Impact of Vitamins, Antibiotics, Probiotics, and History of COVID-19 on the Gut Microbiome in Ulcerative Colitis Patients: A Cross-Sectional Study

Medicina, Journal Year: 2025, Volume and Issue: 61(2), P. 284 - 284

Published: Feb. 7, 2025

Background and Objectives: The human gut microbiome is essential for the health of host affected by antibiotics coronavirus disease 2019 (COVID-19). recognized as a contributing factor in development ulcerative colitis. Specific vitamins probiotics have been demonstrated to positively influence enhancing prevalence expected beneficial microorganisms. Materials Methods: Forty-nine colitis (UC) outpatients from Riga East Clinical University Hospital were enrolled this cross-sectional study June 2021 December 2021. All patients divided into groups based on history COVID-19 (COVID-19 positive vs. negative) last six months. Information about antibiotic, probiotic, vitamin intake outlined, faecal samples collected. MetaPhlAn v.2.6.0 tool was used taxonomic classification metagenome data. Statistical analysis performed using R 4.2.1. Results: Of 49 enrolled, 31 (63%) male 18 (37%) female. Coronavirus found 14 (28.6%) 6 Verrucomicrobia statistically significantly lower group (M = 0.05; SD 0.11) compared negative 0.5; 1.22), p 0.03. Antibiotic non-users had more Firmicutes their than antibiotic users (p 0.008). most supplement D (N 18), fifteen (42.9%) 3 (21.4%) over months > 0.05). Vitamin C (Md 72.8 [IQR: 66.6; 78.7] Md 60.1 42.4; 67.7]), 0.01. Conclusions: microbiome. Only significant results; users, observed. A mild course may not patients'

Language: Английский

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Culture-based characterization of gut microbiota in inflammatory bowel disease

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 20, 2025

Inflammatory bowel disease (IBD) is characterized by disruptions in the gut microbiome. While most studies on dysbiosis IBD rely sequencing-based methods, we employed a streamlined culturomics approach to obtain more comprehensive understanding of microbiota imbalance patients with that may not be captured sequencing alone. A total 367 bacteria were identified at species level, including 211 from ulcerative colitis patients, 164 Crohn's (CD) and 263 healthy individuals. Consistent our 16S rRNA gene amplicon results, significant decrease microbial diversity severe imbalance, especially CD also observed culture-based analysis. Our provided additional insights, highlighting unique anaerobic Gram-negative patients. Moreover, species-level findings for Bifidobacterium Enterobacterales emphasized specific expansions Notably, Mediterraneibacter gnavus, Thomasclavelia ramosa, Parabacteroides merdae, Collinsella aerofaciens are particular clinical interest due their correlation inflammatory biomarkers. This analysis underscores value integrating genome-based provide complementary insights therapeutic targets IBD.

Language: Английский

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Culturable Human Microorganisms and the Impact of Transportation Conditions on Cultivability

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 549 - 549

Published: Feb. 28, 2025

The composition of the human microbiome is a critical health indicator, and culture-independent methodologies have substantially advanced our understanding human-associated microorganisms. However, precise identification characterization microbial strains require culture-based techniques. Recently, resurgence culturomics, combined with high-throughput sequencing technology, has reduced high labor demand pure culture methods, facilitating more efficient comprehensive acquisition culturable strains. This study employed an integrated approach combining culturomic to identify microorganisms on scalp in saliva feces. Several Staphylococcus were identified from scalp, whereas anaerobic dominant fecal samples. Additionally, highlighted beneficial effects transportation conditions (liquid nitrogen treatment, dry ice transport, dimethyl sulfoxide [DMSO] buffer) preserving A robust methodology was developed for large-scale optimized transport that enhance potential isolating greater diversity

Language: Английский

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Compendium of Bifidobacterium-based probiotics: characteristics and therapeutic impact on human diseases

Microbiome Research Reports, Journal Year: 2024, Volume and Issue: 3(4)

Published: Oct. 8, 2024

The human microbiota, a complex community of microorganisms residing in and on the body, plays crucial role maintaining health preventing disease. Bifidobacterium species have shown remarkable therapeutic potential across range conditions, thus being considered optimal probiotic bacteria. This review provides insights into concept probiotics explores impact bifidobacteria health, focusing gastrointestinal, respiratory, skeletal, muscular, nervous systems. It also integrates information available genetic bases underlying beneficial effects each bifidobacterial different aspects physiology. Notably, -based proven effective managing gastrointestinal conditions such as constipation, antibiotic-associated diarrhea, irritable bowel syndrome (IBS), inflammatory disease (IBD), Helicobacter pylori infections. These benefits are achieved by modulating intestinal boosting immune responses, strengthening gut barrier. Moreover, been reported to reduce respiratory infections asthma severity. Additionally, these bacteria offer for skeletal muscular evidenced adolescentis breve , which anti-inflammatory symptom relief arthritis models, suggesting treating like rheumatoid arthritis. Furthermore, therapies based promising alleviating anxiety depression, reducing stress, enhancing cognitive function. Overall, this extensive scientific literature now that supports health-promoting applications underscores need further research confirm their clinical efficacy body

Language: Английский

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Gut Bacterial Composition and Nutritional Implications in Mexican and Spanish Individuals with Inflammatory Bowel Disease Compared to Healthy Controls

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 11887 - 11887

Published: Nov. 5, 2024

The intestinal microbiota plays a key role in the pathogenesis of inflammatory bowel disease (IBD), with its composition varying based on geographic location and dietary factors. This study was performed to examine compare bacterial gut Mexican Spanish individuals IBD healthy controls, while also considering nutritional aspects. involved 79 controls from Mexico Spain. fecal analyzed using 16S rRNA gene sequencing, intake anthropometric measurements were collected. Alpha diversity analysis revealed lower Chao1 index genera groups. Beta showed significant differences composition, suggesting inter-individual variability within Additionally, relative abundance varied across four

Language: Английский

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