High rate of false positive malaria rapid diagnostic tests in a district hospital in Uganda DOI Creative Commons

Susanne E. Mortazavi,

Allan Lugaajju,

Anna-Clara Ivarsson

et al.

Frontiers in Malaria, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 31, 2025

Background Malaria remains a significant health burden, particularly in Uganda, which has one of the highest incidence rates globally. Rapid diagnostic tests (RDTs) are widely used for malaria diagnosis due to their ease use and affordability resource-limited settings. However, false-positive RDT results may lead unnecessary antimalarial treatments missed diagnoses other febrile conditions. This study aimed evaluate accuracy compared microscopy loop-mediated isothermal amplification (LAMP) malaria-endemic region Uganda. Methods A prospective was conducted with 225 individuals diagnosed using RightSign Ag HRPII/Pan Plasmodium Aldolase (Hangzhou Biotest Biotech Co., Ltd., China) at Iganga General Hospital, Blood samples were analyzed LAMP. Demographic information, history, parasitemia levels also recorded. Results Among RDT-positive participants, 48% tested negative by microscopy, 45% these LAMP, indicating that 20% all likely false positives. Overall, 66% (149/225) cases confirmed as true Conclusions identified high rate positives RDT, risking inappropriate treatment illnesses. It is essential healthcare facilities RDTs validated recommended World Health Organization (WHO) ensure specificity accuracy, settings where alternative methods not be available.

Language: Английский

High rate of false positive malaria rapid diagnostic tests in a district hospital in Uganda DOI Creative Commons

Susanne E. Mortazavi,

Allan Lugaajju,

Anna-Clara Ivarsson

et al.

Frontiers in Malaria, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 31, 2025

Background Malaria remains a significant health burden, particularly in Uganda, which has one of the highest incidence rates globally. Rapid diagnostic tests (RDTs) are widely used for malaria diagnosis due to their ease use and affordability resource-limited settings. However, false-positive RDT results may lead unnecessary antimalarial treatments missed diagnoses other febrile conditions. This study aimed evaluate accuracy compared microscopy loop-mediated isothermal amplification (LAMP) malaria-endemic region Uganda. Methods A prospective was conducted with 225 individuals diagnosed using RightSign Ag HRPII/Pan Plasmodium Aldolase (Hangzhou Biotest Biotech Co., Ltd., China) at Iganga General Hospital, Blood samples were analyzed LAMP. Demographic information, history, parasitemia levels also recorded. Results Among RDT-positive participants, 48% tested negative by microscopy, 45% these LAMP, indicating that 20% all likely false positives. Overall, 66% (149/225) cases confirmed as true Conclusions identified high rate positives RDT, risking inappropriate treatment illnesses. It is essential healthcare facilities RDTs validated recommended World Health Organization (WHO) ensure specificity accuracy, settings where alternative methods not be available.

Language: Английский

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