Papain-Based Solubilization of Decellularized Extracellular Matrix for the Preparation of Bioactive, Thermosensitive Pregels

Biomacromolecules, Journal Year: 2023, Volume and Issue: 24(12), P. 5620 - 5637

Published: Nov. 27, 2023

Solubilized, gel-forming decellularized extracellular matrix (dECM) is used in a wide range of basic and translational research due to its inherent bioactivity can promote structural functional tissue remodeling. The animal-derived protease pepsin has become the standard proteolytic enzyme for solubilization almost all types collagen-based dECM. In this study, was compared with papain, α-amylase, collagenase their potential solubilize porcine liver Maximum preservation bioactive components native dECM properties as decisive criterion further application enzymes, emphasis on minimal destruction protein structure maintained capacity physical thermogelation at neutral pH. solubilized digests, and/or physically gelled hydrogels were characterized rheological properties, gelation kinetics, GAG content, proteomic composition, growth factor profile. This study highlights papain plant-derived that serve cost-effective alternative efficient resulting homogeneous papain-digested preserved thermally triggered similar corresponding demonstrated enhanced bioadhesiveness single-cell force spectroscopy experiments fibroblasts. viability proliferation human HepaRG cells gels those pure rat tail collagen type I gels. Papain not only highly effective economically attractive but also particularly interesting when digesting human-tissue-derived regenerative applications, where materials are be avoided.

Language: Английский

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Circulating proliferative factors versus portal inflow redistribution: mechanistic insights of ALPPS-derived rapid liver regeneration

Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14

Published: Jan. 7, 2025

Associating liver partition and portal vein ligation for staged hepatectomy (ALPPS) can induce accelerated regeneration of future remnant (FLR) effectively reduce the occurrence failure due to insufficient FLR after hepatectomy, thereby increasing probability radical resection previously inoperable patients with cancer. However, exact mechanism by which ALPPS accelerates remains elusive. A review literature was performed utilizing MEDLINE/PubMed Web Science databases in March 2024. The key words "liver regeneration/hypertrophy", "portal ligation/embolization", "two-stage hepatectomy", partition/split" "future remnant" combination "mechanisms", "hemodynamics", "cytokines", "growth factors" or "collaterals" were searched title and/or abstract. references relevant articles reviewed identify additional eligible publications. Previously, a widely accepted view is that primary role splitting stage 1 accelerate promoting proliferative factor release, but evidence recent years reveal not circulating factors, hemodynamic alternations caused parenchyma transection play pivotal ALPPS-associated rapid hypertrophy. Parenchyma transection-induced are main triggers driving forces following ALPPS. release factors seems be secondary response plays an auxiliary this process.

Language: Английский

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Influencing factors and mechanism of hepatocyte regeneration

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 30, 2025

As a research hotspot in the field of regenerative medicine, hepatocyte regeneration has great potential treatment liver diseases. This paper comprehensively summarizes diverse sources and its complex influencing factors, deeply discusses typical mechanism. According to existing research, we observed that Wnt signaling pathway Notch can play synergistic role process regeneration. So further analyzed crosstalk between signal cross mechanism with TGF-β, YAP/TAZ during Despite remarkable progress study at cellular molecular levels, comprehensive understanding fine regulation factors interaction mechanisms still needs be deepened. aims systematically analyze classical by integrating multi-group data advanced bioinformatics methods, so as provide feasible ideas for diseases lay solid theoretical foundation future development medicine. It is believed focusing on rational innovative means such inducing gene tendentiousness expression anti-aging therapy, in-depth analysis interactive network are expected open up new road more effective strategies

Language: Английский

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Role of mitogens in normal and pathological liver regeneration

Hepatology Communications, Journal Year: 2025, Volume and Issue: 9(5)

Published: April 30, 2025

The liver has a unique ability to regenerate meet the body's metabolic needs, even following acute or chronic injuries. cellular and molecular mechanisms underlying normal regeneration have been well investigated improve organ transplantation outcomes. Once is impaired, pathological occurs, require further investigations. Nevertheless, plethora of cytokines growth factor-mediated pathways reported modulate physiological regeneration. Regenerative mitogens play an essential role in hepatocyte proliferation. Accelerator synergism with regenerative ones promote hepatectomy. Finally, terminator restore proliferating status hepatocytes differentiated quiescent state upon completion Chronic loss hepatocytes, which can manifest disorders any etiology, often undesired structural consequences, including fibrosis, cirrhosis, neoplasia due unregulated proliferation remaining hepatocytes. In fact, impairment function results progression current review, we intend highlight updated involved discuss impairments central regulating responsible for

Language: Английский

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Comparison of the effect of isoflurane and propofol on liver regeneration after donor hepatectomy - A randomized controlled trial

Journal of Clinical Anesthesia, Journal Year: 2025, Volume and Issue: 104, P. 111859 - 111859

Published: May 9, 2025

Language: Английский

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Chemically modified mRNA beyond COVID-19: Potential preventive and therapeutic applications for targeting chronic diseases

Biomedicine & Pharmacotherapy, Journal Year: 2021, Volume and Issue: 145, P. 112385 - 112385

Published: Oct. 28, 2021

Chemically modified mRNA represents a unique, efficient, and straightforward approach to produce class of biopharmaceutical agents. It has been already approved as vaccination-based method for targeting SARS-CoV-2 virus. The COVID-19 pandemic highlighted the prospect synthetic efficiently safely combat various diseases. Recently, optimization advances have adopted overcome limitations associated with conventional gene therapeutics leading wide-ranging applications in different disease conditions. This review sheds light on emerging directions chemically mRNAs prevent treat widespread chronic diseases, including metabolic disorders, cancer vaccination immunotherapy, musculoskeletal respiratory conditions, cardiovascular liver

Language: Английский

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Recent Advances in Liver Tissue Engineering as an Alternative and Complementary Approach for Liver Transplantation

Current Issues in Molecular Biology, Journal Year: 2023, Volume and Issue: 46(1), P. 262 - 278

Published: Dec. 29, 2023

Acute and chronic liver diseases cause significant morbidity mortality worldwide, affecting millions of people. Liver transplantation is the primary intervention method, replacing a non-functional with functional one. However, field faces challenges such as donor shortage, postoperative complications, immune rejection, ethical problems. Consequently, there an urgent need for alternative therapies that can complement traditional or serve method. In this review, we explore potential tissue engineering supplementary approach to transplantation, offering benefits patients severe dysfunctions.

Language: Английский

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Molecular Pathways Governing the Termination of Liver Regeneration

Pharmacological Reviews, Journal Year: 2024, Volume and Issue: 76(3), P. 500 - 558

Published: March 4, 2024

Abstract

The liver has the unique capacity to regenerate, and up 70% of can be removed without detrimental consequences organism. Liver regeneration is a complex process involving multiple signaling networks organs. proceeds through three phases: initiation phase, growth termination phase. Termination occurs when reaches liver-to-body weight that required for homeostasis, so-called "hepatostat." phases have been subject many studies. molecular pathways govern however, remain fully elucidated. This review summarizes molecules signal cessation regrowth after partial hepatectomy answers question, "What factors drive hepatostat?"

Significance Statement

Unraveling underlying enables identification druggable targets will allow us gain pharmacological control over regeneration. For these purposes, it would useful understand why regenerative hampered under certain pathological circumstances so as artificially modulate processes (e.g., by blocking pathways) improve clinical outcomes safeguard patient's life.

Language: Английский

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Biosensors: A novel approach to and recent discovery in detection of cytokines

Cytokine, Journal Year: 2020, Volume and Issue: 136, P. 155272 - 155272

Published: Sept. 9, 2020

Language: Английский

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Metabolism of N-nitrosodimethylamine, methylation of macromolecules, and development of hepatic fibrosis in rodent models

Journal of Molecular Medicine, Journal Year: 2020, Volume and Issue: 98(9), P. 1203 - 1213

Published: July 14, 2020

Language: Английский

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