Genome-wide identification, stress- and hormone-responsive expression characteristics, and regulatory pattern analysis of Scutellaria baicalensis SbSPLs

Plant Molecular Biology, Journal Year: 2024, Volume and Issue: 114(2)

Published: Feb. 16, 2024

Abstract SQUAMOSA PROMOTER BINDING PROTEIN-LIKEs ( SPLs ) encode plant-specific transcription factors that regulate plant growth and development, stress response, metabolite accumulation. However, there is limited information on Scutellaria baicalensis . In this study, 14 SbSPLs were identified divided into 8 groups based phylogenetic relationships. in the same group had similar structures. Abscisic acid-responsive (ABRE) MYB binding site (MBS) cis-acting elements found promoters of 6 Segmental duplications transposable main causes SbSPL expansion. Expression analysis transcriptional profiling showed SbSPL1 , SbSPL10 SbSPL13 highly expressed roots, stems, flowers, respectively. quantitative real-time polymerase chain reaction (RT‒qPCR) most responded to low temperature, drought, abscisic acid (ABA) salicylic (SA), among which expression levels SbSPL7/9/10/12 significantly upregulated response abiotic stress. These results indicate are involved growth, development S. addition, Sba-miR156/157 s identified, SbSPL1-5 was a potential target s. The gene prediction coexpression together indicated may be regulation L-phenylalanine (L-Phe), lignin jasmonic (JA) biosynthesis. summary, identification characterization family lays foundation for functional research provides reference improved breeding resistance quality traits.

Language: Английский

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Identification and Characterization of SQUAMOSA Promoter Binding Protein-like Transcription Factor Family Members in Zanthoxylum bungeanum and Their Expression Profiles in Response to Abiotic Stresses

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 520 - 520

Published: Feb. 8, 2025

Plant-specific transcription factors known as SQUAMOSA promoter binding protein-like (SPL) genes are essential for development, growth, and abiotic stress responses. While the SPL gene family has been extensively studied in various plant species, a systematic characterization Zanthoxylum bungeanum (Zb) is lacking. This study used transcriptomic bioinformatics data to conduct thorough genomic identification expression investigation of ZbSPL family. Eight subfamilies including 73 members were identified, most which predicted be localized nucleus. Ka/Ks ratio analysis indicates that have undergone purifying selection. According evolutionary research, segmental duplication major factor amplification Gene structures, conserved motifs, domains found highly among paralogs. Cis-element research revealed ZbSPLs may implicated hormone Codon usage pattern showed was more inclined A/T base endings; higher content, stronger preference codons; use mainly affected by natural Additionally, 36 potential targets miR156. RNA-seq demonstrated Zb differentially expressed response distinct stressors. Two (ZbSPL10 ZbSPL17) salt stress, while four (ZbSPL06, ZbSPL43, ZbSPL60, ZbSPL61) drought based on qRT-PCR under conditions. will help us gain deeper understanding functions lay genetic foundation future breeding high-quality, resistant varieties Z. bungeanum.

Language: Английский

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Integrated RNA-Seq and Metabolomics Analyses of Biological Processes and Metabolic Pathways Involved in Seed Development in Arachis hypogaea L.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 300 - 300

Published: Feb. 28, 2025

In peanut cultivation, fertility and seed development are essential for fruit quality yield, while pod number per plant, pod, kernel weight, size indicators of yield. this study, metabolomic RNA-seq analyses were conducted on the flowers aerial pegs (aerpegs) two cultivars JNH3 (Jinonghei) SLH (Silihong), respectively. Compared with SLH, had 3840 up-regulated flower-specific differentially expressed genes (DEGs) 5890 aerpeg-specific DEGs. aerpegs, there 4079 variety-specific DEGs 18 accumulated metabolites (DAMs) flowers, 3732 48 DAMs in flowers. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment revealed that associated pollen germination phenylalanine metabolism flower aerpeg tissues, contrast, protein degradation, amino acid metabolism, DNA repair. However, significant differences lipids lipid-like molecules between This investigation provides candidate an experimental basis further improvement high-quality high-yield varieties.

Language: Английский

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CsSPL13A directly binds and positively regulates CsFT and CsBAM to accelerate flowering in cucumber

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 207, P. 108395 - 108395

Published: Jan. 28, 2024

Language: Английский

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Genome-wide identification and expression analysis of the SPL transcription factor family and its response to abiotic stress in Pisum sativum L

BMC Genomics, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 31, 2024

Abstract Squamous promoter binding protein-like ( SPL ) genes encode plant-specific transcription factors (TFs) that play essential roles in modulating plant growth, development, and stress response. Pea Pisum sativum L. is a coarse grain crop of great importance food production, biodiversity conservation molecular genetic research, providing information nutritional resources for improving agricultural production promoting human health. However, only limited researches on the structure functions exist pea PsSPLs ). In this study, we identified 22 conducted genome-wide analysis their physical characteristics, chromosome distribution, gene structure, phylogenetic evolution expression patterns. As result, were unevenly distributed seven chromosomes harbored SBP domain, which composed approximately 76 amino acid residues. The revealed clustered into eight subfamilies showed high homology with soybean. Further presence segmental duplications . patterns at different tissues, developmental stages under various stimulus conditions evaluated by qRT-PCR method. It was found from same subfamily similar transcripts most reached maximum peak value 14 days after anthesis pod. Abiotic stresses can cause significantly up-regulated PsSPL19 spatiotemporal specificity, addition, four hormones including time-dependent manner. Therefore, could be key candidate signal transduction during growth pod formation, abiotic hormone Our findings should provide insights elucidating development regulation mechanism breeding resistance to pea.

Language: Английский

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Identification of the BBX gene family in blueberry at different chromosome ploidy levels and fruit development and response under stress

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 3, 2025

Language: Английский

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Fine mapping and candidate gene analysis of major QTLs for number of seeds per pod in Arachis hypogaea L.

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 15, 2025

Peanut (Arachis hypogaea L., 2n = 2x 20) is an important industrial and oil crop that widely grown in more than 100 countries. In recent years, breeders have focused on increasing the seed number per pod to improve their yield addition other breeding for key components of yield, including number, seeds pod, 100-seed weight. this study, a secondary population 1,114 BC1F2 lines was derived from cross between parents R45 JNH3. Two stable major-effect quantitative trait loci qRMPA09.1 qRMPA09.2 were detected simultaneously mapped within chromosomal intervals approximately 400 Kb 600 chromosome A09. Additionally, combined whole-genome RNA sequencing analyses showed differential expression Arahy.04JNDX gene belongs MYB transcription factor (TF) two parents. The AhMYB51 also inferred influence peanuts. An examination backcross L2/L4 increases rate multiple pods plant (RMSP) primarily by affecting brassinosteroids flowers, while its overexpression promotes length siliques Arabidopsis thaliana. Our findings provide valuable insights cloning favorable alleles RMSP qRMSPA09.1 qRMSPA09.2 are novel QTL associated with trait, predicted as candidate gene. Moreover, closely linked polymorphic SNP markers significant QTLs may be useful accelerating marker-assisted

Language: Английский

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Genome-Wide Identification and Expression Analysis of the SPL Gene Family in Three Orchids

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(12), P. 10039 - 10039

Published: June 12, 2023

SPL transcription factors regulate important processes such as plant growth and development, metabolic regulation, abiotic stress. They play crucial roles in the development of flower organs. However, little is known about characteristics functions SPLs Orchidaceae. In this study, Cymbidium goeringii Rchb. f., Dendrobium chrysotoxum Lindl., Gastrodia elata BI. were used research objects. The gene family these orchids was analyzed on a genome-wide scale, their physicochemical properties, phylogenetic relationships, structures, expression patterns studied. Transcriptome qRT-PCR methods combined to investigate regulatory effect organs during flowering process (bud, initial bloom, full bloom). This study identifies total 43 from C. (16), D. (17), G. (10) divides them into eight subfamilies according tree. Most proteins contained conserved SBP domains complex structures; half genes had introns longer than 10 kb. largest number variety cis-acting elements associated with light reactions enriched, accounting for 45% (444/985); 13/43 contain response miRNA156. GO enrichment analysis showed that most mainly enriched stems. addition, suggested involvement regulation organ orchids. There change CgoSPL goeringii, but DchSPL9 GelSPL2 significant elata, respectively. summary, paper provides reference exploring

Language: Английский

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Possible Mechanism of Sucrose and Trehalose-6-Phosphate in Regulating the Secondary Flower on the Strong Upright Spring Shoots of Blueberry Planted in Greenhouse

Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2350 - 2350

Published: Aug. 23, 2024

Secondary flowering is the phenomenon in which a tree blooms twice or more times year. Along with development of blueberry (

Language: Английский

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CpSPL10-CpELF4 module involves in the negative regulation of flower bud differentiation in Chinese cherry

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 135964 - 135964

Published: Sept. 1, 2024

Language: Английский

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