The comprehensive analysis of m6A-associated anoikis genes in low-grade gliomas DOI Creative Commons
Hui Zheng, Yutong Zhao,

Hai Zhou

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: June 8, 2023

Abstract Background The relationship between N6-methyladenosine (m6A) regulators and anoikis their effects on low-grade glioma (LGG) are not clear yet. Methods TCGA-LGG cohort, mRNAseq 325 dataset GSE16011 validation set were separately gained via Cancer Genome Atlas (TCGA), Chinese Glioma Altas (CGGA) Gene Expression Omnibus (GEO) databases. 27 m6A-related genes (m6A-RGs) 508 anoikis-related (ANRGs) extracted from published articles, individually. First, differentially expressed (DEGs) LGG Normal samples sifted out by differential expression analysis. DEGs respectively intersected with m6A-RGs ANRGs to acquire (DE-m6A-RGs) (DE-ANRGs). Correlation analysis of DE-m6A-RGs DE-ANRGs was performed obtain DE-m6A-ANRGs. Next, univariate Cox least absolute shrinkage selection operator (LASSO) DE-m6A-ANRGs sift risk model genes, score according them, then gene enrichment (GSEA) implemented based genes. After that, we constructed an independent prognostic model, immune infiltration drug sensitivity Finally, mRNA-miRNA-lncRNA regulatory network constructed. Results There 6901 samples. six 214 through intersecting ANRGs, respectively. 149 derived after correlation 4 namely ANXA5, KIF18A, BRCA1 HOXA10 composed the they involved in apoptosis, fatty acid metabolism glycolysis, etc.. age finally construct model. Activated CD4 T cell, gamma delta cell natural killer had largest positive while activated B significantly negatively correlated KIF18A BRCA1. AT.9283, EXEL.2280, Gilteritinib Pracinostat (Absolute value) score. (mRNAs)_, 12 miRNAs 21 lncRNAs formed network, containing HOXA10-hsa-miR-129-5p-LINC00689, KIF18A-hsa-miR- 221-3p-DANCR, Conclusion Through bioinformatics, a m6A-associated LGG, providing new ideas for research related prognosis treatment LGG.

Language: Английский

LncRNA-miRNA axis in tumor progression and therapy response: An emphasis on molecular interactions and therapeutic interventions DOI Open Access
Maliheh Entezari, Afshin Taheriazam,

Sima Orouei

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 154, P. 113609 - 113609

Published: Aug. 27, 2022

Epigenetic factors are critical regulators of biological and pathological mechanisms they could interact with different molecular pathways. Targeting epigenetic has been an idea approach in disease therapy, especially cancer. Accumulating evidence highlighted function long non-coding RNAs (lncRNAs) as cancer initiation development focused on their association downstream targets. microRNAs (miRNAs) the most well-known targets lncRNAs present review focuses lncRNA-miRNA axis malignancy therapy resistance tumors. LncRNA-miRNA regulates cell death such apoptosis autophagy cancers. This affects tumor metastasis via regulating EMT MMPs. Besides, determines sensitivity cells to chemotherapy, radiotherapy immunotherapy. Based studies, can be affected by drugs genetic tools this may affect expression level miRNAs targets, leading suppression/progression. LncRNAs have both tumor-promoting tumor-suppressor functions unique complicated implication therapy. also other signaling networks PI3K/Akt, STAT3, Wnt/β-catenin EZH2 among others. Notably, lncRNA/miRNA considered a signature for diagnosis prognosis

Language: Английский

Citations

55
Exosomal lncRNAs as diagnostic and therapeutic targets in multiple myeloma DOI Creative Commons
Hong Yan, Nan Jiang, M Kellis

et al.

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 16, 2025

Multiple Myeloma (MM) is the second most common malignancy of hematopoietic system, accounting for approximately 10% all hematological malignancies, and currently, there no complete cure. Existing research indicates that exosomal long non-coding RNAs (lncRNAs) play a crucial regulatory role in initiation progression tumors, involving various interactions such as lncRNA-miRNA, lncRNA-mRNA, lncRNA-RNA binding proteins (RBP). Despite significant clinical application potential lncRNAs, this area still faces challenges due to their low abundance technical limitations. To our knowledge, review first comprehensively integrate elucidate three mechanisms action lncRNAs MM, propose therapeutic targets cases based on these mechanisms. We highlight latest advancements biomarkers targets, offering not only comprehensive analysis MM but also new perspectives methods future diagnosis treatment multiple myeloma.

Language: Английский

Citations

1
Exploring the role of noncoding RNAs in cancer diagnosis, prognosis, and precision medicine DOI Creative Commons
Basmah Eldakhakhny,

Abdulaziz M. Sutaih,

Moaaz A. Siddiqui

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 9(4), P. 1315 - 1323

Published: July 1, 2024

This review article studies the complex field of noncoding RNAs (ncRNAs) in cancer biology, focusing on their potential use as biomarkers and therapeutic targets. NcRNAs include circular (circRNAs), long (lncRNAs), microRNAs (miRNAs). We discuss how ncRNAs affect gene expression cancerous cells, spread cancer, metastasis. The illustrates pathways through which can oncogenesis, tumor suppression, resistance. It also assesses clinical uses non-coding (ncRNAs), including utility diagnosis, prognosis. Besides, for personalized therapy is documented. Finally, it concluded that understanding role ncRNA importance regarding carcinogenesis, therapy.

Language: Английский

Citations

5
PSMA3-AS1 induced by transcription factor PAX5 promotes cholangiocarcinoma proliferation, migration and invasion by sponging miR-376a-3p to up-regulate LAMC1 DOI Creative Commons
Dongsheng Sun, Fujun Li, Lang Liu

et al.

Aging, Journal Year: 2022, Volume and Issue: 14(1), P. 509 - 525

Published: Jan. 12, 2022

Long noncoding RNAs (lncRNAs) have been reported to exhibit a crucial regulatory role in tumor progression, including cholangiocarcinoma (CCA). As promising lncRNA, proteasome 20S subunit alpha 3 antisense RNA 1 (PSMA3-AS1) is involved development of various tumors. However, the and function PSMA3-AS1 CCA remain unclear. The aim this study examine expression, function, mechanism, clinical significance development. By TCGA database analysis, we found that was overexpressed CCA. Consistent with significantly tissues cells by RT-qPCR. Upregulated related lymph node invasion, advanced TNM stage poor survival, an independent risk factor prognosis for patients. Functionally, CCK-8, EdU colony formation assays confirmed upregulated promoted cell proliferation, whereas downregulated inhibited proliferation. This result further subcutaneous nude mice. Wound healing transwell increased migration decreased these biological phenotypes. In addition, EMT process downregulating E-cadherin upregulating N-cadherin vimentin. Mechanistically, transcription PAX5 bound promoter region its transcription. Simultaneously, primarily localized cytoplasm could competitively bind miR-376a-3p upregulate LAMC1, thereby accelerating progression. uncovers functions as cancer-promoting gene CCA, PAX5/PSMA3-AS1/miR-376a-3p/LAMC1 axis plays vital

Language: Английский

Citations

22
Long noncoding RNA PSMA3-AS1 functions as a competing endogenous RNA to promote gastric cancer progression by regulating the miR-329-3p/ALDOA axis DOI Creative Commons
Liang Kan,

Meiqi Yang,

Huijing Zhang

et al.

Biology Direct, Journal Year: 2023, Volume and Issue: 18(1)

Published: July 4, 2023

Abstract LncRNA PSMA3-AS1 functions as an oncogene in several cancers, including ovarian cancer, lung and colorectal cancer. However, its role gastric cancer (GC) progression remains unclear. In this study, the levels of PSMA3-AS1, miR-329-3p, aldolase A (ALDOA) 20 paired human GC tissues adjacent nontumorous were measured by real-time PCR. cells transfected with recombinant plasmid carrying full-length or shRNA targeting PSMA3-AS1. The stable transfectants selected G418. Then, effects knockdown overexpression on vitro vivo evaluated. results showed that was highly expressed tissues. Stable significantly restrained proliferation/migration/invasion, enhanced cell apoptosis, induced oxidative stress vitro. Tumor growth matrix metalloproteinase expression tumor markedly inhibited, while nude mice after knockdown. Additionally, negatively regulated miR-329-3p positively ALDOA expression. MiR-329-3p directly targeted ALDOA-3′UTR. Interestingly, partially attenuated tumor-suppressive Conversely, exhibited opposite effects. promoted regulating miR-329-3p/ALDOA axis. might serve a promising effective target for treatment.

Language: Английский

Citations

10
Long Non-Coding RNAs in Malignant Human Brain Tumors: Driving Forces Behind Progression and Therapy DOI Open Access

Dakun Pei,

Dandan Zhang, Yan Guo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 694 - 694

Published: Jan. 15, 2025

Long non-coding RNAs (lncRNAs) play a pivotal role in regulating gene expression and are critically involved the progression of malignant brain tumors, including glioblastoma, medulloblastoma, meningioma. These lncRNAs interact with microRNAs (miRNAs), proteins, DNA, influencing key processes such as cell proliferation, migration, invasion. This review highlights multifaceted impact lncRNA dysregulation on tumor underscores their potential therapeutic targets to enhance efficacy chemotherapy, radiotherapy, immunotherapy. The insights provided offer new directions for advancing basic research clinical applications tumors.

Language: Английский

Citations

0
PSMA3-AS1: a promising LncRNA as a diagnostic and prognostic biomarker in human cancers DOI
Yunxia Niu, Junqing Meng, Xue Zhao

et al.

Gene, Journal Year: 2025, Volume and Issue: unknown, P. 149521 - 149521

Published: April 1, 2025

Language: Английский

Citations

0
Knockdown of miR-411-3p induces M2 macrophage polarization and promotes colorectal cancer progression by regulation of MMP7 DOI Creative Commons
Tianliang Bai, Ping Li, Yabin Liu

et al.

European Journal of Histochemistry, Journal Year: 2025, Volume and Issue: 69(2)

Published: May 5, 2025

Colorectal cancer (CRC) is prone to metastasis, leading a poor prognosis. miR-411-3p exhibits tumor-suppressive function in CRC, but its exact mechanism unclear. The malignant biological properties of CRC cells were detected by Carboxyfluorescein diacetate succinimidyl ester (CFSE) staining, scratch-wound and transwell assay. Levels markers associated with macrophage polarization evaluated flow cytometry ELISA kits. Bioinformatics analysis screen whether the downstream target mRNA matrix metalloproteinase 7 (MMP7), Dual-Luciferase reporter assay verified targeting relationship between two. qRT-PCR tested MMP7 levels. level was quantified Western blot. Additionally, nude mouse subcutaneous graft tumor model constructed, Ki-67 expression immunohistochemistry, impact miR-411-3p/MMP7 on M2 macrophages explored. downregulated CRC. Knockdown elevated amount CFSE-positive, migrating, invading cells, decreased apoptosis, levels markers. After overexpression miR-411-3p, all above metrics reversed cells. targeted negative regulation expression, further enhanced promotional effect knockdown progression polarization. Furthermore, upregulated level, Ki-67-positive count, induced vivo. upregulates induces polarization, which turn promotes

Language: Английский

Citations

0
Dysregulation of miR-411 in cancer: Causative factor for pathogenesis, diagnosis and prognosis DOI Open Access

Mengsha Zou,

Jinze Shen, Yuchen Wu

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2022, Volume and Issue: 149, P. 112896 - 112896

Published: March 29, 2022

MiRNA accounts for 1-3% of genes but regulates more than 30% gene expression in humans. This article analyzes the current deficiencies and challenges miR-411 research looks forward to prospects cancer. MiR-411 is a non-coding RNA located on chromosome 14. abnormally expressed variety cancers. The dysregulation can affect cancer cell proliferation, invasion, migration, apoptosis, colony formation, etc. be regulated by different lncRNAs circRNAs. By targeting multiple genes, participates activation MAPK signaling pathway, PI3K/AKT/mTOR p53 Ras NF-κB Wnt/β-catenin pathway. related diagnosis, prognosis, sensitivity drugs patients. In conclusion, this work outlines molecular mechanisms cellular functions aberrant its target reveal potential value drug sensitivity.

Language: Английский

Citations

12
Competing endogenous RNA network analysis of the molecular mechanisms of ischemic stroke DOI Creative Commons
Jianmin Chen, Xiaolu Li,

Ye Yang

et al.

BMC Genomics, Journal Year: 2023, Volume and Issue: 24(1)

Published: Feb. 8, 2023

Ischemic stroke (IS) is a serious neurological disease that largely results in long-term disability and death. Extensive evidence has indicated the activation of inflammation ferroptosis significantly contribute to development IS pathology. However, underlying molecular mechanism remains unclear. In this study, we aimed identify potential biomarkers associated with through construction competing endogenous RNA (ceRNA) network investigate possible inflammatory ferroptosis-related mechanisms.We identified 178 differentially expressed target messenger RNAs (DETmRNAs) IS. As revealed enrichment analysis, DEmRNAs were mainly enriched signaling pathways also related mechanism. The CIBERSORT algorithm showed immune infiltration landscapes which naïve B cells, T monocytes had statistically different numbers cerebral infarction group compared control group. A ceRNA was constructed study involving 44 long non-coding (lncRNAs), 15 microRNAs (miRNAs), 160 (mRNAs). We used receiver operating characteristic (ROC) analysis three miRNAs (miR-103a-3p, miR-140-3p, miR-17-5p), one mRNA (TLR4), lncRNA (NEAT1) as key network. shown be highly expression these further validated by method quantitative real-time polymerase chain reaction SH-SY5Y consistent findings predicted bioinformatics.Our suggest may exert an important role mechanisms IS, providing new insight into therapeutic targets.

Language: Английский

Citations

6