Smart estimation of protective antioxidant enzymes’ activity in savory (Satureja rechingeri L.) under drought stress and soil amendments

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 6, 2025

Savory (Satureja rechingeri L.) is one of Iran's most important medicinal plants, having low irrigation needs, and thus considered the valuable plants for cultivation in arid semi-arid regions, especially under drought conditions. The current research was carried out to develop a genetic algorithm-based artificial neural network (ΑΝΝ) model able simulating levels antioxidants savory when using soil amendments [biochar (BC) superabsorbent (SA)] drought. Data different watering schemes showed that both BC SA have mitigating effects over stress by optimizing enzymatic non-enzymatic antioxidant traits (POD, CTA, APX enzymes). Specifically, biochar led improved homeostasis water deficit as reflected lower MDA levels. An ANN with 3-10-6 topology found be best predict polyphenols (PHE), proline (PRO), peroxidase (POX), catalase (CAT), ascorbate (APX) levels, indicator oxidative malondialdehyde (MDA). model's efficiency established R-value statistical parameter, simulated GA-ANN data were highly correlated experimental findings. Across antioxidants, had fit, an 0.9733. On other hand, POX predictive correlation (R = 0.8737), indicating capacity system forecasting this parameter. 0.9690) elevated assimilation performance PHE 0.9604) PRO 0.9245) study shows potential predicting content non-invasive, low-cost alternative.

Language: Английский

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Refinement of surface sterilization protocol for in vitro olive (Olea europaea L.) shoot proliferation and optimizing by machine learning techniques

Horticulture Environment and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 18, 2025

Language: Английский

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Transcriptional modification and the accumulation of flavonoid in the leaves of Cissus rotundifolia Lam. in respond to drought stress

Stress Biology, Journal Year: 2025, Volume and Issue: 5(1)

Published: March 10, 2025

Abstract Cissus rotundifolia Lam. is an evergreen climber that mainly distributed in the tropical savannas of eastern Africa with excellent drought resistance. While mechanism underlying stress response C. remains unknown. In this study, we investigated transcriptomics and flavonoid metabolomics responses leaves under different conditions. We identified a total 2401 differentially expressed genes (DEGs) drought-treated leaves. The continuously increasing DEGs were significantly enriched pathways related to phenylpropanoid biosynthesis, galactose metabolism. flavonoids content also found increased during treatment . A 57 distinct using UPLC-MS/MS. Among them, nine out ten accumulated metabolites (DAMs) displayed notable accumulation profiles These DAMs included two flavanols [(-)-Catechin gallate (-)-gallocatechin gallate], flavonols (myricetin astragalin), four flavones (orientin, cynaroside, isoorientin, vitexin), one flavanone (naringenin-7-glucoside), indicating their pivotal roles response. Additionally, DEG high-expression, annotated flavone synthase ( FLS ) synthesises, synthetizing myricetin was tightly additional belonging ABA-signaling genes, stomata movement transcription factors, protein kinases, these contained Top 10 hub-genes network constructed all DEGs. results reflect significance potentially regulatory findings suggest play role , advancing our understanding transcriptional regulation involved process.

Language: Английский

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Gamma amino butyric acid (GABA) application modulated the morpho-physiological and yield traits of fragrant rice under well-watered and drought conditions

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: June 18, 2024

Abstract Background Changing climate is causing erratic rainfall and prolonged drought periods, thus posing serious threats to crop productivity. Owing severity of events, it imperative take proactive measures enhance the resilience sensitive crops like rice. Therefore, present study was carried out improve stress tolerance in rice through gamma amino butyric acid (GABA) application. Methods The experiment included four GABA concentrations i.e., 0 mM as control, 1 mM, 1.5 2 two water levels 100% 50% field capacity (referred FC100 for well-watered FC50 conditions, respectively), fragrant cultivars Super Basmati Basmati-515. Results findings unveiled a comprehensive improvement various parameters with application under both (FC100) water-limited (FC50) compared control. Specifically, induced enhancements were observed plant height, root length, fresh weight, dry total soluble protein content, free content across cultivars. Moreover, significantly improved peroxidase (POD) catalase (CAT) enzyme activities, alongside elevating anthocyanin levels, while concurrently reducing H O contents treatments. Furthermore, positive impact extended morphological traits, notable increases panicle tillers productive per hill, branch grain numbers panicle, 1000-grain weight 515 regimes, Ck. Similarly, yield increased by 31.01% 27.32% 36.85% 27.71% Basmati-515, respectively, response application, Additionally, principal component analysis (PCA) revealed significant variances attributed Dim1 Dim2, 86.1% 4.0% variance, three bi-plots encompassing cultivars, Notably, all tested indices, except non-productive exhibited correlations amongst themselves yield, further emphasizing beneficial effects on conditions. Conclusions performance integrating into cultivation practices could not only but also potentially benefiting future food nutritional security globally. However, however; research needed understand cellular molecular mechanisms functionality rice, particularly

Language: Английский

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Leveraging machine learning to unravel the impact of cadmium stress on goji berry micropropagation

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(6), P. e0305111 - e0305111

Published: June 13, 2024

This study investigates the influence of cadmium (Cd) stress on micropropagation Goji Berry (Lycium barbarum L.) across three distinct genotypes (ERU, NQ1, NQ7), employing an array machine learning (ML) algorithms, including Multilayer Perceptron (MLP), Support Vector Machines (SVM), Random Forest (RF), Gaussian Process (GP), and Extreme Gradient Boosting (XGBoost). The primary motivation is to elucidate genotype-specific responses Cd stress, which poses significant challenges agricultural productivity food safety due its toxicity. By analyzing impacts varying concentrations plant growth parameters such as proliferation, shoot root lengths, numbers, we aim develop predictive models that can optimize under adverse conditions. ML revealed complex relationships between exposure physiological changes, with MLP RF showing remarkable prediction accuracy (R2 values up 0.98). Our findings contribute understanding heavy metal offer practical applications in mitigating plants, demonstrating potential approaches advancing tissue culture research sustainable practices.

Language: Английский

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GABA and Its Crosstalk with Other Metabolites in Relation to Abiotic Stress Responses in Plants

Published: Jan. 10, 2025

Gamma-aminobutyric acid (GABA), a universal 4-carbon amino that is absent from proteins, present in both prokaryotic and eukaryotic living forms. It commonly known this plays crucial role plant functioning acts as neurotransmitter human cells. The focus of chapter on GABA's capacity to lessen the negative effects abiotic stressors resulting climate change, including exposure heat, salt, chilling, drought, heavy metals. These stresses hampers growth yield crops via enhancing ROS induced damages lowering water content, photosynthesis, stomatal conductance, fresh biomass etc. In plants, GABA maintain several biochemical physiological processes like activation antioxidant defence, transporters proteins genes shield plants range challenging conditions. This comprehensive study investigates production pathway, intricate interactions with various stresses, transportation systems. Additionally covered complex relationship between phytohormones environmental stress adjustment, which offers important insights for strategic planning stress-reduction methods. analysis different signalling molecules calcium, hydrogen peroxide, nitric oxide reveals how it creating strategies will help withstand stressors.

Language: Английский

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The ERF transcription factor TaERF13-2B functions as a negative regulator of drought tolerance in Arabidopsis and wheat

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 27, 2025

Ethylene response factors (ERFs) are transcription that essential in modulating drought stress responses plants such as Arabidopsis and rice. However, the functional role of ERF wheat remains unclear. We identified 33 genes under using transcriptomic analysis categorized them into eight subfamilies (I–VIII). Among them, 12 drought-responsive candidate were upregulated, TaERF13-2B was selected for further analysis. overexpression resulted significantly reduced survival rates conditions with decreased expression stress-responsive antioxidant enzyme genes, indicating gene elevated sensitivity transgenic . In wheat, increased malondialdehyde accumulation, chlorophyll proline levels, activity. Furthermore, antioxidant-related suppressed, suggesting negatively regulates to stress. The interactions between TaCIPK9 confirmed yeast two-hybrid bimolecular fluorescence complementation. Overall, these discoveries deepen our insights family contribute elucidation wheat.

Language: Английский

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Combined Application of Plant Growth Promoting Rhizobacteria and Kinetin on Maize Growth, Chlorophyll, Osmoregulation, and Oxidative Metabolism Under Drought Stress

Journal of soil science and plant nutrition, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Language: Английский

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Comparative study of the ability of green synthesized Se-NPs and CTS-NPs to overcome drought stress in Oryza sativa L. for regenerative nanoengineering in agriculture

New Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Green synthesized NPs derived from Acacia modesta leaves, primed on seeds, confirmed the potential to mitigate drought stress under hydroponic system.

Language: Английский

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Inoculation with Micromonospora sp. enhances carbohydrate and amino acid production, strengthening antioxidant metabolism to mitigate heat stress in wheat cultivars

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 19, 2024

Introduction Heat stress caused by global warming adversely affects wheat yield through declining most nutritional quality attributes in grains, excluding grain protein content. Methods This research investigated the biochemical, physiological, and antioxidant responses of plants under heat stress, focusing on role plant growth-promoting bacteria ( Micromonospora sp.). Two genotypes were studied: one heat-sensitive heat-tolerant, examining their to with without bacterial inoculation. Results Under sensitive cultivar experienced significant reductions photosynthesis rate, chlorophyll content, RuBisCO activity (57-61%), while tolerant had milder (24-28%). sp. treatment notably improved these parameters (+48-78%), resulting a substantial increase biomass production (+43-53%), which was not seen cultivar. Additionally, oxidative markers (H 2 O MDA) elevated more (82% 90% higher) compared one. effectively reduced (-28% -27%). Enhanced enzymes ASC-GSH pathway particularly evident sp.-treated plants. Carbohydrate metabolism shifted, increased soluble sugars rises sucrose content stress. Discussion The higher sugar levels facilitated amino acid synthesis, contributing biosynthesis secondary metabolites, including flavonoids, polyphenols, anthocyanins. reflected phenylalanine ammonia-lyase, cinnamate (CA) 4-hydroxylase, chalcone synthase enzymes, indicating activation phenylpropanoid pathways. Overall, findings suggest that can mitigate effects enhancing photosynthetic efficiency, defense, metabolic adaptations cultivars.

Language: Английский

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