A Validated Trigonelline-Based Method for the Standardization and Quality Control of Trigonella foenum-graecum L. DOI Creative Commons
Mohammed Aldholmi, Rizwan Ahmad, Salma Hago

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 1350 - 1350

Published: Dec. 4, 2024

Background Fenugreek, or Trigonella foenum-graecum L, is an edible and medicinal plant of the Fabaceae family. Fenugreek seeds are rich in proteins, lipids, essential nutrients, they also contain various phytochemicals, including flavonoids, steroidal saponins, coumarin, alkaloids such as trigonelline. Trigonelline (TG) a bioactive alkaloid initially extracted from fenugreek seeds. A substantial portion fenugreek’s health benefits may rely on presence TG. This study addresses critical need for fast, green, economical method that overcomes inefficiencies, high solvent usage, sensitivity limitations quantification Methods origins were using three green solvents: acetone (ACt), ethanol (EtOH), water (H2O). The UPLC-MS/MS was developed validated mobile phase H2O: EtOH, r2-value 0.999 linearity range 0.1-500 ppb adopted. with accuracy 98.6% trace analysis TG small amount (10 mg) samples five different origins. Results average extract yield 5.36 mg/100 mg standard deviation (SD) 6.3, highest observed H2O. ESI (+ve) resulted fragmentation pattern (m/z) 138→94.10→92.05→78.20. revealed concentration 181.4 (SD = 176.4), H2O (mean 392.7, SD 132.4 ppb), followed by EtOH 91.9, 83.3 ppb) ACt 59.5, 30.9 ppb). validation step substantiated efficiency reproducibility method. Conclusions be used effective tool rapid, economical, eco-friendly extraction diverse matrices pharmaceutical, cosmeceutical, herbal, food products.

Language: Английский

Comprehensive Ethnopharmacological Analysis of Medicinal Plants in the UAE: Lawsonia inermis, Nigella sativa, Ziziphus spina-christi, Allium cepa, Allium sativum, Cymbopogon schoenanthus, Matricaria aurea, Phoenix dactylifera, Portulaca oleracea, Reichardia tingitana, Salvadora persica, Solanum lycopersicum, Trigonella foenum-graecum, Withania somnifera, and Ziziphus lotus DOI Open Access

Rola Almasri,

Alaa S. Bedir,

Seham M. Al Raish

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(3), P. 411 - 411

Published: Jan. 23, 2025

The United Arab Emirates (UAE) is home to diverse indigenous medicinal plants traditionally used for centuries. This study systematically evaluates the pharmacological and nutritional potential of key plants, including Lawsonia inermis, Nigella sativa, Ziziphus spina-christi, Allium cepa, sativum, Cymbopogon schoenanthus, Matricaria aurea, Phoenix dactylifera, Portulaca oleracea, Reichardia tingitana, Salvadora persica, Solanum lycopersicum, Trigonella foenum-graecum, Withania somnifera, lotus. Comprehensive literature searches were conducted using PubMed, Scopus, Web Science identify studies relevant their uses. findings highlight therapeutic roles these in managing global health challenges such as gastrointestinal diseases, antimicrobial resistance through bioactive compounds like flavonoids, polyphenols, antioxidants. Additionally, contributions nutrition, essential vitamins minerals, are emphasized disease prevention promotion. While this research focuses on UAE, implications globally relevant, many also found traditional medicine across Asia, Africa, Europe. Integrating into healthcare systems offers solutions pressing public concerns, reduces reliance synthetic pharmaceuticals, promotes sustainable practices. work a valuable reference researchers, professionals, policymakers, bridging knowledge modern scientific applications globally.

Language: Английский

Citations

1
Trigonella foenum-graecum L. protects against renal function decline in a mouse model of type 2 diabetic nephropathy by modulating the PI3K-Akt-ERK signaling pathway DOI Creative Commons

Yang Niu,

Hongjuan Niu, Liqun Chi

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

Trigonella foenum-graecum L. (HLB) exhibits promising pharmacological properties for the treatment of type 2 diabetic nephropathy (DN). This study aims to enhance understanding HLB's pharmacodynamic effects and elucidate mechanisms underlying its therapeutic potential in DN. The HLB were initially evaluated a murine DN model through oral administration an aqueous extract HLB. primary bioactive constituents subsequently identified using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Network pharmacology analysis was integrated these data uncover molecular targets Key renal metabolites profiled untargeted metabolomics, followed by metabolic pathway enrichment conducted MetaboAnalyst 6.0 platform, which facilitated identification relevant pathways modulates Finally, quantitative real-time polymerase chain reaction (QRT-PCR) Western blot (WB) techniques employed validate expression levels key genes proteins, thereby confirming Animal experiments indicated that significantly improved blood glucose regulation function while reducing oxidative stress abnormalities lipid metabolism mice. A total 34 compounds 159 as active components metabolomics revealed 61 critical metabolites, among PI3K-Akt-ERK signaling pathway-known be involved diabetes-was highlighted crucial pathway. QRT-PCR WB analyses demonstrated upregulated MAPK1, MAPK3, AKT1, PI3K. These results suggest may alleviate modulating metabolism. Its are likely mediated pathway, along upregulation PI3K expression. lays groundwork further investigations into action

Language: Английский

Citations

0
Characterization of bioactive compounds in fenugreek genotypes in varying environments: diosgenin, trigonelline, and 4-hydroxyisoleucine DOI Creative Commons
Furkan Çoban, Hakan Özer, Bilal Yılmaz

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: March 24, 2025

This study investigates the effects of irrigated and non-irrigated conditions on bioactive compound content in fenugreek ( Trigonella foenum-graecum ) across 31 diverse genotypes from various geographical regions. The was conducted at Atatürk University Research Extension Center, Türkiye (N 39°55’59.9”, E 41°14’10.6”, altitude 1789 m) during 2021 2022 growing seasons. levels diosgenin, trigonelline, 4-hydroxyisoleucine analyzed under were found to be significantly influenced by genotype, environment, their interaction (Genotype × Environment), with a highly significant effect observed p < 0.001 level. compounds included diosgenin (0.50-0.93%), trigonelline (5.22-13.65 mg g - ¹), (0.41-1.90%). Notably, such as Sivas/TR, Amasya/TR, Konya/TR Samsun/TR exhibited higher all conditions, while Spain, Malaysia, France, India showed irrigation. Variability observed, some showing stability different environmental conditions. A negative correlation between fenugreek. Furthermore, Principal Component Analysis (PCA) cluster analysis effective revealing genetic diversity, morphological differences, genotype adaptability. findings highlight potential for selecting superior breeding programs focused enhancing yields, especially varying irrigation research emphasizes critical role factors optimizing production health-benefiting

Language: Английский

Citations

0
A Validated Trigonelline-Based Method for the Standardization and Quality Control of Trigonella foenum-graecum L. DOI Creative Commons
Mohammed Aldholmi, Rizwan Ahmad, Salma Hago

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 1350 - 1350

Published: Dec. 18, 2024

Fenugreek, or Trigonella foenum-graecum L, is an edible and medicinal plant of the Fabaceae family. Fenugreek seeds are rich in proteins, lipids, essential nutrients, they also contain various phytochemicals, including flavonoids, steroidal saponins, coumarin, alkaloids such as trigonelline. Trigonelline (TG) a bioactive alkaloid initially extracted from fenugreek seeds. A substantial portion fenugreek's health benefits may rely on presence TG. This study addresses critical need for fast, green, economical method that overcomes inefficiencies, high solvent usage, sensitivity limitations quantification origins were using three green solvents: acetone (ACt), ethanol (EtOH), water (H 2O). The UPLC-MS/MS was developed validated mobile phase H 2O: EtOH, r 2-value 0.999 linearity range 0.1-500 ppb adopted. with accuracy 98.6% trace analysis TG small amount (10 mg) samples five different origins. average extract yield 5.36 mg/100 mg standard deviation (SD) 6.3, highest observed 2O. ESI (+ve) resulted fragmentation pattern ( m/z) 138→94.10→92.05→78.20. revealed concentration 181.4 (SD = 176.4), 2O (mean 392.7, SD 132.4 ppb), followed by EtOH 91.9, 83.3 ppb) ACt 59.5, 30.9 ppb). validation step substantiated efficiency reproducibility method. be used effective tool rapid, economical, eco-friendly extraction diverse matrices pharmaceutical, cosmeceutical, herbal, food products.

Language: Английский

Citations

1
A Validated Trigonelline-Based Method for the Standardization and Quality Control of Trigonella foenum-graecum L. DOI Creative Commons
Mohammed Aldholmi, Rizwan Ahmad, Salma Hago

et al.

F1000Research, Journal Year: 2024, Volume and Issue: 13, P. 1350 - 1350

Published: Dec. 4, 2024

Background Fenugreek, or Trigonella foenum-graecum L, is an edible and medicinal plant of the Fabaceae family. Fenugreek seeds are rich in proteins, lipids, essential nutrients, they also contain various phytochemicals, including flavonoids, steroidal saponins, coumarin, alkaloids such as trigonelline. Trigonelline (TG) a bioactive alkaloid initially extracted from fenugreek seeds. A substantial portion fenugreek’s health benefits may rely on presence TG. This study addresses critical need for fast, green, economical method that overcomes inefficiencies, high solvent usage, sensitivity limitations quantification Methods origins were using three green solvents: acetone (ACt), ethanol (EtOH), water (H2O). The UPLC-MS/MS was developed validated mobile phase H2O: EtOH, r2-value 0.999 linearity range 0.1-500 ppb adopted. with accuracy 98.6% trace analysis TG small amount (10 mg) samples five different origins. Results average extract yield 5.36 mg/100 mg standard deviation (SD) 6.3, highest observed H2O. ESI (+ve) resulted fragmentation pattern (m/z) 138→94.10→92.05→78.20. revealed concentration 181.4 (SD = 176.4), H2O (mean 392.7, SD 132.4 ppb), followed by EtOH 91.9, 83.3 ppb) ACt 59.5, 30.9 ppb). validation step substantiated efficiency reproducibility method. Conclusions be used effective tool rapid, economical, eco-friendly extraction diverse matrices pharmaceutical, cosmeceutical, herbal, food products.

Language: Английский

Citations

0