The GA2ox Gene Family in Solanum pennellii: Genome-Wide Identification and Expression Analysis Under Salinity Stresses

Genes, Journal Year: 2025, Volume and Issue: 16(2), P. 158 - 158

Published: Jan. 26, 2025

-GA 2-oxidases (GA2oxs), a class of enzymes, inhibit the biosynthesis bioactive gibberellins (GAs) in plants. The GA2 oxidase gene is crucial for regulating passivation process active GA and widely involved hormone signaling abiotic stress processes. -To examine potential effects on Solanum pennellii, one important stress-tolerance wild species tomato, systematic analysis was performed to study structure, phylogenetic tree, genomic locus, upstream cis-regulatory elements SpGA2ox genes. expression patterns family various tissues were analyzed basis published RNA-seq data, changes leaves seedlings detected under salinity treatment by real-time fluorescence quantitative PCR. -We identified nine genes S. pennellii. They located chromosomes 1, 2, 4, 7, 8, 10. clearly divided into three groups through relationship analysis, namely, five C19-GA2ox I, II, C20-GA2ox class. And cis-element provided understanding function growth, development, hormones, GA2ox different classes, SpGA2ox1 exhibited higher levels stem compared other tissues. all increased significantly salt decreased with GA3. With largest relative levels, SpGA2ox3 SpGA2ox8 might exert key regulation synthesis response stress. -The present may be instrumental further investigation impact SpGA2oxs responses provide targets genetic improvement

Language: Английский

Integrative physiological and transcriptomic analysis provides insights on the molecular basis of ABA-enhanced drought tolerance in pear (Pyrus betulaefolia)

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 21, 2025

Drought stress could suppress the carbon assimilation and limit nutrient uptake of pear plants, thus affecting their growth severely impacting quality yield fruit. ABA is a hormone reported to alleviate drought in numerous plants. However, whether how functions responses plants yet explored. Here, address this gap, seedlings (Pyrus betulaefolia) were used subjected PEG-induced conditions with or without additional various doses. The results showed that while caused severe leaf water loss damage, applying at 50 µM 100 dramatically amended phenomenon, as indicated by markedly increased relative content, notably decreased electrolyte leakage malondialdehyde content. Based on RNA sequencing related physiological indices, it was found grossly disrupted chlorophyll synthesis photosynthesis. It induced over-production reactive oxygen species (ROS) broke ROS homeostasis, despite pronounced increases biosynthesis/content signaling, flavonoid synthesis, antioxidant enzyme activities, well sugar metabolism. applications significantly elevated expressions genes photosynthesis, partially boosting SPAD Fv/Fm values. In addition, treatments further prominently accelerate processes ABA, flavonoids, enzymes up-regulating corresponding genes, resulting endogenous accumulation enzymatic activity improvement, thereby expediting scavenging. Of course, metabolism pathway also outstandingly enhanced balance response seedlings. Moreover, through WGCNA analysis, core turquoise module associated ABA-attenuated identified, portion key transcription factors (TFs) some hub characterized, particularly for ERF, WRKY, MYB, bHLH, NAC TFs, CSP, COR, DHN genes. Overall, our study reveals exogenous help efficiently scavenge drought-induced improving photosynthesis capacity, accumulation, catabolism, system, etc. These will provide scientific basis practical direction utilizing mitigate adverse effects starvation from persistent high temperature summer. application ameliorated damage seedlings, working routes are reinforcement photosystem, biosynthesis metabolism, activities relevant regulatory network complex, primarily concerned focus potential target named DHN.

Language: Английский

Over Expression of Mango MiGA2ox12 in Tobacco Reduced Plant Height by Reducing GA1 and GA4 Content

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12109 - 12109

Published: Nov. 11, 2024

The regulation of gibberellic acid 2-oxidase (

Language: Английский