The mechanistic study of codonopsis pilosula on laryngeal squamous cell carcinoma based on network pharmacology and experimental validation DOI Creative Commons

Huina Guo,

Yi-Han Lou,

Xiaofang Hou

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck, with poor prognosis for advanced patients, there an urgent need to find new treatment strategies. Codonopsis pilosula, traditional Chinese medicinal herb, possesses various pharmacological activities, but its antitumor effects mechanisms in LSCC are still unclear. The aim this study was systematically investigate potential mechanism pilosula LSCC. In study, we screened effective compounds targets by TCMSP, ETCM BATMAN-TCM databases, related combining DisGeNET, GeneCards database Cytoscape software. KEGG pathway enrichment analysis utilized explore signaling pathways. core were further based on TCGA GEO analysis, molecular docking carried out predict their binding ability compounds. presence key verified LC-MS, MAPK3 expression detected qPCR tissues, knockdown proliferation, migration, invasion, cycle, apoptosis cells evaluated cellular function assays. 22 that might regulate network pharmacology. showed pilosula-LSCC mainly involved HIF-1, TNF, IL-17 FoxO Based identified as target pilosula-LSCC. results variety from had strong abilities MAPK3, among them, Caprylic Acid, Emodin Luteolin have been confirmed LC-MS. QPCR indicated highly expressed tissues. significantly inhibits migration invasion. It also suppresses growth blocking cycle inducing apoptosis. exerts through regulation multiple pathways, providing theoretical basis clinical application.

Language: Английский

The mechanistic study of codonopsis pilosula on laryngeal squamous cell carcinoma based on network pharmacology and experimental validation DOI Creative Commons

Huina Guo,

Yi-Han Lou,

Xiaofang Hou

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 25, 2025

Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor of the head and neck, with poor prognosis for advanced patients, there an urgent need to find new treatment strategies. Codonopsis pilosula, traditional Chinese medicinal herb, possesses various pharmacological activities, but its antitumor effects mechanisms in LSCC are still unclear. The aim this study was systematically investigate potential mechanism pilosula LSCC. In study, we screened effective compounds targets by TCMSP, ETCM BATMAN-TCM databases, related combining DisGeNET, GeneCards database Cytoscape software. KEGG pathway enrichment analysis utilized explore signaling pathways. core were further based on TCGA GEO analysis, molecular docking carried out predict their binding ability compounds. presence key verified LC-MS, MAPK3 expression detected qPCR tissues, knockdown proliferation, migration, invasion, cycle, apoptosis cells evaluated cellular function assays. 22 that might regulate network pharmacology. showed pilosula-LSCC mainly involved HIF-1, TNF, IL-17 FoxO Based identified as target pilosula-LSCC. results variety from had strong abilities MAPK3, among them, Caprylic Acid, Emodin Luteolin have been confirmed LC-MS. QPCR indicated highly expressed tissues. significantly inhibits migration invasion. It also suppresses growth blocking cycle inducing apoptosis. exerts through regulation multiple pathways, providing theoretical basis clinical application.

Language: Английский

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