Effects of surfactin stress on gene expression and pathological changes in Spodoptera litura DOI Creative Commons

Feiyan Zhang,

Qiuyue Liu, Yana Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 5, 2024

Spodoptera litura (S. litura) is a polyphagous pest of the family Lepidoptera, which causes damage and yields losses to many crops. The long-term use chemical pesticides for control not only seriously threatens environmental health, but also S. develop drug resistance. Therefore, there an urgent need environmentally safe friendly biogenic pesticides. However, mechanism action secondary metabolite (surfactin) Bacillus Vélezensis (B. vélezensis) on lepidopteran pests has been reported yet. We found that several metabolites genes in were affected by surfactin exposure. expressions (protoporphyrinogen (PPO), gluconolactone (GDL), L-cysteate) significantly down-regulated while glutamate hydroxychloroquine up-regulated. expression levels related metabolism detoxification, include glutathione s-transferase (GST) gene acetaldehyde dehydrogenase (ALDH), apoptosis-inhibiting (seven absentia homolog 1(SIAH1)) decreased. In addition, pathological changes occurred intestinal wall cells, Malpighian tubule nerve cells under stress. Conclusively, our results suggest induces increase reactive oxygen species (ROS) damages cells. Furthermore, based integrated analysis transcriptomic metabolomic data, it hypothesized may trigger neurotoxicity cardiotoxicity hindering insect's detoxification processes. This study lays foundation further exploration as potential biopesticide.

Language: Английский

Bio-Based Surfactants and Biosurfactants: An Overview and Main Characteristics DOI Creative Commons
Gloria Romero Vega, Paola Gallo Stampino

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 863 - 863

Published: Feb. 13, 2025

Natural surfactants are surface-active molecules synthesized from renewable resources (i.e., plants, animals, or microorganisms) and possess properties comparable to conventional surfactants, making them an environmentally friendly potential alternative petrochemical surfactants. Additionally, they exhibit biological such as anti-microbial properties, biodegradability, less toxicity, allowing their use in everyday products with minimal risk human health the environment. Based on mode of production, natural can be classified into first-generation bio-based second-generation biosurfactants, although definition may vary depending author literature. This review offers extensive classification focusing composition, sources, production methods, applications across various industries. Furthermore, main challenges future perspectives discussed.

Language: Английский

Citations

1
Bacillus velezensis CNPMS-22 as biocontrol agent of pathogenic fungi and plant growth promoter DOI Creative Commons
J. E. F. Figueiredo, Gisele de Fátima Dias Diniz, Mikaely Sousa Marins

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 5, 2025

Bacillus velezensis is a ubiquitous bacterium with potent antifungal activity and plant growth promoter. This study investigated the potential of B. CNPMS-22 as biocontrol agent against phytopathogenic fungi under diverse experimental conditions its Genome sequencing analysis revealed putative genes involved in these traits. research performed vitro experiments to evaluate antagonistic 10 using dual culture plate (DCP) inverted sealed assay (ISP). Greenhouse field tests evaluated ability control Fusarium verticillioides maize plants vivo. The genome was sequenced Illumina HiSeq 4,000 platform, genomic also included manual procedures identify interest accurately. showed all tested, notable reductions mycelial both DCP ISP experiments. In ISP, volatile organic compounds (VOCs) produced by altered mycelium coloration some fungi. Scanning electron microscopy morphological alterations hyphae F. contact CNPMS-22, including twisted, wrinkled, ruptured hyphae. Eight cluster candidates for synthesizing non-ribosomal lipopeptides ribosomal extracellular lytic enzymes, biofilm, VOCs, other secondary metabolites promoters were identified analysis. greenhouse that seed treatment reduced symptoms increased productivity. Our findings highlight CNPMS-22's bioinoculant fungal disease valuable implications sustainable crop

Language: Английский

Citations

0
Surfactin: a novel Aphis gossypii killing surfactin produced by Bacillus australimaris TRM82479 of Taklamakan Desert origin DOI Creative Commons
Y.X. Wang, Zhibin Sun, Shiyu Wang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 12, 2025

Introduction The cotton aphid Aphis gossypii poses a global, serious threat to yield and quality. Although chemical pesticides are effective, pollution resistance increasingly prominent, making development of new biopesticides priority in the context green agricultural development. Methods Given that reports on activity surfactins against A. limited, here, 107 Bacillus strains isolated from extreme environment Chinese Taklamakan Desert were screened for insecticidal using leaf-dip method. Active characterized by morphological observation, 16S rRNA gene sequencing, phylogenetic analysis. Secondary metabolite synthesis genes identified whole-genome sequencing antiSMASH Results B. australimaris strain TRM82479 showed 75.00% 48-h mortality . An analysis this contains several clusters related nonribosomal peptide (NRP) fengycin lichenysin lipopeptide analogs. Cluster 1 has highest similarity 52% with cluster, 8 92% cluster. It is inferred can produce analogs distinct lichenysin, so we its NRPs. results surfactin main substance, an LC 50 0.857 mg/mL 95 4.350 aphids aqueous solution. zebrafish acute toxicity experiment low-toxic fish, indicating good biological safety. Discussion This study not only provides resources control but also demonstrates potential as biopesticides, laying foundation their future application.

Language: Английский

Citations

0
Surfactin and poly–γ–glutamic acid co–production by Bacillus velezensis P#02 using a corn steep liquor–based medium DOI Creative Commons
Jéssica Correia, Eduardo J. Gudiña, Tomasz Janek

et al.

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 211, P. 109461 - 109461

Published: Aug. 9, 2024

Bacillus velezensis P#02 simultaneously produced surfactin and poly–γ–glutamic acid (γ–PGA). Among the different culture media studied, one containing corn steep liquor (100 mL/L), glucose (10 g/L), glutamic g/L) as sole ingredients (CSL–G–Glut(10)) offered best results regarding biosurfactant biopolymer production. Although production occurred both under shaking static conditions, significant only in cultures. Using medium CSL–G–Glut(10), 910 ± 20 mg surfactin/L 9.8 0.2 g γ–PGA/L were produced. Surfactin was synthetized a mixture of five homologues (fatty chains ranging between C12 C16), being most abundant C14– C15–surfactin. reduced surface tension up to 29 mN/m, with critical micelle concentration 52 mg/L, exhibited emulsifying activity. B. γ–PGA, which molecular weight around 229 kDa, displayed non–Newtonian shear–thinning profile, achieving apparent viscosity values 3800 mPa s aqueous solution, predominant viscous behavior. Accordingly, is promising strain for simultaneous γ–PGA using waste stream liquor.

Language: Английский

Citations

2
Isolation of Macrolactin A from a new Bacillus amyloliquefaciens and its aphicidal activity against Rhopalosiphum padi DOI

Kangbo Zhao,

Ruyi Ma,

Min Cheng

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract BACKGROUND Rhopalosiphum padi is a major infection affecting cereal crops in the boundary area. However, continuous use of chemical pesticides has increased cases drug resistance its field population. Therefore, we aimed to verify insecticidal properties Bacillus amyloliquefaciens YJNbs21.10 against aphids, isolated determine bioactivity metabolite Macrolactin A aphids for first time. RESULTS The results activity tracking showed that fermentation broth had best inhibitory efficacy R. , and corrected efficiency reached 95.57% after 24 h. With separation test, active components decreased: A, as most substance, control under 500 mg L −1 74.64% at 72 h, (which was significantly lower than broth, indicating synergistic effect between substances each part strain. In addition, result stereomicroscope damaged body wall aphids. toxicity confirmed through gradient test. CONCLUSION this study, exhibited comparable ability suggesting potential provide effective biological on also verified time, experiment, EC 50 substance 169.02 (24 h), which provided strong evidence may act an agent prevention aphid. © 2024 Society Chemical Industry.

Language: Английский

Citations

1
Effects of surfactin stress on gene expression and pathological changes in Spodoptera litura DOI Creative Commons

Feiyan Zhang,

Qiuyue Liu, Yana Wang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 5, 2024

Spodoptera litura (S. litura) is a polyphagous pest of the family Lepidoptera, which causes damage and yields losses to many crops. The long-term use chemical pesticides for control not only seriously threatens environmental health, but also S. develop drug resistance. Therefore, there an urgent need environmentally safe friendly biogenic pesticides. However, mechanism action secondary metabolite (surfactin) Bacillus Vélezensis (B. vélezensis) on lepidopteran pests has been reported yet. We found that several metabolites genes in were affected by surfactin exposure. expressions (protoporphyrinogen (PPO), gluconolactone (GDL), L-cysteate) significantly down-regulated while glutamate hydroxychloroquine up-regulated. expression levels related metabolism detoxification, include glutathione s-transferase (GST) gene acetaldehyde dehydrogenase (ALDH), apoptosis-inhibiting (seven absentia homolog 1(SIAH1)) decreased. In addition, pathological changes occurred intestinal wall cells, Malpighian tubule nerve cells under stress. Conclusively, our results suggest induces increase reactive oxygen species (ROS) damages cells. Furthermore, based integrated analysis transcriptomic metabolomic data, it hypothesized may trigger neurotoxicity cardiotoxicity hindering insect's detoxification processes. This study lays foundation further exploration as potential biopesticide.

Language: Английский

Citations

0