested plant pathogens. disease index decreased 6.12 after treatment with indicating good biocontrol effect (89.06%). When PE extract treated mycelial 1-F, appeared deformities, distortions, swelling SEM observations. Besides that, compared negative control, contents nucleic acids, protein, total soluble sugar group were increased significantly, could cause damage integrity also found obviously destroyed metabolism normal synthesis contents. Additionally, induced resistance increasing enzymes PPO, SOD, CAT, PAL, POD. Conclusions concluded permeability integrity, destroy induce resistance, contributed its activity. These findings indicated be used as an efficient microorganism for caused .

Language: Английский

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Biocontrol potential of the endophytic Epicoccum nigrum HE20 against stripe rust of wheat

Pesticide Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 194, P. 105517 - 105517

Published: July 6, 2023

Language: Английский

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Molecular identification and pathogenicity of endophytic fungi from corn ears

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 26, 2024

Endophytic fungi are widely known as that infect internal tissues of host plants for all or part their life cycles, without causing visible symptoms disease. The present study was carried out to identify and investigate the pathogenicity endophytic residing in husks, silks, kernels corn. were isolated from surface-sterilised kernels, husks healthy corn identified using sequencing multiple markers comprising TEF-1α, β-tubulin, calmodulin, ITS, LSU, ACT. A total 56 isolates belonging 17 species, namely Fusarium pseudocircinatum (n = 8), F. verticillioides 2), andiyazi 4), sacchari 1), mangiferae fujikuroi proliferatum 3), incarnatum Penicillium oxalicum P. polonicum citrinum 11), Aspergillus flavus 10), A. tubingensis Cladosporium tenuissimum Aureobasidium pullulans Curvularia lunata Epicoccum sorghinum 1) identified. Pathogenicity test showed induced varying severities disease on such leaf chlorosis necrosis, stem malformation, wilt, stunted growth with being most virulent. revealed harbour diverse genera can may cause harmful effects plants.

Language: Английский

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The invasion of Cassytha filiformis accelerated the litter decomposition of native plant communities in small tropical coral islands

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 21, 2025

Plant invasion affects plant community composition, biodiversity, and nutrient cycling in terrestrial ecosystems, particularly vulnerable ecosystems. As an invasive parasitic plant, Cassytha filiformis has caused extensive damage to the native vegetation of Paracel Islands. However, effects C. on litter decomposition release communities remain unclear. We conducted in-situ experiment a coral island explore dynamics varying across enzyme activities, soil properties degrees. The mass loss was determined during process. data showed that under severe significantly lower than uninvaded sites after nine months decomposition. accelerated nitrogen lignin with increased quality polyphenol oxidase activity. Besides, phosphorus availability potassium content also induced Meanwhile, organic carbon delayed because β-1, 4-glucosidase activity low first six months. peroxidase maintained high level plots, indicating residues may have allelopathy. Our results suggested element islands by regulating short-term rapid result loss, which is not conducive growth plants.

Language: Английский

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Combined interaction between the diazotrophic Niallia circulans strain YRNF1 and arbuscular mycorrhizal fungi in promoting growth of eggplant and mitigating root rot stress caused by Rhizoctonia solani

Phytopathologia Mediterranea, Journal Year: 2024, Volume and Issue: 63(1), P. 25 - 43

Published: April 9, 2024

Rhizoctonia root rot of eggplant, caused by solani, is an economically important disease. Niallia circulans YRNF1 and arbuscular mycorrhizal fungi (AMF) were assessed for their biocontrol biofertilizing effects against R. as potential replacements synthetic fungicides fertilizers. The diazotrophic N. YRNF1, isolated from soil, reduced in vitro growth solani 42%. GC-MS analysis culture filtrate detected bioactive compounds, including butyric acid (85%) ethylene glycol (8%). In greenhouse experiments, combined application AMF the severity eggplant 26%. This treatment triggered transcriptional expression five resistance genes (JERF3, PAL1, C3H, CHI2, HQT) treated eggplants. Biochemical analyses infected roots with bio-inoculants showed enhancement phenol content (+188%), increased antioxidant enzyme activity, mainly POD (+104%) PPO (+72%). Combined also promoted improved total NPK concentrations plant leaves. Inoculation presence AMR mycorrhization level. first report agents biological control promotion eggplant.

Language: Английский

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Eliciting transcriptomic and antioxidant defensive responses against Rhizoctonia root rot of sorghum using the endophyte Aspergillus oryzae YRA3

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Nov. 14, 2023

Environmental pollution due to the improper use of chemical fungicides represents a vital ecological problem, which affects human and animal health, as well microbial biodiversity abundance in soil. In this study, an endophytic fungus Aspergillus oryzae YRA3, isolated from wild plant Atractylis carduus (Forssk.) C.Chr, was tested for its biocontrol activity against Rhizoctonia root rot sorghum. The antagonistic potential A. YRA3 solani vitro. A full inhibition growth R. recorded indicating strong endophyte. To investigate composition metabolites, GC/MS analysis used thirty-two compounds culture filtrate were identified. Among these some with antifungal background detected including palmitic acid, 2-heptanone, 2,3-butanediol. metabolites can be attributed. greenhouse experiment, treating infected sorghum plants significantly reduced severity by 73.4%. An upregulation defensive genes (JERF3), (POD) (CHI II) roots when inoculated YRA3. addition, increment peroxidase polyphenol oxidase, total phenolic content also recorded. Furthermore, results obtained experiment revealed growth-promoting effect inoculating It concluded that probable biological agent control disease However, evaluation under field conditions is highly needed future studies.

Language: Английский

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Fusarium Induced Anatomical and Biochemical Alterations in Wild Type and DPA-treated Wheat Seedlings

Journal of Pure and Applied Microbiology, Journal Year: 2024, Volume and Issue: 18(1), P. 229 - 242

Published: Jan. 25, 2024

Wheat (Triticum aestivum) employs various strategies to defend against Fusarium oxysporum, a soil-borne vascular fungal pathogen that disrupts structural integrity and metabolism. The purpose of this research was ascertain the alterations anatomical biochemical responses in wild-type (WT) DPA-treated wheat (T. seedlings exposed F. oxysporum. WT showed disorganization parenchyma cells, sclerenchyma bundles (VBs), lower numbers xylem (Xy) phloem (Ph) reduced thickness cuticle layer (C) at epidermal shoots. content chlorophyll (Chl), carbohydrate, nucleic acid during infection. Enhanced defense through peroxidase (POD), polyphenol oxidase (PPO) observed be high as compared under stress condition. In addition, salicylic (SA) phenolics increased than DPA However, enhanced growth mycelia Hence, aspects decreased when

Language: Английский

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Plant growth-promoting microorganisms from native plants: an untapped resource of biocontrol and biofertilizer agents

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 29 - 66

Published: Jan. 1, 2024

Language: Английский

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Metabolomics analysis of mycelial exudates provides insights into fungal antagonists of Armillaria

Mycology&#58 An International Journal on Fungal Biology, Journal Year: 2023, Volume and Issue: 14(3), P. 264 - 274

Published: July 3, 2023

The genus Armillaria has high edible and medical values, with zones of antagonism often occurring when different species are paired in culture on agar media, while the antagonism-induced metabolic alteration remains unclear. Here, metabolome mycelial exudates two Chinese biological species, C G, co-cultured or cultured separately was analysed to discover candidate biomarkers key pathways involved antagonists. A total 2,377 metabolites were identified, mainly organic acids derivatives, lipids lipid-like molecules, organoheterocyclic compounds. There 248 142 differentially expressed between group C-G C, C-G, respectively, fourteen common including malate, uracil, Leu-Gln-Arg, etc. Metabolic like TCA cycle pyrimidine metabolism significantly affected by co-culture. Additionally, 156 new (largely derivatives) 32 potential antifungal compounds, primarily enriched into biosynthesis secondary identified co-culture mode. We concluded that malate uracil could be used as biomarkers, changes revealed this study provide insights mechanisms underlying fungal

Language: Английский

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Bio-stimulating effect of endophytic Aspergillus flavus AUMC 16068 and its respective ex-polysaccharides in lead stress tolerance of Triticum aestivum plant

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 25, 2024

Abstract Heavy metal accumulation is one of the major agronomic challenges that has seriously threatened food safety. As a result, metal-induced phytotoxicity concerns require quick and urgent action to retain maintain physiological activities microorganisms, nitrogen pool soils, continuous yields wheat in constantly worsening environment. The current study was conducted evaluate plant growth-promoting endophytic Aspergillus flavus AUMC 16,068 its EPS for improvement growth, phytoremediation capacity, consequences on plants ( Triticum aestivum ) under lead stress. After 60 days planting, heading stage plants, data growth metrics, properties, minerals content, content root, shoot, grains were recorded. Results evoked pollution reduced plants’ traits as well at all stress concentrations; however, inoculation with tolerant A. respective alleviated detrimental impact promoted characteristics lead-contaminated conditions also lowering oxidative through decreasing (CAT, POD, MDA , contrast growing un-inoculated polluted dealings. In conclusion, spores are regarded eco-friendly, safe, powerful inducers versus contamination heavy metals, view protecting plant, soil, human health.

Language: Английский

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