M6A methyltransferase METTL3 promotes glucose metabolism hub gene expression and induces metabolic dysfunction-associated steatotic liver disease (MASLD)

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: Feb. 24, 2025

N6-methyladenosine (m6A) RNA modification plays a crucial role in various biological events and is implicated metabolic-related diseases. However, its MASLD remains unclear. This study aims to investigate the impact of METTL3 on through multi-omics analysis, with focus exploring potential mechanisms action. An mouse model was established by feeding C57BL/6J mice high-fat diet for 12 weeks. A stable overexpression AML12 cell also constructed via lentiviral transfection. Subsequent transcriptomic proteomic analyses, as well integrated analysis between different omics datasets, were conducted. expression significantly increased model. Through our we identified 848 genes significant inconsistencies datasets. GO/ KEGG enrichment analyses terms that may be involved post-transcriptional modifications, particularly METTL3-mediated m6A modification. Subsequently, METTL3-overexpressed model, selected top 20 co-upregulated co-downregulated main processes influenced during MASLD. By intersecting pathways obtained from previous affected METTL3-induced Protein-protein interaction proteins these highlighted GAPDH TPI1 two key hub genes. During MASLD, regulates glycolytic pathway modification, influencing occurrence development disease TPI1. These findings expand understanding provide strong evidence therapeutic targets drug development.

Language: Английский

Metabolomic profiles of body shapes and their associations with the risk of incident cardiovascular disease in Chinese adults

Nutrition Metabolism and Cardiovascular Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 104031 - 104031

Published: April 1, 2025

Language: Английский

Impact of Dietary Niacin on Metabolic Dysfunction-Associated Steatotic Liver Disease in Mediterranean Subjects: A Population-Based Study

Nutrients, Journal Year: 2024, Volume and Issue: 16(23), P. 4178 - 4178

Published: Nov. 30, 2024

The impact of dietary niacin on metabolic dysfunction-associated steatotic liver disease (MASLD) is elusive. This sub-study aimed to investigate the relationship between intake and presence MASLD in participants from two Catalonian cohorts.

Language: Английский