Harnessing de novo transcriptome sequencing to identify and characterize genes regulating carbohydrate biosynthesis pathways in Salvia guaranitica L. DOI Creative Commons
Zahid Khorshid Abbas, Arwa Abdulkreem AL‐Huqail,

Aesha H Abdel Kawy

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 26, 2024

Introduction Carbohydrate compounds serve multifaceted roles, from energy sources to stress protectants, found across diverse organisms including bacteria, fungi, and plants. Despite this broad importance, the molecular genetic framework underlying carbohydrate biosynthesis pathways, such as starch, sucrose, glycolysis/gluconeogenesis in Salvia guaranitica , remains largely unexplored. Methods In study, Illumina-HiSeq 2500 platform was used sequence transcripts of S. leaves, generating approximately 8.2 Gb raw data. After filtering removing adapter sequences, 38 million reads comprising 210 high-quality nucleotide bases were obtained. De novo assembly resulted 75,100 unigenes, which annotated establish a comprehensive database for investigating glycolysis biosynthesis. Functional analyses glucose-6-phosphate isomerase ( SgGPI ), trehalose-6-phosphate synthase/phosphatase SgT6PS sucrose synthase SgSUS ) performed using transgenic Arabidopsis thaliana . Results Among 410 identified putatively involved these metabolic 175 related 235 starch Overexpression A. enhanced leaf area, accelerated flower formation, promoted overall growth compared wild-type Discussion These findings lay foundation understanding roles genes offering insights into future engineering strategies enhancing production valuable or other

Language: Английский

Genetic diversity and population structure of Turkish European chestnut (Castanea sativa) genotypes assessed using start codon targeted polymorphism (SCoT) markers DOI Creative Commons
Erdal Orman, Deniz Çakar, Mehtap Alkan

et al.

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

Citations

0
Gamma-Ray-Induced Genetic Variability for Yield Traits in M4 Generation in Upland Rice DOI Open Access

Habibata Tinta,

V.S.E. Traoré,

Minimassom Philippe Nikiéma

et al.

Agricultural Sciences, Journal Year: 2025, Volume and Issue: 16(02), P. 240 - 255

Published: Jan. 1, 2025

Language: Английский

Citations

0
Intraspecific seed competition and genetic diversity within the capsule fruits of Aristolochia contorta DOI Creative Commons
Si‐Hyun Park, Jae Geun Kim

Functional Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: March 22, 2025

Abstract Genetic diversity and competitive dynamics are crucial in shaping plant communities. However, these have not been examined thoroughly the context of seed position within a fruit. This study integrated genetic analysis competition experiments to understand Aristolochia contorta . By combining molecular markers trials, we aimed deduce roles factors influencing seedling success, facilitating comprehensive assessment both environmental contributions growth patterns this species. Centrally positioned seeds showed superior germination rates due their larger size weight, providing edge over peripheral seeds, highlighting influence on nutrient allocation during germination. Regarding fruit orientation sowing, vertically upward‐sown fruits highest number shoots per fruit, suggesting that physical may early growth. result emphasizes importance (such as light exposure soil contact) determining success early‐stage seedlings. was low among fruits, with differentiation also being groups. Most variation observed individuals rather than between groups, indicating relatively homogeneous structure population. The be attributed species' reproductive strategies, particularly efficiency pollinators, which restricts gene flow variation. In summary, results suggest non‐genetic factors, such conditions position, play significant role interactions. Our findings provide valuable insights into evolutionary biology conservation strategies A. highlights potential challenges for species adapting changes. remain robust despite small sampling use whole DNA extraction. They emphasize considering efforts. Read free Plain Language Summary article Journal blog.

Language: Английский

Citations

0
Stress-Responsive Gene Expression, Metabolic, Physiological, and Agronomic Responses by Consortium Nano-Silica with Trichoderma against Drought Stress in Bread Wheat DOI Open Access
Ghalia Saleem Aljeddani,

Ragaa A. Hamouda,

Amal M. Abdelsattar

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 10954 - 10954

Published: Oct. 11, 2024

The exploitation of drought is a critical worldwide challenge that influences wheat growth and productivity. This study aimed to investigate synergistic amendment strategy for using the single combined application plant growth-promoting microorganisms (PGPM) (Trichoderma harzianum) biogenic silica nanoparticles (SiO2NPs) from rice husk ash (RHA) on Saudi Arabia’s Spring Summit cultivar (Triticum aestivum L.) 102 DAS (days after sowing). significant improvement was due 600 ppm SiO2NPs T. harzianum + SiO2NPs, which enhanced physiological properties chlorophyll a, carotenoids, total pigments, osmolytes, antioxidant contents drought-stressed plants as adaptive strategies. results suggest expression studied genes (TaP5CS1, TaZFP34, TaWRKY1, TaMPK3, TaLEA, housekeeping gene TaActin) in remarkably tolerance stress. We discovered metabolites involved significantly contributed defense responses, making them potential targets assessing levels. indices were revealed by mean productivity (MP), stress sensitivity index (SSI), yield stability (YSI), (STI). employed four databases, such BAR, InterPro, phytozome, KEGG pathway, predict decipher putative domains prior sequencing. As result, we these may be range important biological functions specific tissues at different developmental stages, including response stress, proline accumulation, development, response. In conclusion, sole and/or dual improved strength. These findings could insightful data production Arabia under various water regimes.

Language: Английский

Citations

1
Genetic Diversity and Population Structure of Turkish European Chestnut (Castanea sativa) Genotypes Assessed Using Start Codon Targeted Polymorphism (SCoT) Markers DOI Creative Commons
Erdal Orman, Deniz Çakar, Mehtap Alkan

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 15, 2024

Abstract The European chestnut (Castanea sativa) is an important nut crop that grows naturally in the Black Sea and Aegean regions of Turkey. This study examined genetic diversity population structure genotypes from prominent Turkey using Start Codon Targeted Polymorphism (SCoT) markers. A total 44 Turkish Aegean, Marmara, regions, along with a control group French variety, were analyzed. SCoT primers underwent tests to select most suitable ones, producing 8 selected amplified fragments, 65.34% which found be polymorphic. UPGMA PCoA analyses showed clear discrimination between two populations based on their origins, was supported by analysis. AMOVA analysis revealed 3% variation within 97% among individuals. out-group (French variety) furthest similarity, similarity values decreased increasing geographic distance. successfully fingerprinted could used future studies analyze phylogeny chestnuts genomic DNA.

Language: Английский

Citations

0
Harnessing de novo transcriptome sequencing to identify and characterize genes regulating carbohydrate biosynthesis pathways in Salvia guaranitica L. DOI Creative Commons
Zahid Khorshid Abbas, Arwa Abdulkreem AL‐Huqail,

Aesha H Abdel Kawy

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 26, 2024

Introduction Carbohydrate compounds serve multifaceted roles, from energy sources to stress protectants, found across diverse organisms including bacteria, fungi, and plants. Despite this broad importance, the molecular genetic framework underlying carbohydrate biosynthesis pathways, such as starch, sucrose, glycolysis/gluconeogenesis in Salvia guaranitica , remains largely unexplored. Methods In study, Illumina-HiSeq 2500 platform was used sequence transcripts of S. leaves, generating approximately 8.2 Gb raw data. After filtering removing adapter sequences, 38 million reads comprising 210 high-quality nucleotide bases were obtained. De novo assembly resulted 75,100 unigenes, which annotated establish a comprehensive database for investigating glycolysis biosynthesis. Functional analyses glucose-6-phosphate isomerase ( SgGPI ), trehalose-6-phosphate synthase/phosphatase SgT6PS sucrose synthase SgSUS ) performed using transgenic Arabidopsis thaliana . Results Among 410 identified putatively involved these metabolic 175 related 235 starch Overexpression A. enhanced leaf area, accelerated flower formation, promoted overall growth compared wild-type Discussion These findings lay foundation understanding roles genes offering insights into future engineering strategies enhancing production valuable or other

Language: Английский

Citations

0