Development and Validation of a Near Infra-Red (NIR) Hand-held Spectrophotometric Method Using PCA Approaches and Chemometric Tools: Application for Qualitative and Quantitative Determination of Tadalafil Marketed in Kinshasa—D.R. Congo DOI Open Access
Jocelyn Mankulu Kakumba, Patient Ciza Hamuli,

Merdie Mpemba Luyinama

et al.

Spectroscopy Journal, Journal Year: 2024, Volume and Issue: 2(3), P. 105 - 122

Published: July 3, 2024

A hand-held NIR spectrophotometric method was developed, validated, and applied for the determination of tadalafil in tablets. The aim our work to develop analytical methods based on vibrational techniques using low-cost portable equipment. Based different chemometric modeling, we attempted validate method, which gave encouraging results from principal component analysis (PCA), DD-SIMCA, PLS modeling. Following this, optimized an appropriate experiment plan. For validation, used total error approach with acceptance limits set at ±10% a risk level 5%. showed that it possible perform both qualitative quantitative pharmaceutical products systems tools. developed enabled completion first step implementing quality control tadalafil-based drugs DRC. Validation difficulties resulted lack information about inter-day serial variations spectral responses. It would be interesting extend study larger calibration interval order correct uncertainties may result variability observed under conditions verify robustness. These are limitations this work, but nevertheless very encouraging.

Language: Английский

Integrative study of Anchusa italica Retz flower’s microstructure and chemical composition DOI Creative Commons
Linyang Wang,

Haiyan Xu,

Zenghong Sun

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 21, 2025

Language: Английский

Citations

0
Comparison of phenotypic and phytochemical profiles of 20 Lycium barbarum L. goji berry varieties during hot air-drying DOI Creative Commons
Ting Huang, Ning Jia,

Lunxuan Zhu

et al.

Food Chemistry X, Journal Year: 2025, Volume and Issue: 27, P. 102436 - 102436

Published: April 1, 2025

The variety and drying stage of goji berries (GBs) may affect their final physical chemical properties. This study investigated the differences in phenotypic parameters major composition 20 GBs varieties during four stages hot air-drying (HD). results indicated that color difference values L*, a*, b* decreased HD process. contents all amino acids decreased, with significant reductions involved Maillard reaction. Correspondingly, level 5-hydroxymethylfurfural, a reaction intermediates, increased. Furthermore, L* were closely linked to decomposition carotenoids. Notably, constituents among different dried smaller than those fresh GBs. These findings provide theoretical basis for optimization process, contributing expansion breeding programs use global functional food pharmaceutical industries.

Language: Английский

Citations

0
Widely Targeted Metabolomics Reveals the Bioactive Metabolites and Antioxidant Activities of Chinese Yam (Dioscorea opposita Thunb.) Peel DOI

Lan-Ping Yang,

Wendi Wang, Zhenzhen Liu

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: April 10, 2025

ABSTRACT Chinese yam ( Dioscorea opposita Thunb. cv. Tiegun) has been utilized in traditional medicine and as a food source for centuries. However, the metabolite profiles antioxidant activities of by‐product peel have not studied sufficiently. Thus, to effectively identify active metabolites peel, we employed UHPLC–MS/MS‐based widely targeted metabolomics on from loessial soil (LPCY) sandy (SPCY). A total 1054 were identified, comprising 379 primary metabolites, 528 secondary 147 other compounds. Notably, multivariate analyses revealed presence 143 differentially accumulated (DAMs) between SPCY LPCY. Linoleic acid metabolism, phenylpropanoid biosynthesis, plant hormone signal transduction, pyruvate sphingolipid metabolism main regulated pathways. The DPPH, ABTS, FRAP assays demonstrated that LPCY significantly higher than those SPCY. Correlation analysis most DAMs, including phenolic acids, lipids, organic amino exhibited significant positive correlations with r ≥ 0.7, p < 0.05). These results indicate promotes accumulation antioxidant‐active Overall, this study suggests peels hold potential rich natural bioactive substances.

Language: Английский

Citations

0
Development and Validation of a Near Infra-Red (NIR) Hand-held Spectrophotometric Method Using PCA Approaches and Chemometric Tools: Application for Qualitative and Quantitative Determination of Tadalafil Marketed in Kinshasa—D.R. Congo DOI Open Access
Jocelyn Mankulu Kakumba, Patient Ciza Hamuli,

Merdie Mpemba Luyinama

et al.

Spectroscopy Journal, Journal Year: 2024, Volume and Issue: 2(3), P. 105 - 122

Published: July 3, 2024

A hand-held NIR spectrophotometric method was developed, validated, and applied for the determination of tadalafil in tablets. The aim our work to develop analytical methods based on vibrational techniques using low-cost portable equipment. Based different chemometric modeling, we attempted validate method, which gave encouraging results from principal component analysis (PCA), DD-SIMCA, PLS modeling. Following this, optimized an appropriate experiment plan. For validation, used total error approach with acceptance limits set at ±10% a risk level 5%. showed that it possible perform both qualitative quantitative pharmaceutical products systems tools. developed enabled completion first step implementing quality control tadalafil-based drugs DRC. Validation difficulties resulted lack information about inter-day serial variations spectral responses. It would be interesting extend study larger calibration interval order correct uncertainties may result variability observed under conditions verify robustness. These are limitations this work, but nevertheless very encouraging.

Language: Английский

Citations

0