Genome-Wide Identification of LACS Family Genes and Functional Characterization of CaLACS6/9 in Response to Cold Stress in Pepper (Capsicum annuum L.) DOI Creative Commons
Jianwei Zhang,

Yue Chen,

Jing He

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 970 - 970

Published: April 17, 2025

Long-chain acyl-CoA synthetase (LACS) is a crucial enzyme involved in cellular lipid metabolism, playing significant role plant development and adaptation to environmental stress. However, our understanding of the CaLACS gene family pepper remains limited. In this study, we identified nine members ‘UCD-10X-F1’ genome named them CaLACS1-CaLACS9 based on their chromosomal distribution. Phylogenetic analysis revealed that subfamily I-A includes CaLACS1, CaLACS3, CaLACS7; I-C contains CaLACS2; II comprises CaLACS4 CaLACS8; III consists remaining members. Collinearity showed there were twelve collinear pairs between six genes five AtLACS genes, two fragment replication pepper. Furthermore, numerous cis-acting elements associated with stress response, hormonal regulation, development, light response promoter regions genes. RNA-seq indicated exhibit tissue specificity are widely expressed leaves following treatment exogenous hormones, under conditions cold, heat, drought, salt Additionally, virus-induced silencing (VIGS) technology was employed further investigate roles CaLACS6 CaLACS9. Silencing these target seedlings increased sensitivity cold stress, as evidenced by accumulation reactive oxygen species (ROS), reduced antioxidant defense capacity, decreased expression levels cold-responsive ROS-related The findings study provide valuable insights into functional highlight CaLACS9 promising candidate for enhancing tolerance

Language: Английский

Genome-Wide Identification of LACS Family Genes and Functional Characterization of CaLACS6/9 in Response to Cold Stress in Pepper (Capsicum annuum L.) DOI Creative Commons
Jianwei Zhang,

Yue Chen,

Jing He

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 970 - 970

Published: April 17, 2025

Long-chain acyl-CoA synthetase (LACS) is a crucial enzyme involved in cellular lipid metabolism, playing significant role plant development and adaptation to environmental stress. However, our understanding of the CaLACS gene family pepper remains limited. In this study, we identified nine members ‘UCD-10X-F1’ genome named them CaLACS1-CaLACS9 based on their chromosomal distribution. Phylogenetic analysis revealed that subfamily I-A includes CaLACS1, CaLACS3, CaLACS7; I-C contains CaLACS2; II comprises CaLACS4 CaLACS8; III consists remaining members. Collinearity showed there were twelve collinear pairs between six genes five AtLACS genes, two fragment replication pepper. Furthermore, numerous cis-acting elements associated with stress response, hormonal regulation, development, light response promoter regions genes. RNA-seq indicated exhibit tissue specificity are widely expressed leaves following treatment exogenous hormones, under conditions cold, heat, drought, salt Additionally, virus-induced silencing (VIGS) technology was employed further investigate roles CaLACS6 CaLACS9. Silencing these target seedlings increased sensitivity cold stress, as evidenced by accumulation reactive oxygen species (ROS), reduced antioxidant defense capacity, decreased expression levels cold-responsive ROS-related The findings study provide valuable insights into functional highlight CaLACS9 promising candidate for enhancing tolerance

Language: Английский

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