Enhancing plant resistance to tobacco mosaic virus through the combined application of Verticillium dahliae Aspf2‐like protein and microelements DOI
Shichen Li,

Fupeng Mei,

Waqar Ahmed

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract BACKGROUND Tobacco mosaic virus (TMV) poses a significant threat to global agriculture, infecting economically vital crops such as tobacco, tomato, pepper, and potato. Previous studies have suggested that the Verticillium dahliae Aspf2‐like protein (VDAL) enhances plant resistance TMV. This study investigated preventive therapeutic effects of VDAL, with without microelements, on TMV by analyzing hormone levels, defense related enzyme activities, transcriptomic responses. RESULTS Plants were subjected six experimental treatments: CK0 (untreated control, no or VDAL treatment), CK (TMV inoculated control), T1 (preventive T2 + microelements), CT1 (therapeutic CT2 microelements). inoculation (CK) significantly increased ( P < 0.05) content, jasmonic acid (JA), salicylic (SA) activities enzymes, including benzoic 2‐hydroxylase (BA2H), peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD), compared CK0. Both treatments (T1 T2) effectively reduced content enhanced JA, SA, activities. Notably, microelement‐supplemented treatment (T2) showed 37.73% greater reduction in T1. Similarly, applications, 32.50% than CT1. Treatments also contents JA 5.48% 2.88%, respectively their respective controls. Transcriptomic analysis revealed these activated plant–pathogen interaction pathways pathogen‐associated molecular pattern‐triggered immunity PTI ), upregulation key genes (e.g., CALM , BAK1 PTI6 WRKY33 indicating robust antiviral response. CONCLUSION Overall, we conclude synergistic application microelements through coordinated activation phytohormone signaling, immune‐related gene expression. combined approach offers an effective, eco‐friendly alternative for sustainable management viral diseases agricultural crops. © 2025 Society Chemical Industry.

Language: Английский

Enhancing plant resistance to tobacco mosaic virus through the combined application of Verticillium dahliae Aspf2‐like protein and microelements DOI
Shichen Li,

Fupeng Mei,

Waqar Ahmed

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract BACKGROUND Tobacco mosaic virus (TMV) poses a significant threat to global agriculture, infecting economically vital crops such as tobacco, tomato, pepper, and potato. Previous studies have suggested that the Verticillium dahliae Aspf2‐like protein (VDAL) enhances plant resistance TMV. This study investigated preventive therapeutic effects of VDAL, with without microelements, on TMV by analyzing hormone levels, defense related enzyme activities, transcriptomic responses. RESULTS Plants were subjected six experimental treatments: CK0 (untreated control, no or VDAL treatment), CK (TMV inoculated control), T1 (preventive T2 + microelements), CT1 (therapeutic CT2 microelements). inoculation (CK) significantly increased ( P < 0.05) content, jasmonic acid (JA), salicylic (SA) activities enzymes, including benzoic 2‐hydroxylase (BA2H), peroxidase (POD), polyphenol oxidase (PPO), superoxide dismutase (SOD), compared CK0. Both treatments (T1 T2) effectively reduced content enhanced JA, SA, activities. Notably, microelement‐supplemented treatment (T2) showed 37.73% greater reduction in T1. Similarly, applications, 32.50% than CT1. Treatments also contents JA 5.48% 2.88%, respectively their respective controls. Transcriptomic analysis revealed these activated plant–pathogen interaction pathways pathogen‐associated molecular pattern‐triggered immunity PTI ), upregulation key genes (e.g., CALM , BAK1 PTI6 WRKY33 indicating robust antiviral response. CONCLUSION Overall, we conclude synergistic application microelements through coordinated activation phytohormone signaling, immune‐related gene expression. combined approach offers an effective, eco‐friendly alternative for sustainable management viral diseases agricultural crops. © 2025 Society Chemical Industry.

Language: Английский

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