Single Cell Sequencing Provides Clues about the Developmental Genetic Basis of Evolutionary Adaptations in Syngnathid Fishes DOI Open Access
Hope M. Healey, Hayden B. Penn, Clayton M. Small

et al.

Published: Nov. 27, 2024

Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, dermal bony armor. The developmental genetic cellular changes led to evolution these largely unknown. Recent syngnathid genome assemblies revealed suggestive gene content differences provide opportunity for detailed analyses. We created a single cell RNA sequencing atlas Gulf pipefish embryos understand basis four traits: derived head shape, toothlessness, armor, pregnancy. completed marker analyses, built networks, examined spatial expression select genes. identified osteochondrogenic mesenchymal cells in elongating face express regulatory genes bmp4, sfrp1a , prdm16 . found no evidence tooth primordia cells, we observed re-deployment osteoblast networks developing Finally, epidermal expressed nutrient processing environmental sensing genes, potentially relevant brooding environment. evolutionary innovations composed recognizable types, suggesting features originate within existing networks. Future work addressing across multiple stages species is essential understanding how their novelties evolved.

Language: Английский

Independent Evolution of Sex Chromosomes and Male Pregnancy–Related Genes in Two Seahorse Species DOI Creative Commons
Xin Long, Deborah Charlesworth, Jianfei Qi

et al.

Molecular Biology and Evolution, Journal Year: 2022, Volume and Issue: 40(1)

Published: Dec. 29, 2022

Unlike birds and mammals, many teleosts have homomorphic sex chromosomes, changes in the chromosome carrying sex-determining locus, termed "turnovers", are common. Recent turnovers allow studies of several interesting questions. One question is whether new regions evolve to become completely non-recombining, if so, how why. Another (as predicted) evolutionary that benefit one accumulate newly sex-linked region. To study these questions, we analyzed genome sequences two seahorse species Syngnathidae, a fish group which evolved unique structure, male brood pouch. We find both XY systems, but their pairs not homologs, implying at least turnover event has occurred. The Y-linked occupy 63.9% 95.1% entire do exhibit extensive sequence divergence with X-linked homologs. evidence for occasional recombination between extant chromosomes may account homomorphism. argue did by suppression after turnover, ancestral nature low crossover rates regions. With such an landscape, can instantly create Finally, test adaptive evolution pouch-related genes they became seahorse.

Language: Английский

Citations

12
Cross-sexual Transfer Revisited DOI Creative Commons
Andrew P. Anderson, Jay J. Falk

Integrative and Comparative Biology, Journal Year: 2023, Volume and Issue: 63(4), P. 936 - 945

Published: May 5, 2023

In her influential book "Developmental Plasticity and Evolution," Mary Jane West-Eberhard introduced the concept of cross-sexual transfer, where traits expressed in one sex an ancestral species become other sex. Despite its potential ubiquity, we find that transfer has been under-studied under-cited literature, with only a few experimental papers have invoked concept. Here, aim to reintroduce as powerful framework for explaining variation highlight relevance current studies on evolution sexual heteromorphism (different means or modes trait values between sexes). We discuss several exemplary published past two decades, further building West-Eberhard's extensive review. emphasize scenarios avenues study, within-sex polymorphic sex-role reversed species, evolutionary adaptive implications. Lastly, propose future questions expand our understanding from nonhormonal mechanisms identification broad taxonomic patterns. As biologists increasingly recognize nonbinary often continuous nature heteromorphism, important utility generating novel insights perspectives phenotypes across diverse taxa.

Language: Английский

Citations

4
Range-wide population genomics of common seadragons shows secondary contact over a former barrier and insights on illegal capture DOI Creative Commons
Josefin Stiller, Nerida G. Wilson, Greg W. Rouse

et al.

BMC Biology, Journal Year: 2023, Volume and Issue: 21(1)

Published: May 29, 2023

Abstract Background Common seadragons ( Phyllopteryx taeniolatus , Syngnathidae) are an emblem of the diverse endemic fauna Australia’s southern rocky reefs, newly recognized “Great Southern Reef.” A lack assessments spanning this global biodiversity hotspot in its entirety is currently hampering understanding factors that have contributed to diversity. The common seadragon has a wide range across Australia's entire temperate south and includes geogenetic break over former land bridge, which called status as single species into question. As popular aquarium display sells for high prices, also vulnerable illegal capture. Results Here, we provide range-wide nuclear sequences (986 variable Ultraconserved Elements) 198 individuals mitochondrial genomes 140 assess status, identify genetic units their diversity, trace source two poached individuals. Using published data other species, found lineages diverged relatively recently (< 0.63 Ma). Within seadragons, pronounced structure, falling three major groups western, central, eastern parts range. While populations Bassian Isthmus were divergent, there evidence secondary contact since passage opened. We strong cline diversity from center tapering symmetrically towards peripheries. Based on similarities, inferred originated around Albany southwestern Australia. Conclusions conclude constitute with geographic structure but coherence through gene flow. low east west coasts concerning given these areas projected face fast climate change. Our results suggest addition life history, geological events demographic expansions all played role shaping south. These insights important step historical determinants Great Reef.

Language: Английский

Citations

4
Single Cell RNA Sequencing Provides Clues for the Developmental Genetic Basis of Syngnathidae’s Evolutionary Adaptations DOI Open Access
Hope M. Healey, Hayden B. Penn, Clayton M. Small

et al.

Published: June 5, 2024

Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, dermal bony armor. The developmental genetic cellular changes led to evolution these largely unknown. Recent syngnathid genomes revealed suggestive gene content differences provide opportunity for detailed analyses. We created a single cell RNA sequencing atlas Gulf pipefish embryos understand basis four traits: derived head shape, toothlessness, armor, pregnancy. completed marker analyses, built networks, examined spatial expression select genes. identified osteochondrogenic mesenchymal cells in elongating face express regulatory genes bmp4, sfrp1a , prdm16 . found no evidence tooth primordia cells, we observed re-deployment osteoblast networks developing armor.Finally, epidermal expressed nutrient processing environmental sensing genes, potentially relevant brooding environment. evolutionary innovations composed recognizable types, suggesting features originate within existing networks. Future work addressing across multiple stages species is essential understanding how their novelties evolved.

Language: Английский

Citations

1
Phylogeography of Microphis retzii (Bleeker, 1856) and Microphis brachyurus (Bleeker, 1854) in the Pacific DOI Creative Commons
Vincent Haÿ, Marion I. Mennesson, Camille Carpentier

et al.

Journal of Fish Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Abstract Syngnathidae are a charismatic family of teleost fishes, represented by seahorses, seadragons, and pipefishes. mainly composed marine species, but about 30 species pipefishes inhabit freshwater insular environments the Indo‐Pacific realm. Recent research has shown that some pipefish amphidromous exhibit high intraspecific divergences across their distribution range, like Microphis brachyurus (Bleeker, 1854) distributed from Sri Lanka to French Polynesia retzii 1856) Taiwan Indonesia. In this study, we used mitochondrial cytochrome oxidase I ( COI ) partial gene 91 specimens M. localities representative respective area improve knowledge on population structure these two widespread species. Genetic delimitation phylogeographic analyses were combined explore spatial patterns genetic diversity ranges We have highlighted deep structuring within relate results various biotic abiotic factors. For , in is distinct those West Pacific, suggesting its distinctiveness recognition as an evolutionary significant unit (ESU). three lineages delimited range distribution, existence Southeast Asia (Bali/Java/Lombok China/Taiwan). Pipefish particularly vulnerable anthropogenic pressures (inherent environments). The present results, revising geographic will help implement effective conservation management measures.

Language: Английский

Citations

1
Colonization Dynamics Explain the Decoupling of Species Richness and Morphological Disparity in Syngnatharian Fishes Across Oceans DOI
Aintzane Santaquiteria, Elizabeth Christina Miller, Ulises Rosas‐Puchuri

et al.

The American Naturalist, Journal Year: 2024, Volume and Issue: 205(3), P. E80 - E99

Published: Nov. 12, 2024

AbstractA clear longitudinal gradient in species richness across oceans is observed extant marine fishes, with the Indo-Pacific exhibiting greatest diversity. Three non-mutually-exclusive evolutionary hypotheses have been proposed to explain this diversity gradient: time for speciation, center of accumulation, and situ diversification rates. Using morphologically disparate syngnatharians (seahorses, dragonets, goatfishes, relatives) as a study system, we tested these additionally assessed whether patterns morphological are congruent patterns. We used well-sampled phylogenies suite phylogenetic comparative methods (including novel phylogenetically corrected Kruskal-Wallis test) that account various sources uncertainty estimate rates lineage disparity within all three major oceanic realms (Indo-Pacific, Atlantic, eastern Pacific), well region. find similar regions, indicating increased syngnatharian due earlier colonizations from Tethys Sea followed by speciation more frequent during Miocene coinciding formation coral reefs. These results support both accumulation hypotheses. Unlike unevenness, shape because early origin body plans their subsequent spread via colonization rather than evolution. Our illustrate how became decoupled biodiversity hot spot.

Language: Английский

Citations

1
Syngnathoid Evolutionary History and the Conundrum of Fossil Misplacement DOI Creative Commons
Chase Doran Brownstein

Integrative Organismal Biology, Journal Year: 2023, Volume and Issue: 5(1)

Published: Jan. 1, 2023

Synopsis Seahorses, pipefishes, trumpetfishes, shrimpfishes, and allies are a speciose, globally distributed clade of fishes that have evolved large number unusual body plans. The includes all these forms, Syngnathoidei, has become model for the study life history evolution, population biology, biogeography. Yet, timeline syngnathoid evolution remained highly contentious. This debate is largely attributable to nature fossil record, which both poorly described patchy several major lineages. Although syngnathoids been used calibrate molecular phylogenies, interrelationships extinct species their affinities living clades scarcely quantitatively tested. Here, I use an expanded morphological dataset reconstruct evolutionary relationships ages extant syngnathoids. Phylogenies generated using different analytical methodologies congruent with phylogenetic trees Syngnathoidei but consistently find novel placements key taxa as calibrators in phylogenomic studies. Tip-dating phylogeny finds differs slightly from one inferred generally post-Cretaceous diversification event. These results emphasize importance testing species, particularly when they critical assessing divergence times.

Language: Английский

Citations

3
Why several when one can unite them all? Integrative taxonomic revision of Indo-Pacific freshwater pipefish (Nerophinae) DOI
Vincent Haÿ, Marion I. Mennesson, Clara Lord

et al.

Zoological Journal of the Linnean Society, Journal Year: 2023, Volume and Issue: 198(4), P. 923 - 956

Published: June 5, 2023

Abstract The family Syngnathidae (seahorses, sea dragons and pipefishes) is a fascinating group. Species are mostly distributed in marine coastal waters, but about 30 species inhabit Indo-Pacific tropical island rivers. There currently six freshwater genera of Nerophinae (trunk-brooders): Belonichthys, Coelonotus, Doryichthys, Lophocampus, Microphis Oostethus. Unlike other Syngnathidae, pipefish have been little studied; the taxonomy unclear only based on morphomeristic data. purpose our study to undertake revision this group an integrative approach. By combining molecular data from four mitochondrial markers (5415 bp), with character mapping ecological data, we now recognize single genus as valid, Microphis, opposed previously accepted. Our results also reveal one new candidate species, nicoleae sp. nov., resurrection two others: torrentius vaillantii. This shows relevance for higher taxa delineation, using integration by congruence.

Language: Английский

Citations

3
“Divergence and gene flow history at two large chromosomal inversions involved in long-snouted seahorse ecotype formation” DOI Creative Commons
Laura Meyer, Pierre Barry, Florentine Riquet

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 4, 2023

Abstract Chromosomal inversions can play an important role in divergence and reproductive isolation by building maintaining distinct allelic combinations between evolutionary lineages. Alternatively, they take the form of balanced polymorphisms that segregate within populations over time until one arrangement becomes fixed. Many questions remain about how these different inversion arise, mechanisms responsible for their long-term maintenance interact, ultimately contribute to speciation. The long-snouted seahorse ( Hippocampus guttulatus ) is known be subdivided into partially isolated lineages marine-lagoon ecotypes differentiated structural variation. Here, we aim characterise differences along entire genome, reconstruct history ecotype formation. We generated a near chromosome-level reference genome assembly described genome-wide patterns diversity through analysis 112 whole-genome sequences from Atlantic, Mediterranean, Black Sea populations. Combined with linked-read sequencing data, found evidence two megabase-scale chromosomal showing contrasted allele frequency across species range. reveal represent ancient intraspecific polymorphisms, being likely maintained divergent selection, other associative overdominance. Haplotype characterising Mediterranean also suggest existence potential interactions inversions, possibly driven environment-dependent fitness effects. Lastly, detected gene flux eroding inverted alleles at varying levels impact on dynamics.

Language: Английский

Citations

3
DNA barcodes for the pipefish genus Corythoichthys (Actinopterygii: Syngnathiformes) from the Indian Ocean provide insights into cryptic diversity DOI
Kannan Shalu, Liju Thomas, Ghosh Ramvilas

et al.

Journal of Fish Biology, Journal Year: 2023, Volume and Issue: 102(3), P. 680 - 688

Published: Jan. 5, 2023

The syngnathiform genus Corythoichthys comprises a group of taxonomically complex, tail-brooding (Syngnathinae) pipefishes widely distributed in the Indo-Pacific region. Due to presence overlapping interspecific morphological characters, reliable taxonomic information on is still lacking. Using 52 CO1 sequences, including seven newly generated, phylogenetic analysis was carried out understand genetic diversity, distribution and 'species groups' within Corythoichthys. Species delimitation using Automatic Barcode Gap Discovery (ABGD) confirmed 13 species which include 'species-complexes' previously considered as single taxon. Our results revealed three groups, 'C. amplexus', conspicillatus' haematopterus' four unidentified/undescribed wider realm. Interestingly, 60 sequences mitogenome identified GenBank are misidentified at level. Based our findings, we suggest that taxonomy systematics need be re-examined validated integrative methods, care should taken while selecting specimens for studies.

Language: Английский

Citations

2