Hyperbaric Oxygen Promotes Chronic Wound Healing in Sprague-Dawley Rats by Inhibiting Pyroptosis DOI Creative Commons
Hongxia Ma, Xing Wei, Ethan Lin

et al.

Physiological Research, Journal Year: 2024, Volume and Issue: unknown, P. 1049 - 1061

Published: Dec. 31, 2024

This study aimed to establish a rat model of chronic wounds observe the effects hyperbaric oxygen (HBO) on wound repair and pyroptosis explore potential role in pathogenesis wounds. Sprague-Dawley (SD) rats were randomly divided into acute group (control group), (model + HBO treatment (HBO VX-765 (IL-converting enzyme/Caspase-1 inhibitor) (VX-765 group). After 7 days respective interventions, healing status was observed, tissue specimens collected. Hematoxylin eosin (HE) staining used pathological changes tissues. Transmission electron microscopy cellular ultrastructure. Immunofluorescence expression localization vascular endothelial growth factor A (VEGF-A) N-terminal domain gasdermin D (GSDMD-N). Western blot conducted detect nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteine-requiring aspartate protease-1 (Caspase-1), VEGF-A, GSDMD-N proteins Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) NLRP3, Caspase-1, GSDMD genes. Enzyme-linked immunosorbent assay (ELISA) performed inflammatory cytokines interleukin-1 beta (IL-1β) IL-18. The results showed that had faster rate better pathology improvement compared group. level VEGF-A higher group, while levels GSDMD, IL-1β, IL-18 lower than those can effectively promote wounds, regulation may be one its mechanisms action. Keywords: Hyperbaric • Pyroptosis Chronic Inflammatory

Language: Английский

Pathological Insights into Cell Death Pathways in Diabetic Wound Healing DOI
Kannan Harithpriya,

Srinivasan Kaussikaa,

Srikanth Kavyashree

et al.

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 264, P. 155715 - 155715

Published: Nov. 7, 2024

Language: Английский

Citations

4
Role of brain-gut axis in modulating stress responses via neurotransmitters and cytokines in largemouth bass (Micropterus salmoides) under acute crowding stress DOI

Leshan Yang,

Meijia Li, Zhang Jia

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742502 - 742502

Published: March 1, 2025

Language: Английский

Citations

0
Tongfu Xingshen Capsule Alleviates Stroke-Associated Pneumonia-Induced Multiple Organ Injuries by Modulating the Gut Microbiota and Sphingolipid Metabolism DOI
Ruihua Wang,

Changlian Gan,

Baoying Gong

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: 142, P. 156756 - 156756

Published: April 12, 2025

Language: Английский

Citations

0
A gut check: understanding the interplay of the gastrointestinal microbiome and the developing immune system towards the goal of pediatric HIV remission DOI Creative Commons

Nicole Soo,

Omotayo Farinre,

Ann Chahroudi

et al.

Retrovirology, Journal Year: 2024, Volume and Issue: 21(1)

Published: Oct. 18, 2024

Abstract Despite the efficacy of antiretroviral therapy (ART) in reducing global incidence vertical HIV transmissions, more than 120,000 children are still infected with virus each year. Since ART cannot clear reservoir that is established soon after infection, living (CLWH) forced to rely on for their lives and suffer from long-term drug-related complications. Pediatric like adult associated gut microbial dysbiosis, loss epithelial integrity, bacterial translocation, CD4 + T cell depletion, systemic immune activation, viral establishment. However, unlike adults, vertically acquired by infants interacts a microbiome continuously evolving while concomitantly shaping infant’s ontogeny. Therefore, determine whether there may be interventions target through microbiome-directed approaches, understanding complex tripartite interactions between transmitted HIV, maturing microbiome, developing system during early life crucial. Importantly, time when an individual highly dynamic, this temporal development plays crucial role educating child. manipulation CLWH phenotype can reduce persistence fostering antiviral might opportune strategy achieve ART-free suppression CLWH. This review summarizes current state knowledge transmission CLWH, landscape pediatric elite controllers, explores prospect employing modulation as potential therapeutic approach population.

Language: Английский

Citations

1
Hyperbaric Oxygen Promotes Chronic Wound Healing in Sprague-Dawley Rats by Inhibiting Pyroptosis DOI Creative Commons
Hongxia Ma, Xing Wei, Ethan Lin

et al.

Physiological Research, Journal Year: 2024, Volume and Issue: unknown, P. 1049 - 1061

Published: Dec. 31, 2024

This study aimed to establish a rat model of chronic wounds observe the effects hyperbaric oxygen (HBO) on wound repair and pyroptosis explore potential role in pathogenesis wounds. Sprague-Dawley (SD) rats were randomly divided into acute group (control group), (model + HBO treatment (HBO VX-765 (IL-converting enzyme/Caspase-1 inhibitor) (VX-765 group). After 7 days respective interventions, healing status was observed, tissue specimens collected. Hematoxylin eosin (HE) staining used pathological changes tissues. Transmission electron microscopy cellular ultrastructure. Immunofluorescence expression localization vascular endothelial growth factor A (VEGF-A) N-terminal domain gasdermin D (GSDMD-N). Western blot conducted detect nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), cysteine-requiring aspartate protease-1 (Caspase-1), VEGF-A, GSDMD-N proteins Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) NLRP3, Caspase-1, GSDMD genes. Enzyme-linked immunosorbent assay (ELISA) performed inflammatory cytokines interleukin-1 beta (IL-1β) IL-18. The results showed that had faster rate better pathology improvement compared group. level VEGF-A higher group, while levels GSDMD, IL-1β, IL-18 lower than those can effectively promote wounds, regulation may be one its mechanisms action. Keywords: Hyperbaric • Pyroptosis Chronic Inflammatory

Language: Английский

Citations

0