Efficacy and Safety of Stem-Cell Therapy for Acute and Subacute Ischemic Stroke: Improving Long-Term Outcomes - A Systematic Review and Meta-Analysis DOI
Toshiya Osanai, Soichiro Takamiya, Yasuhiro Morii

et al.

Published: Jan. 1, 2024

Language: Английский

Comparative Assessment of Manual Segmentation of Cerebral Infarction Lesions in Experimental Animals Based on Magnetic Resonance Imaging Using Artificial Intelligence DOI

I. L. Gubskiy,

Daria D. Namestnikova, Elvira Cherkashova

et al.

Bulletin of Experimental Biology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

Language: Английский

Citations

0
Human mesenchymal stem cell expansion on laminin-521 in serum-free and xeno-free culture conditions DOI Creative Commons
Halima Albalushi,

Mohadese Boorojerdi,

Elias A. Said

et al.

Journal of Applied Biomaterials & Functional Materials, Journal Year: 2025, Volume and Issue: 23

Published: April 1, 2025

Laminin-521 (LN521) is a crucial adhesion protein found in natural stem cell niches and plays an important role maintaining human pluripotent (PSC) properties. This study aimed to investigate the effects of LN521 on umbilical cord-derived mesenchymal (UC-MSC) characteristics Serum-free Xeno-free culture conditions as step toward clinical application. In our experiment isolated UC-MSC via explant method were expanded homogeneous monolayer morphologically, presented typical MSC-like morphology (spindle-shaped) from passage three six when cultured either or CELLstart™. Almost, 90% confluency was reached after 4 days with EI approximately 11.2 no statistically significant differences CELLstart™ all passages. Phenotypic characterization using flow cytometry, along expression same biomarkers gene level analyzed by quantitative reversed transcription revealed identical CD73, CD90, CD105, CD34, CD45, CD19, CD14, HLA-DR pattern at passages both Moreover, presence showed adipogenesis, chondrogenesis osteogenesis differentiation potential, normal chromosome structure highlighting genetic stability. Ultimately, comparable supporting expansion their serum-free xeno-free conditions.

Language: Английский

Citations

0
Tissue Engineering and Regenerative Medicine: Perspectives and Challenges DOI Creative Commons
Van T. Hoang, Quyen Thi Nguyen,

Trang Thi Kieu Phan

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(5)

Published: April 24, 2025

ABSTRACT From the pioneering days of cell therapy to achievement bioprinting organs, tissue engineering, and regenerative medicine have seen tremendous technological advancements, offering solutions for restoring damaged tissues organs. However, only a few products technologies received United States Food Drug Administration approval. This review highlights significant progress in therapy, extracellular vesicle‐based engineering. Hematopoietic stem transplantation is powerful tool treating many diseases, especially hematological malignancies. Mesenchymal cells been extensively studied. The discovery induced pluripotent has revolutionized disease modeling applications, paving way personalized medicine. Gene represents an innovative approach treatment genetic disorders. Additionally, therapies emerged as rising stars, promising diagnostics, cell‐free therapeutics, drug delivery, targeted therapy. Advances engineering enable complex constructs, further transforming field. Despite these technical, ethical, regulatory challenges remain. addresses current bottlenecks, emphasizing novel interdisciplinary research overcome hurdles. Standardizing practices conducting clinical trials will balance innovation regulation, improving patient outcomes quality life.

Language: Английский

Citations

0
Efficacy and Safety of Stem-Cell Therapy for Acute and Subacute Ischemic Stroke: Improving Long-Term Outcomes - A Systematic Review and Meta-Analysis DOI
Toshiya Osanai, Soichiro Takamiya, Yasuhiro Morii

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0