Baicalin and probenecid protect against Glaesserella parasuis challenge in a piglet model DOI Creative Commons

Shulin Fu,

Siyu Liu,

Jingyang Li

et al.

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: July 29, 2024

Abstract Glaesserella parasuis ( G. ) induces vascular damage and systemic inflammation. However, the mechanism by which it causes is currently unclear. Baicalin has important anti-inflammatory, antibacterial immunomodulatory functions. In this study, we explored ability of baicalin probenecid to protect against challenge in a piglet model. Sixty piglets were randomly divided into control group; an infection 25 mg/kg, 50 mg/kg 100 groups. The group groups injected intramuscularly with 20 body weight (BW) BW, BW baicalin, respectively. All except those from intraperitoneally 1 × 10 8 CFU parasuis. was TSB. results showed protected challenge, improved decreased temperature changes piglets. attenuated IL-1β, IL-10, IL-18, TNF-α IFN-γ mRNA levels blood for 48 h, inhibited production nucleosides ATP, ADP, AMP UMP 24 72 reduced Panx-1/P2Y6/P2X7 expression, weakened NF-kB, AP-1, NLRP3/Caspase-1 ROCK/MLCK/MLC signalling activation, upregulated VE-cadherin expression vessels challenged . alleviated pathological tissue induced Our might provide promising strategy treat clinical setting.

Language: Английский

Synaptic plasticity and neuroprotection: The molecular impact of flavonoids on neurodegenerative disease progression DOI

Spandana Rajendra Kopalli,

Tapan Behl,

Ashishkumar Kyada

et al.

Neuroscience, Journal Year: 2025, Volume and Issue: 569, P. 161 - 183

Published: Feb. 7, 2025

Language: Английский

Citations

1
USP48 inhibits colorectal cancer progression and promotes M1-like macrophage polarization by stabilizing TAK1 DOI
Xinwen Zhang, Jiawei Zhao,

Guangying Qi

et al.

Experimental Cell Research, Journal Year: 2025, Volume and Issue: 446(1), P. 114469 - 114469

Published: Feb. 18, 2025

Language: Английский

Citations

0
Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/Akt/mTOR and RAS/MEK/ERK signalling pathways DOI Creative Commons

Shulin Fu,

Jingyang Li,

Jiarui You

et al.

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: July 29, 2024

Abstract Infection of piglets with Glaesserella parasuis ( G. ) induces host immunosuppression. However, the mechanism underlying immunosuppression remains unclear. Activation PD-1/PD-L1 axis has been shown to trigger Baicalin possesses anti-inflammatory and immunomodulatory functions. whether baicalin inhibits activation thus alleviates not investigated. In this study, effect on attenuation piglet induced by was evaluated. Seventy were randomly divided into control group, infection levamisole BMS-1 25 mg/kg 50 group 100 group. Following pretreatment levamisole, or baicalin, challenged 1 × 10 8 CFU . Our results showed that modified routine blood indicators biochemical parameters; downregulated IL-1β, IL-10, IL-18, TNF-α IFN-γ mRNA expression; upregulated IL-2 IL-8 expression in blood. Baicalin, increased proportions CD3 + T cells, CD4 CD8 cells – CD21 B splenocyte population, blood, inhibited TIM-3 activation. reduced p-PI3K, p-Akt, p-mTOR expression, p-MEK1/2/MEK1/2 p-ERK1/2/ERK1/2 ratios RAS expression. provided substantial protection against challenge relieved tissue histopathological damage. findings might provide new strategies for controlling other immunosuppressive diseases.

Language: Английский

Citations

1
Baicalin-loaded proline and hydroxy proline functionalized chitosan derivative nanofiber composite as burning wound dressings DOI

Azam Sharifi,

Sakineh Mashjoor,

Behzad Sharif Makhmalzadeh

et al.

Applied Materials Today, Journal Year: 2024, Volume and Issue: 41, P. 102519 - 102519

Published: Nov. 16, 2024

Language: Английский

Citations

1
Baicalin and probenecid protect against Glaesserella parasuis challenge in a piglet model DOI Creative Commons

Shulin Fu,

Siyu Liu,

Jingyang Li

et al.

Veterinary Research, Journal Year: 2024, Volume and Issue: 55(1)

Published: July 29, 2024

Abstract Glaesserella parasuis ( G. ) induces vascular damage and systemic inflammation. However, the mechanism by which it causes is currently unclear. Baicalin has important anti-inflammatory, antibacterial immunomodulatory functions. In this study, we explored ability of baicalin probenecid to protect against challenge in a piglet model. Sixty piglets were randomly divided into control group; an infection 25 mg/kg, 50 mg/kg 100 groups. The group groups injected intramuscularly with 20 body weight (BW) BW, BW baicalin, respectively. All except those from intraperitoneally 1 × 10 8 CFU parasuis. was TSB. results showed protected challenge, improved decreased temperature changes piglets. attenuated IL-1β, IL-10, IL-18, TNF-α IFN-γ mRNA levels blood for 48 h, inhibited production nucleosides ATP, ADP, AMP UMP 24 72 reduced Panx-1/P2Y6/P2X7 expression, weakened NF-kB, AP-1, NLRP3/Caspase-1 ROCK/MLCK/MLC signalling activation, upregulated VE-cadherin expression vessels challenged . alleviated pathological tissue induced Our might provide promising strategy treat clinical setting.

Language: Английский

Citations

0