Texasin, A main product from Caragana Jubata (Pall.) Poir, induces proliferation arrest and protective autophagy in lung adenocarcinoma DOI Creative Commons

Liuzhao Cao,

Tiantian Li,

Xingxiang Xu

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 20, 2025

Lung cancer, a leading cause of mortality worldwide, necessitates effective therapeutic strategies. Caragana jubata, traditional Chinese medicinal plant, harbors Texasin, potential anti-tumor agent. This study aimed to evaluate the anti-cancer effects Texasin on lung cancer cells, while assessing its impact normal cells. The utilized cell lines H1299 and A549, alongside embryonic investigate Texasin's through Cell Counting Kit-8, Transwell, wound healing assays. Transcriptome sequencing analysis were performed identify mechanisms. β-galactosidase activity Retinoblastoma(RB) protein expression assessed, autophagy apoptosis explored chloroquine co-treatment. Mice bearing cell-derived tumors treated with Texasin. Tumor changes assessed in vivo imaging, levels within analyzed. inhibited proliferation, migration, invasion without harming It promoted senescence, arrested cycle G1 phase, upregulated RB expression. induced protective autophagy, which could be converted by inhibits proliferation adenocarcinoma cells vivo, as evidenced immunohistochemistry showing an increase tumors. emerges promising non-cytotoxic anti-lung compound, significantly inhibiting malignant phenotypes, highlighting for therapy.

Language: Английский

Texasin, A main product from Caragana Jubata (Pall.) Poir, induces proliferation arrest and protective autophagy in lung adenocarcinoma DOI Creative Commons

Liuzhao Cao,

Tiantian Li,

Xingxiang Xu

et al.

BMC Cancer, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 20, 2025

Lung cancer, a leading cause of mortality worldwide, necessitates effective therapeutic strategies. Caragana jubata, traditional Chinese medicinal plant, harbors Texasin, potential anti-tumor agent. This study aimed to evaluate the anti-cancer effects Texasin on lung cancer cells, while assessing its impact normal cells. The utilized cell lines H1299 and A549, alongside embryonic investigate Texasin's through Cell Counting Kit-8, Transwell, wound healing assays. Transcriptome sequencing analysis were performed identify mechanisms. β-galactosidase activity Retinoblastoma(RB) protein expression assessed, autophagy apoptosis explored chloroquine co-treatment. Mice bearing cell-derived tumors treated with Texasin. Tumor changes assessed in vivo imaging, levels within analyzed. inhibited proliferation, migration, invasion without harming It promoted senescence, arrested cycle G1 phase, upregulated RB expression. induced protective autophagy, which could be converted by inhibits proliferation adenocarcinoma cells vivo, as evidenced immunohistochemistry showing an increase tumors. emerges promising non-cytotoxic anti-lung compound, significantly inhibiting malignant phenotypes, highlighting for therapy.

Language: Английский

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