An integrative approach clarifies species delimitation and biogeographic history of Debregeasia (Urticaceae) DOI Creative Commons
Amos Kipkoech, Ke Li, Richard I. Milne

et al.

Plant Diversity, Journal Year: 2024, Volume and Issue: 47(2), P. 229 - 243

Published: Nov. 26, 2024

Integrative data from plastid and nuclear loci are increasingly utilized to resolve species boundaries phylogenetic relationships within major angiosperm clades. Debregeasia (Urticaceae), an economically important genus, presents challenges in delimitation due its overlapping morphological traits unstable taxonomic assignments. Here, we analyzed 14 generated 12 matrices the plastomes nrDNA using genome skimming nine recognized morphospecies clarify assess barcode performance Debregeasia. We also used a universal set of 353 genes explore reticulate evolution biogeographic history Plastomes exhibited typical quadripartite structure with conserved gene content marginal independent variations SC/IR boundary at inter- intra-specific levels. Three were non-monophyletic could not be discerned by any barcode; however, ultra-barcodes identified remaining six (67%), outperforming standard barcodes (56%). Our analyses placed wallichiana outside genus suggested monophyletic clades Debregeasia, although placement between hekouensis libera varied. There was extensive trait overlap key morphologically diagnostic characters, reticulation analysis showing potentially pervasive hybridization, likely influenced speciation patterns overlaps ranges. inferred that crown diversification began ca. 12.82 Ma (95% HPD: 11.54-14.63 Ma) mid-Miocene Australia, followed vicariance later long-distance dispersal, mainly out southern China. findings highlight utility genomic integrative lines evidence refine evolutionary complex plant lineages.

Language: Английский

Plastome Sequences Uncover the Korean Endemic Species Polygonatum grandicaule (Asparagaceae) as Part of the P. odoratum Complex DOI Open Access

Joonhyung Jung,

Hyuk-Jin Kim,

Joo‐Hwan Kim

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(4), P. 398 - 398

Published: March 29, 2025

Background/Objectives: Polygonatum grandicaule Y.S.Kim, B.U.Oh & C.G.Jang (Asparagaceae Juss.), a Korean endemic species, has been described based on its erect stem, tubular perianth shape, and pedicel length. However, taxonomic status remains unclear due to limited molecular data. Methods: This study presents the complete plastid genomes (plastomes) of two P. individuals close relative, odoratum (Mill.) Druce var. thunbergii (C.Morren Decne.) H.Hara. Results: The plastomes, ranging from 154,578 154,579 base pairs (bp), are identical those falcatum A.Gray, odoratum, another infundiflorum C.G.Jang. All contain 78 protein-coding genes (PCGs), 30 tRNA genes, four rRNA except for pseudogene infA. Phylogenetic analyses using PCGs whole intergenic spacer (IGS) regions strongly support three sections within Mill. show that variety nested falcatum, grandicaule, infundiflorum. Conclusions: Given genomic variation phylogenetic relationships, we propose treating as part complex, despite their morphological differences. offers valuable putative markers species identification supports application plastome-based super-barcoding in morphologically diverse genus Polygonatum.

Language: Английский

Citations

0
An integrative approach clarifies species delimitation and biogeographic history of Debregeasia (Urticaceae) DOI Creative Commons
Amos Kipkoech, Ke Li, Richard I. Milne

et al.

Plant Diversity, Journal Year: 2024, Volume and Issue: 47(2), P. 229 - 243

Published: Nov. 26, 2024

Integrative data from plastid and nuclear loci are increasingly utilized to resolve species boundaries phylogenetic relationships within major angiosperm clades. Debregeasia (Urticaceae), an economically important genus, presents challenges in delimitation due its overlapping morphological traits unstable taxonomic assignments. Here, we analyzed 14 generated 12 matrices the plastomes nrDNA using genome skimming nine recognized morphospecies clarify assess barcode performance Debregeasia. We also used a universal set of 353 genes explore reticulate evolution biogeographic history Plastomes exhibited typical quadripartite structure with conserved gene content marginal independent variations SC/IR boundary at inter- intra-specific levels. Three were non-monophyletic could not be discerned by any barcode; however, ultra-barcodes identified remaining six (67%), outperforming standard barcodes (56%). Our analyses placed wallichiana outside genus suggested monophyletic clades Debregeasia, although placement between hekouensis libera varied. There was extensive trait overlap key morphologically diagnostic characters, reticulation analysis showing potentially pervasive hybridization, likely influenced speciation patterns overlaps ranges. inferred that crown diversification began ca. 12.82 Ma (95% HPD: 11.54-14.63 Ma) mid-Miocene Australia, followed vicariance later long-distance dispersal, mainly out southern China. findings highlight utility genomic integrative lines evidence refine evolutionary complex plant lineages.

Language: Английский

Citations

2