International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 26(1), P. 76 - 76
Published: Dec. 25, 2024
Multidrug-resistant bacteria represent a significant challenge in the treatment of bacterial infections, often leading to therapeutic failures. This issue underlines need develop strategies that improve efficacy conventional antibiotic therapies. In this study, we aimed assess whether plant-derived compound, α-mangostin, and photodynamic therapy (PDT) could enhance antibacterial activity ciprofloxacin against uropathogenic strains Escherichia coli Staphylococcus aureus. Using nanopore sequencing technology, confirmed clinical tested were classified as multidrug-resistant. Digital holotomography (DHT) was used examine α-mangostin-induced changes cells’ penetration by photosensitizer. A scanning confocal fluorescence microscope visualize photosensitizer into cells validate DHT results. synergistic effect between α-mangostin observed exclusively S. aureus strains, while no enhancement ciprofloxacin’s detected E. when combined with α-mangostin. Notably, significantly potentiated effects its combination compared untreated controls. addition, morphological exposed these antimicrobials. conclusion, our findings suggest PDT may serve valuable adjuncts ciprofloxacin, improving eradication uropathogens.
Language: Английский