Histological evaluation of the regenerative potential of a novel photocrosslinkable gelatin-treated dentin matrix hydrogel in direct pulp capping: an animal study

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Jan. 19, 2024

Abstract Background To assess histologically the success of pulp capping approach performed in traumatically exposed dogs’ teeth using a novel injectable gelatin-treated dentin matrix light cured hydrogel (LCG-TDM) compared with LCG, MTA and TheraCal LC. Methods Sixty-four were divided into two groups (each including 32 teeth) based on post-treatment evaluation period: group I: 2 weeks II: 8 weeks. Each was further subdivided according to material four subgroups ( n = 8), subgroup A (light-cured gelatin hydrogel) as control subgroup, B (LCG-TDM), C (TheraCal LC), D (MTA). Pulps mechanically middle cavity floor capped different materials. An assessment periapical response preoperatively at After 8-week intervals, dogs sacrificed, stained hematoxylin-eosin graded by histologic scoring system. Statistical analysis chi-square Kruskal-Wallis tests p 0.05). Results All showed mild inflammation normal tissue no significant differences between ≤ 0.05), except for LC which exhibited moderate (62.5%). Absence complete calcified bridge reported all weeks, while majority samples LCG-TDM MTA-Angelus formation absence inflammatory them However, formed significantly thicker, layers ordered odontoblasts identified create homogeneous tubular structure numerous dentinal tubule lines suggesting favourable trend towards regeneration. revealed reasonably thick (50%) LCG heavily fibrous infiltrates areas degenerated signs hard formation. Conclusions LCG-TDM, an extracellular matrix-based material, has potential regenerate preserve vitality, making it viable natural alternative silicate-based cements healing vivo defects direct pulp-capping procedures.

Language: Английский

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Hard tissue formation in pulpotomized primary teeth in dogs with nanomaterials MCM-48 and MCM-48/hydroxyapatite: an in vivo animal study

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: March 11, 2024

Abstract Background This animal study sought to evaluate two novel nanomaterials for pulpotomy of primary teeth and assess the short-term pulpal response hard tissue formation in dogs. The results were compared with mineral trioxide aggregate (MTA). Methods vivo on dogs evaluated 48 premolar 4 mongrel female age 6–8 weeks, randomly divided into four groups ( n = 12). underwent complete under general anesthesia. pulp was capped MCM-48, MCM-48/Hydroxyapatite (HA), MTA (positive control), gutta-percha (negative restored intermediate restorative material (IRM) paste amalgam. After 4–6 extracted histologically analyzed agent. Results data using Kruskal‒Wallis, Fisher’s exact, Spearman’s, Mann‒Whitney tests. not significantly different regarding severity inflammation P 0.53), extent 0.72), necrosis 0.361), edema 0.52), 0.06), or connective 0.064). A significant correlation noted between r 0.954, < 0.001). frequency bone 0.012), 0.047), congestion 0.02), 0.01). No group. type newly formed among three experimental 0.320). Conclusion MCM-48 MCM-48/HA are bioactive that may serve as alternatives due their ability induce formation. mesoporous silica have potential osteogenesis tertiary (reparative) dentin

Language: Английский

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Preparation and characterization of bovine dental pulp-derived extracellular matrix hydrogel for regenerative endodontic applications: an in vitro study

BMC Oral Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 24, 2024

Language: Английский

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Temperature‐sensitive sodium beta‐glycerophosphate/chitosan hydrogel loaded with all‐trans retinoic acid regulates Pin1 to inhibit the formation of spinal cord injury‐induced rat glial scar

Bioengineering & Translational Medicine, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract Glial scar formation is a major obstacle to nerve regeneration following spinal cord injury (SCI). Pin1 and the PI3K/AKT/CDK2 signaling pathway play crucial roles in neuronal regulation, but research on their involvement glial scarring remains limited. In this study, we have for first time observed that Pin1, PI3K, AKT, CDK2 are upregulated interact with each other SCI. Further experiments revealed contributes development of scars by promoting astrocyte proliferation, inhibiting apoptosis, activating pathway. Additionally, all‐trans retinoic acid (ATRA), specific chemical inhibitor effectively suppresses expression. However, its clinical application limited short half‐life susceptibility inactivation. To address these issues, developed thermosensitive sodium beta‐glycerophosphate (β‐GP)/chitosan (CS) hydrogel loaded ATRA (β‐GP/CS@ATRA). This exhibits favorable morphology biocompatibility. Compared free ATRA, β‐GP/CS@ATRA significantly enhances functional motor recovery after SCI protects tissue, thereby formation. Mechanistically, administration blocks activation study suggests combining target expression may be promising strategy treating

Language: Английский

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