Demonstration of targeted crossovers in hybrid maize using CRISPR technology DOI Creative Commons
Andrei Kouranov, Charles Armstrong,

Ashok K. Shrawat

et al.

Communications Biology, Journal Year: 2022, Volume and Issue: 5(1)

Published: Jan. 13, 2022

Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage the plant breeding process by facilitating trait introgression, and potentially increasing rate gain. We present first demonstration targeted maize utilizing CRISPR Cas12a system. Our experiments showed that stable heritable can be produced F1 somatic cells using significantly higher than natural same interval. Molecular characterization recombinant demonstrated were driven non-homologous end joining (NHEJ) or HDR repair pathways, presumably mitotic cell cycle. These results step towards use RNA-guided nuclease technology simplify creation genome combinations progeny accelerate breeding.

Language: Английский

SorghumBase: a web-based portal for sorghum genetic information and community advancement DOI Creative Commons
Nicholas Gladman, Andrew Olson,

Sharon Wei

et al.

Planta, Journal Year: 2022, Volume and Issue: 255(2)

Published: Jan. 11, 2022

SorghumBase provides a community portal that integrates genetic, genomic, and breeding resources for sorghum germplasm improvement. Public research development in agriculture rely on proper data resource sharing within stakeholder communities. For plant breeders, agronomists, molecular biologists, geneticists, bioinformaticians, centralizing desirable into user-friendly hub crop systems is essential successful collaborations breakthroughs development. Here, we present the web ( https://www.sorghumbase.org ), community. hosts wide range of genomic information modular framework, built with open-source software, to provide sustainable platform. This initial release includes: (1) five reference genome assemblies pan-genome browser; (2) genetic variant natural diversity panels ethyl methanesulfonate (EMS)-induced mutant populations; (3) search interface integrated views various types; (4) links supporting interconnectivity other repositories including genebank, QTL, gene expression databases; (5) content management system support access news training materials. offers investigators improved collation will facilitate growth robust genomics-assisted breeding.

Language: Английский

Citations

28
Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass DOI Creative Commons
Guangchao Sun, Nishikant Wase, Shengqiang Shu

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Dec. 13, 2022

Abstract A number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how molecular mechanisms employed by these species be translated crops. Paspalum ( vaginatum ) self-incompatible and multiply stress-tolerant relative maize sorghum. Here, we describe sequencing pseudomolecule level assembly vegetatively propagated accession P. . Phylogenetic analysis based on 6,151 single-copy syntenic orthologues conserved 6 related grass places paspalum as an outgroup maize-sorghum clade. In parallel metabolic experiments, paspalum, but neither nor sorghum, exhibits significant increase trehalose when grown under nutrient-deficit conditions. Inducing accumulation maize, imitating phenotype results autophagy dependent increases biomass accumulation.

Language: Английский

Citations

27
The Grape Gene Reference Catalogue as a Standard Resource for Gene Selection and Genetic Improvement DOI Creative Commons
David Navarro‐Payá, Antonio Santiago, Luis Orduña

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 12

Published: Jan. 17, 2022

Effective crop improvement, whether through selective breeding or biotech strategies, is largely dependent on the cumulative knowledge of a species' pangenome and its containing genes. Acquiring this specially challenging in grapevine, one oldest fruit crops grown worldwide, which known to have more than 30,000 Well-established research communities studying model organisms created maintained, public private funds, diverse range online tools databases serving as repositories genomes gene function data. The lack such resources for non-model, but economically important, Vitis vinifera species has driven need standardised collection genes within grapevine community. In an effort led by Integrape COST Action CA17111, we recently developed first grape reference catalogue, where are ascribed functional data, including their accession identifiers from different genome-annotation versions (https://integrape.eu/resources/genes-genomes/). We present discuss repository together with validation-level scheme based varied supporting evidence found current literature. catalogue structure submission form provided permits community curation. Finally, Gene Cards tool, Visualization (VitViz) platform, visualize data collected link tissue-specific expression derived transcriptomic This perspective article aims these well highlight potential use, particular plant-breeding applications.

Language: Английский

Citations

26
Genomic Analysis of Resistance to Fall Armyworm (Spodoptera frugiperda) in CIMMYT Maize Lines DOI Open Access

Isaac Kamweru,

Bruce Y. Anani,

Yoseph Beyene

et al.

Genes, Journal Year: 2022, Volume and Issue: 13(2), P. 251 - 251

Published: Jan. 28, 2022

The recent invasion, rapid spread, and widescale destruction of the maize crop by fall armyworm (FAW; Spodoptera frugiperda (J.E. Smith)) is likely to worsen food insecurity situation in Africa. In present study, a set 424 lines were screened for their responses FAW under artificial infestation dissect genetic basis resistance. All evaluated two seasons screen houses genotyped with DArTseq platform. Foliar damage was rated on scale 1 (highly resistant) 9 susceptible) scored at 7, 14, 21 days after infestation. Analyses variance revealed significant genotypic genotype environment interaction variances all traits. Heritability estimates leaf scores moderately high ranged from 0.38 0.58. Grain yield negatively correlated magnitude foliar scores, ear rot, genome-wide association study (GWAS) 56 marker–trait associations predicted functions putative candidate genes varied defense response several unknown function. Overall, that native resistance quantitative nature controlled many loci minor effects.

Language: Английский

Citations

24
Demonstration of targeted crossovers in hybrid maize using CRISPR technology DOI Creative Commons
Andrei Kouranov, Charles Armstrong,

Ashok K. Shrawat

et al.

Communications Biology, Journal Year: 2022, Volume and Issue: 5(1)

Published: Jan. 13, 2022

Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage the plant breeding process by facilitating trait introgression, and potentially increasing rate gain. We present first demonstration targeted maize utilizing CRISPR Cas12a system. Our experiments showed that stable heritable can be produced F1 somatic cells using significantly higher than natural same interval. Molecular characterization recombinant demonstrated were driven non-homologous end joining (NHEJ) or HDR repair pathways, presumably mitotic cell cycle. These results step towards use RNA-guided nuclease technology simplify creation genome combinations progeny accelerate breeding.

Language: Английский

Citations

23