Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals that NtDnaJA3 responses to drought stress DOI Creative Commons
Qiao Wang,

Lijun Meng,

Zechao Qu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract Background DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on family have been conducted in plants. However, research this tobacco remains relatively scarce. Results In study, we identified 24 genes tobacco, and classified them into three individual groups. A comprehensive analysis based gene structure, motif composition, evolutionary pattern revealed divergence genes. For evolution NtDnaJA genes, purification selection was major factor. addition, potential regulatory network unveiled NtDnaJAs could be regulated by miRNAs transcription factors associated with diverse stress responses. Through expression qRT-PCR experiments, it observed many NtDnaJAs displayed tissue-specific might play significant roles different biotic abiotic Additionally, pivotal role NtDnaJA3 boosting plant drought resistance confirmed. Conclusions This study provides important perspectives their involvement responses, laying groundwork for future tobacco. Graphical Abstract

Language: Английский

Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals NtDnaJA3 responses to drought stress DOI Creative Commons
Qiao Wang,

Lijun Meng,

Zechao Qu

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 7, 2025

DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on family have been conducted in plants. However, research this tobacco remains relatively scarce. In study, we identified 24 genes tobacco, and classified them into three individual groups. A comprehensive analysis based gene structure, motif composition, evolutionary pattern revealed divergence genes. For evolution NtDnaJA genes, purification selection was major factor. addition, potential regulatory network unveiled NtDnaJAs could be regulated by miRNAs transcription factors associated with diverse stress responses. Through expression public RNA-seq datasets qRT-PCR experiments, it observed many displayed tissue-specific might play significant roles different biotic abiotic Additionally, pivotal role NtDnaJA3 boosting plant drought resistance confirmed. This study provides important perspectives their involvement responses, laying groundwork for future tobacco.

Language: Английский

Citations

0
Characterization of the m6A Regulatory Gene Family in Phaseolus vulgaris L. and Functional Analysis of PvMTA in Response to BCMV Infection DOI Open Access
Wenyan Wu, Xinhua Wang,

Xingrui Liang

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2748 - 2748

Published: March 19, 2025

Common bean (Phaseolus vulgaris L.) is known for its high protein, dietary fiber, and various trace element contents, making it a widely grown leguminous crop globally. The common mosaic virus (BCMV) poses significant threat to production, causing substantial yield reductions when beans are infected. Widely occurring in mRNA, the m6A modification vital maintaining mRNA stability, facilitating splicing, enabling nuclear export, supporting polyadenylation, initiating translation. Recent studies have identified regulatory gene family plant species, ability regulate infection has been confirmed. There currently insufficient information regarding how responds BCMV infection. Consequently, we carried out genome-wide characterization of bean, which led identification 31 potential members associated with m6A. According evolutionary analysis, increase appears be linked either whole-genome duplication or segmental events. Subsequent investigations into expression levels these genes throughout different phases showed that all candidate responded changes expression. Moreover, characterized methyltransferase activity PvMTA validated interactive relationship between adenosine A (MTA) B (MTB) beans. Through overexpressing silencing PvMTA, further ascertained this particular detrimental impact on regulation This research provides fresh perspectives molecular processes govern interaction aids progress breeding.

Language: Английский

Citations

0
Genome-wide identification and analysis of the TBL genes reveals NtTBL31 increases drought resistance of tobacco (Nicotiana tabacum) DOI Creative Commons

Sujie Wang,

Huan Su, Jingjing Jin

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 22, 2025

Language: Английский

Citations

0
A comprehensive review of m6A modification in plant development and potential quality improvement DOI

Chaofan Shan,

Kui Dong,

Dongyu Wen

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142597 - 142597

Published: March 1, 2025

Language: Английский

Citations

0
Genome-wide analysis of the SWEET gene family in Hemerocallis citrina and functional characterization of HcSWEET4a in response to salt stress DOI Creative Commons
Lihong Cao, Jinyao Wang, Lixuan Wang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 11, 2024

Abstract Sugars will be eventually effluxed transporters ( SWEETs ) have been confirmed to play diverse physiological roles in plant growth, development and stress response. However, the characteristics functions of SWEET genes Hemerocallis citrina remain unclear poorly elucidated. In this study, whole genome was utilized conduct bioinformatics analysis a total 19 HcSWEET were successfully identified. Analysis physicochemical properties indicated dominant differences among these HcSWEETs . A phylogenetic revealed that proteins can divided into 4 clades ranging from Clade I IV, where within same clade exhibited shared conserved motifs gene structures. Five six exons contained majority genes, which unevenly distributed across 11 chromosomes. The duplication showed presence pairs. Comparative syntenic maps family might present more closed homology monocotyledons than dicotyledons. Cis-acting element key responsiveness various hormones, light, stresses. Additionally, transcriptome sequencing suggested most had relatively higher expression roots, HcSWEET4a significantly up-regulated under salt stress. Overexpression further verified possibility involved response stress, provides novel insights facilitates in-depth studies functional resistance abiotic

Language: Английский

Citations

2
Genome-wide analysis of the SWEET gene family in Hemerocallis citrina and functional characterization of HcSWEET4a in response to salt stress DOI Creative Commons
Lihong Cao, Jinyao Wang, Lixuan Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Sugars will be eventually effluxed transporter (SWEET), one of the sugar transporters in plants, plays significant roles numerous biological processes and responding to abiotic stressors. However, characteristics functions SWEET genes Hemerocallis citrina remain unclear poorly elucidated. In this study, whole genome was utilized conduct bioinformatics analysis a total 19 HcSWEET were successfully identified. Analysis physicochemical properties indicated dominant differences among these HcSWEETs. A phylogenetic revealed that HcSWEET proteins can divided into 4 clades ranging from Clade I IV, where within same clade exhibited shared conserved motifs gene structures. Five six exons contained majority genes, which unevenly distributed across 11 chromosomes. The duplication showed presence pairs. Comparative syntenic maps family might present more closed homology monocotyledons than dicotyledons. Cis-acting element key responsiveness various hormones, light, stresses. Additionally, transcriptome sequencing suggested most had relatively higher expression roots, HcSWEET4a significantly up-regulated under salt stress. Overexpression further verified possibility HcSWEET4a is involved response stress, provides novel insights facilitates in-depth studies functional HcSWEETs resistance

Language: Английский

Citations

0
Glycine betaine alleviates low temperature combined with low light stress in pepper (Capsicum annuum L.) by improving photosynthetic ability and regulating genes related m6A methylases DOI

Nenghui Li,

Kaiguo Pu,

Miao Zhang

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 338, P. 113767 - 113767

Published: Oct. 30, 2024

Language: Английский

Citations

0
Genome-wide analysis of DnaJA proteins in Nicotiana tabacum reveals that NtDnaJA3 responses to drought stress DOI Creative Commons
Qiao Wang,

Lijun Meng,

Zechao Qu

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Abstract Background DnaJA proteins, a prominent subfamily of the DnaJ family, function as molecular chaperones that respond to various external stresses. Extensive studies on family have been conducted in plants. However, research this tobacco remains relatively scarce. Results In study, we identified 24 genes tobacco, and classified them into three individual groups. A comprehensive analysis based gene structure, motif composition, evolutionary pattern revealed divergence genes. For evolution NtDnaJA genes, purification selection was major factor. addition, potential regulatory network unveiled NtDnaJAs could be regulated by miRNAs transcription factors associated with diverse stress responses. Through expression qRT-PCR experiments, it observed many NtDnaJAs displayed tissue-specific might play significant roles different biotic abiotic Additionally, pivotal role NtDnaJA3 boosting plant drought resistance confirmed. Conclusions This study provides important perspectives their involvement responses, laying groundwork for future tobacco. Graphical Abstract

Language: Английский

Citations

0