Characterization and Genetic analysis of Actinomycetes from Mangrove and Coastal Environments: Enzyme Production, Dye Degradation and Antibiotic Resistance DOI Open Access

P. Vanathi,

Uma Sundaresan, Kavitha Manivannan

et al.

Biosciences Biotechnology Research Asia, Journal Year: 2024, Volume and Issue: 21(4), P. 1553 - 1573

Published: Dec. 20, 2024

ABSTRACT: Actinomycetes from mangrove and coastal environments were studied for their potential in biotechnology environmental management. This research aimed to isolate characterize these microbes, focusing on enzyme production, dye degradation, antibiotic resistance. Samples collected various sites. isolated using selective media identified through morphological biochemical tests. Genetic characterization was performed 16S rRNA sequencing. Enzyme production evaluated specific proteases, lipases, cellulase assays. Dye degradation studies involved incubating actinomycetes with synthetic dyes measuring efficiency spectrophotometric methods. Antibiotic resistance assessed disk diffusion minimum inhibitory concentration (MIC) test. The study focused isolating a variety of environments, assessing degradation.

Language: Английский

A review on biodegradation of textile dye wastewater: Challenges due to wastewater characteristics and the potential of alkaliphiles DOI Creative Commons
Tadele Assefa Aragaw

Journal of Hazardous Materials Advances, Journal Year: 2024, Volume and Issue: 16, P. 100493 - 100493

Published: Oct. 9, 2024

Language: Английский

Citations

6

Biodegradation of azo dyes by Aspergillus flavus and its bioremediation potential using seed germination efficiency DOI Creative Commons

Amira Mohamed Ghanaim,

Omima M. El Mahdy,

Heba I. Mohamed

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 9, 2025

Abstract The worldwide textile industry extensively uses azo dyes, which pose serious health and environmental risks. Effective cleanup is necessary but challenging. Developing bioremediation methods for effluents will improve color removal efficiency. recent attention to effectively utilizing microbes convert toxic industrial dyes into non-hazardous compounds has garnered significant attention. In the present study, four fungal strains— Aspergillus flavus, terreus, niger , Fusarium oxysporium —were employed screen degradation detoxification of including congo red, crystal violet, bromophenol blue, malachite green. After eight days, A. flavus had degraded at maximum proportion. decolorization (%) was achieved 50 mg/L dye concentration, 8 days incubation, pH 6, 30 °C temperature, sucrose as a carbon source, NaNO 3 nitrogen Ca +2 minerals, using static culture. efficient production laccases, lignin peroxidase, manganese peroxidase enzymes by proved that enzyme played crucial role in decolorizing harmful dyes. Fourier Transform Infrared spectrometer (FT-IR) data validated process brought on absorption biodegradation. Compared control plants, results phytotoxicity assay showed product less maize common bean plant's growth germination rates. As result, findings indicate viable option remediating This aids biodegradation found wastewater.

Language: Английский

Citations

0

Production optimization, characterization, and application of a novel thermo- and pH-stable laccase from Bacillus drentensis 2E for bioremediation of industrial dyes DOI

Jothika Jeyabalan,

Ajithkumar Veluchamy, Selvaraju Narayanasamy

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142557 - 142557

Published: March 1, 2025

Language: Английский

Citations

0

Potential of Crude Extract of Streptomyces sp. nov., Strain TRM76147 for Control of A. gaisen DOI Open Access
Yihuang Chen, Jiaxin Zhang,

Guo Yang

et al.

Forests, Journal Year: 2024, Volume and Issue: 15(9), P. 1605 - 1605

Published: Sept. 11, 2024

Pear black spot, caused by A. gaisen during fruit growth, is a disease that significantly reduces pear yield. Biological control using antagonistic microorganisms regarded as viable alternative to chemical agents. The discovery of TRM76147, novel species Streptomyces isolated from the Taklamakan Desert, has demonstrated promising potential in addressing this issue. This study was conducted determine crude extract sp. nov., strain for gaisen. TRM76147 closely related griseoviridis NBRC 12874T, exhibiting an average nucleotide identity (ANI) value 82.13%. Combined with polyphasic taxonomic identification, suggests potentially new species. Through analyses BigSCAPE and antiSMASH, it determined genome contains 19 gene clusters. ethyl acetate demonstrates antifungal activity, active substance remaining stable at temperatures up 70 °C, achieving activity level 16.23 ± 0.22 mm. Furthermore, maintains its efficacy across pH range 2 12. Notably, diameter recorded 16.53 0.12 mm following 80 min UV irradiation. Under different treatment conditions, fermentation spore crumpling number reduction. In addition, also found broth could production spot disease, which initially revealed inhibition mechanism. abundant actinomycete resources have good application development bioactive substances biological control.

Language: Английский

Citations

0

Characterization and Genetic analysis of Actinomycetes from Mangrove and Coastal Environments: Enzyme Production, Dye Degradation and Antibiotic Resistance DOI Open Access

P. Vanathi,

Uma Sundaresan, Kavitha Manivannan

et al.

Biosciences Biotechnology Research Asia, Journal Year: 2024, Volume and Issue: 21(4), P. 1553 - 1573

Published: Dec. 20, 2024

ABSTRACT: Actinomycetes from mangrove and coastal environments were studied for their potential in biotechnology environmental management. This research aimed to isolate characterize these microbes, focusing on enzyme production, dye degradation, antibiotic resistance. Samples collected various sites. isolated using selective media identified through morphological biochemical tests. Genetic characterization was performed 16S rRNA sequencing. Enzyme production evaluated specific proteases, lipases, cellulase assays. Dye degradation studies involved incubating actinomycetes with synthetic dyes measuring efficiency spectrophotometric methods. Antibiotic resistance assessed disk diffusion minimum inhibitory concentration (MIC) test. The study focused isolating a variety of environments, assessing degradation.

Language: Английский

Citations

0