Concordance of maternal and cord blood SARS-COV-2 immunoglobulin seropositivity after COVID-19 infection or vaccination in pregnancy

Journal of Neonatal-Perinatal Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Objective To assess maternal antibody response to Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection during pregnancy and subsequent transplacental transfer in cord blood. Study Design This is a prospective cohort study of Disease 2019 (COVID-19) polymerase chain reaction (PCR) positive pregnant women their newborns. SARS-CoV-2 PCR (+) were enrolled, with (−) as control. Maternal blood was obtained at enrollment collected delivery. Baseline infant characteristics neonatal outcomes collected. Samples analyzed using coronavirus antigen microarray containing immunologically significant antigens from (including nucleocapsid protein [NP], spike [S], S1, S2, receptor-binding domain [RBD]) which can detect immunoglobulin G (IgG) M (IgM). Results Thirty-seven maternal-cord paired samples for IgG or IgM antibodies; 15 out 20 14 17 mothers positive. the received COVID-19 vaccine pregnancy. Difference between seropositivity naturally infected versus vaccinated significant, 75% 100% ( p = 0.043). antibodies detected 10 but none Conclusions Excellent concordance exist Significantly higher SARS-COV-2 found mothers.

Language: Английский

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Factors modulating maternofetal transfer of IgG antibodies following SARS-CoV-2 gestational infection

Revista do Instituto de Medicina Tropical de São Paulo, Journal Year: 2025, Volume and Issue: 67

Published: Jan. 1, 2025

ABSTRACT Early infant immunity to SARS-CoV-2 depends on maternofetal transfer of antibodies. We aimed analyze the factors modulating anti-SARS-CoV-2 IgG antibodies following gestational infection during pandemic in Brazil (April–August 2021). conducted a retrospective and prospective cohort study involving 509 mother-child dyads tested simultaneously for anti-nucleocapsid universal neonatal screening. There were 341 seronegative 168 seropositive ones. Seropositive neonates retested two three months later. examined association serological status concentrations with mRNA vaccination, timing maternal infection, conditions, gender. Gestational predicted seropositivity (OR=3.97; 95%CI=2.69–5.88). Maternal first, second, or third trimester was associated progressively greater (34.4%, 51.6%, 58.2%, respectively; p=0.03). Among neonates, concentration higher when mothers reported they had COVID-19 pregnancy (p=0.04) tended be lower girls (p=0.06). More than half remained later (54.1%), which both at birth (p<0.001). Higher persistence anti-N more newborns. This provides an additional understanding dynamics antibody transfer.

Language: Английский

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Seroprevalence and placental transfer of SARS-CoV-2 antibodies in unvaccinated pregnant women

BMC Infectious Diseases, Journal Year: 2024, Volume and Issue: 24(1)

Published: May 21, 2024

Abstract Purpose Pregnant women are at risk of severe SARS-CoV-2 infection, potentially leading to obstetric and neonatal complications. Placental transfer antibodies directed may be protective against COVID-19, but this remains studied. We aimed determine the seroprevalence in a population unvaccinated pregnant placental these antibodies. Methodology A total 1197 with mostly unknown pre-study infection status, were tested delivery for spike protein IgG during first year pandemic. Umbilical cord samples collected assessed seropositivity if mother was seropositive. Maternal characteristics, pregnancy outcomes data on extracted from medical records. Results Specific detected 258 (21.6%). significant newborn observed 81.3% cases. The earlier 2nd 3rd trimesters that had contracted disease more symptomatic she was, greater likelihood transplacental her newborn. Conclusion Approximately one five detectable anti-SARS-CoV-2 pandemic, significantly transferred their fetuses. This research provides further evidence better understand dynamics mothers newborns, which is necessary improve vaccination strategies.

Language: Английский

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Acquisition of anti-phosphatidylserine IgM and IgG antibodies by infants and their mothers over time in Uganda

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 26, 2024

Background Production of anti-phosphatidylserine (anti-PS) antibodies has been associated with malaria and can aggravate pathology. How these autoantibodies develop during early childhood in a context is not known. We examined levels anti-PS IgG IgM longitudinal cohort mother-baby pairs birth, the infants at 2.5, 6 months, mothers their babies 9 months postpartum. Results There was no difference between cord blood mothers’ peripheral birth. However, were significantly higher compared to infants’ blood, steadily increasing first life. In that had highest there decline until rise months. Infants possessed high birth also exhibited progressive levels. When correlated different fractions B-cells, several correlations P. falciparum specific atypical B cells both 2.5 for infants, especially IgM. Anti-PS strongly C1q-fixing Conclusion These results show acquired by could be transferred transplacentally targeting PS are year have increased knowledge about autoimmune responses infections life critical comprehensive understanding vaccine functionality endemic areas.

Language: Английский

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Unveiling the Quest: Crafting an Enzyme-Linked Immunosorbent Assay (ELISA) Technique to Uncover COVID-19 Antibodies

Cureus, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 11, 2024

The COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, has had a profound impact on global health. Rapid and accurate diagnostic tools are crucial for effective disease control management. enzyme-linked immunosorbent assay (ELISA) emerged as reliable widely used method detecting antibodies in patients, which develop response to SARS-CoV-2 infection. While ELISA technique is identifying presence of thus confirming exposure virus, its role predicting clinical course severity limited. primarily confirms prior virus or vaccination status, but it does not directly correlate antibody levels with progression disease. variability outcomes influenced factors such viral load, patient co-morbidities, genetic predispositions, timing immune response. diverse applications epidemiology, assessment, therapeutic development. It determines prevalence, aids surveillance, evaluates vaccine effectiveness protection duration. quantitatively measures levels, providing insights into treatment efficacy. Challenges include specialized facilities personnel, cross-reactivity, false results. Multiplex assays integration other diagnostics future directions. In summary, an essential tool diagnostics, enabling precise assessment contributing strategies. development point-of-care devices that integrate technology could enable rapid accessible testing various settings. Additionally, integrating platforms enhance overall capabilities COVID-19. Despite challenges, ongoing advancements technology, approaches, hold promise further improving management

Language: Английский

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Neutralizing and binding antibody responses to SARS-CoV-2 with hybrid immunity in pregnancy

npj Vaccines, Journal Year: 2024, Volume and Issue: 9(1)

Published: Aug. 27, 2024

Abstract Hybrid immunity against SARS-CoV-2 has not been well studied in pregnancy. We conducted a comprehensive analysis of neutralizing antibodies (nAb) and binding pregnant individuals who received mRNA vaccination, natural infection, or both. A third vaccine dose augmented nAb levels compared to the two-dose regimen infection alone; this effect was more pronounced hybrid immunity. There reduced anti-Omicron nAb, but maternal-fetal transfer efficiency remained comparable that other variants. Vaccine-induced nAbs were transferred efficiently than infection-induced nAbs. Anti-spike receptor domain (RBD) IgG associated with wild-type (Wuhan-Hu-1) following breakthrough infection. Both vaccination anti-RBD IgA, which durable anti-nucleocapsid IgA. IgA response attenuated pregnancy non-pregnant controls. These data provide additional evidence augmentation humoral immune responses

Language: Английский

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