Phytochemical analysis, in-vitro and in-silico study of antiproliferative activity of ethyl acetate fraction of Launaea cornuta (Hochst. ex Oliv. & Hiern) C. Jeffrey against human cervical cancer cell line

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: June 28, 2024

Introduction: Cervical cancer is one of the leading causes death among women globally due to limitation current treatment methods and their associated adverse side effects. Launaea cornuta used as traditional medicine for a variety diseases including cancer. However, there no scientific validation on antiproliferative activity L. against cervical Objective: This study aimed evaluate selective antiproliferative, cytotoxic antimigratory effects explore its therapeutical mechanisms in human cell lines (HeLa-229) through network analysis approach. Materials methods: The effect ethyl acetate fraction proliferation cells was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) bioassay assessed wound healing assays. Compounds were analysed using qualitative colour method gas chromatography-mass spectroscopy (GC-MS). Subsequently, bioinformatic analyses, protein-protein interaction (PPI) analysis, Gene Ontology (GO), Kyoto Encyclopaedia Genes Genomes (KEGG) performed screen potential anticervical therapeutic target genes cornuta. Molecular docking (MD) predict understand molecular interactions ligands Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) validate results. Results: exhibited remarkable HeLa-229 (IC 50 20.56 ± 2.83 μg/mL) with selectivity index (SI) 2.36 minimal cytotoxicity non-cancerous (Vero-CCL 81 48.83 23.02). preliminary screening revealed presence glycosides, phenols, saponins, terpenoids, quinones, tannins. Thirteen compounds also identified GC-MS analysis. 124 obtained, AKT1, MDM2, CDK2, MCL1 MTOR top hub PI3K/Akt1, Ras/MAPK, FoxO EGFR signalling pathways significantly enriched pathways. results showed that stigmasteryl methyl ether had good binding affinity ATK1, BCL2, Casp9, energy ranging from −7.0 −12.6 kcal/mol. Tremulone TP53 P21 −8.0 kcal/mol, respectively. suggests stable selected Furthermore, RT-qPCR MDM2 BCL2 downregulated, Casp9 upregulated treated compared negative control (DMSO 0.2%). Conclusion: findings indicate phytochemicals modulates various targets exhibit cells. lays foundation further research develop innovative clinical agents.

Language: Английский

Syringin as a novel therapeutic agent for renal cell carcinoma by targeting EGFR/PI3K/Akt pathway and enhancing sunitinib efficacy

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 123, P. 106556 - 106556

Published: Nov. 13, 2024

Language: Английский