Dynamics, diversity, and roles of bacterial transmission modes during the first asexual life stages of the freshwater sponge Spongilla lacustris DOI Creative Commons
Benoît Paix,

Elodie van der Valk,

Nicole J. de Voogd

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 23, 2024

Abstract Background: Sponges-associated bacteria play important roles in the physiology of their host, whose recruitment processes are crucial to maintain symbiotic associations. However, acquisition bacterial communities within freshwater sponges is still unexplored. Spongilla lacustris a model sponge widely distributed European rivers and lakes, producing dormant cysts (named gemmules) for asexual reproduction, before winter. Through an in vitroexperiment, this study aims describe dynamics transmission modes following hatching these gemmules. Results: An overall change β-diversity was observed through ontology new juvenile sponges. These temporal differences were potentially linked first osculum development canal system, then, increasing colonization Chlorella-like photosymbionts. Gemmules with sterilized surface found have more dispersed less diverse microbiome, revealing importance gemmule epibacteria whole holobiont stability. suggested be vertically transmitted from maternal tissues surface. Vertical incorporation inside gemmule, also as dominant mode, especially nitrogen fixers Terasakiellaceae. Finally, we showed that almost no ASVs shared between free-living community juveniles, suggesting horizontal unlikely happen during stages development. filtered probably used source nutrients, allowing enrichment copiotrophic already present its microbiome. Conclusions: This brings insight better understanding microbiome We epibacterial on gemmules stability, demonstrated near absence stages.

Language: Английский

On the origin of the functional versatility of macrophages DOI Creative Commons
Adam Bajgar, Gabriela Krejčová

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Feb. 23, 2023

Macrophages represent the most functionally versatile cells in animal body. In addition to recognizing and destroying pathogens, macrophages remove senescent exhausted cells, promote wound healing, govern tissue metabolic homeostasis. addition, many specialized populations of tissue-resident exhibit highly functions essential for function specific organs. Sometimes, however, cease perform their protective seemingly incomprehensible response certain stimuli leads pathology. this study, we address question origin functional versatility macrophages. To end, have searched evolutionary themselves emergence characteristic properties. We hypothesize that features proinflammatory evolved unicellular ancestors animals, repertoire macrophage-like amoebocytes further expanded with evolution multicellularity increasing complexity tissues organ systems. suggest entire macrophage by repurposing diversification basic early metazoans under conditions barely comparable multicellular organisms. believe applying perspective, may find an explanation otherwise counterintuitive behavior human pathologies.

Language: Английский

Citations

17
High microbiome and metabolome diversification in coexisting sponges with different bio-ecological traits DOI Creative Commons
Valerio Mazzella, Antonio Dell’Anno, Néstor Etxebarría

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: April 8, 2024

Abstract Marine Porifera host diverse microbial communities, which influence metabolism and fitness. However, functional relationships between sponge microbiomes metabolic signatures are poorly understood. We integrate microbiome characterization, metabolomics predicted functions of four coexisting Mediterranean sponges – Petrosia ficiformis , Chondrosia reniformis Crambe crambe Chondrilla nucula . Microscopy observations reveal anatomical differences in densities. Microbiomes exhibit strong species-specific trends. C. shares many rare amplicon sequence variants (ASV) with the surrounding seawater. This suggests important inputs diversity acquired by selective horizontal acquisition. Phylum Cyanobacteria is mainly represented According to putative functions, P. functionally heterotrophic, while autotrophic. The species display distinct profiles at single compound level. molecular class level they share a “core metabolome”. Concurrently, we find global microbiome-metabolome association when considering all species. Within each still, sets microbe/metabolites identified driving multi-omics congruence. Our findings suggest that players may promote niche diversification, but also, analogous phenotypic patterns “symbiont evolutionary convergence” assemblages where holobionts co-exist same area.

Language: Английский

Citations

7
Symbiosis: the other cells in development DOI Creative Commons
Tyler J. Carrier, Thomas C. G. Bosch

Development, Journal Year: 2022, Volume and Issue: 149(13)

Published: July 1, 2022

ABSTRACT Animal development is an inherently complex process that regulated by highly conserved genomic networks, and the resulting phenotype may remain plastic in response to environmental signals. Despite having been studied a more natural setting for past few decades, this framework often precludes role of microbial prokaryotes these processes. Here, we address how symbioses impact animal from onset gametogenesis through adulthood. We then provide first assessment which developmental processes or not be influenced and, doing so, holistic view budding discipline symbiosis.

Language: Английский

Citations

27
Multiomics integration for the function of bacterial outer membrane vesicles in the larval settlement of marine sponges DOI Creative Commons
Beibei Zhang,

Chenzheng Jia,

Mingyu Li

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 26, 2024

Bacterial outer membrane vesicles (OMVs) contain a variety of chemical compounds and play significant roles in maintaining symbiotic relationships changing ocean, but little is known about their function, particularly sponge larval development. During the growth

Language: Английский

Citations

5
Global patterns in symbiont selection and transmission strategies in sponges DOI Creative Commons
Cristina Díez‐Vives, Vasiliki Koutsouveli, María Conejero

et al.

Frontiers in Ecology and Evolution, Journal Year: 2022, Volume and Issue: 10

Published: Oct. 26, 2022

Sponges host dense and diverse communities of microbes (known as the microbiome) beneficial for nutrition defense. Symbionts in turn receive shelter metabolites from sponge host, making their relationship both partners. Given that sponge-microbes associations are fundamental survival both, especially sponge, such is maintained through life even passed on to future generations. In many organisms, microbiome has profound effects development but influence reproductive developmental pathways sponges less understood. sponges, oocytes, sperm, embryos, larvae vertical transmission), using a variety methods include direct uptake mesohyl phagocytosis by oocytes indirect transmission oocyte nurse cells. Such can remain elements untouched, transfer offspring, or be digested make yolky nutrient reserves larvae. When how those decisions made fundamentally unanswered questions reproduction. Here we review diversity modes existent entire phylum Porifera detailed imaging electron microscopy, available metabarcoding data elements, macroevolutionary patterns associated phylogenetic constraints. Additionally, examine fidelity this possible reasons observed variability some stages. Our current understanding marine however, adult microbial community established combination horizontal (acquisition surrounding environment each new generation) processes, although extent which mode shapes still remains determined. We also assessed role filtration, cellular structures acquiring external microbes, immune system, ultimately stable prokaryotes sponges.

Language: Английский

Citations

22
Marine heatwave conditions drive carryover effects in a temperate sponge microbiome and developmental performance DOI Creative Commons
Francesca Strano, Valerio Micaroni, Torsten Thomas

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2023, Volume and Issue: 290(2000)

Published: June 7, 2023

Marine heatwaves are increasingly subjecting organisms to unprecedented stressful conditions, but the biological consequences of these events still poorly understood. Here we experimentally tested presence carryover effects heatwave conditions on larval microbiome, settlers growth rate and metamorphosis duration temperate sponge

Language: Английский

Citations

11
From Sea to Freshwater: Shared and Unique Microbial Traits in Sponge Associated Prokaryotic Communities DOI
Michelle Guzmán de Fernandes, Gabriel Nascimento-Silva, Enrique E. Rozas

et al.

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(4)

Published: March 8, 2025

Language: Английский

Citations

0
Synthase-selected sorting approach identifies a beta-lactone synthase in a nudibranch symbiotic bacterium DOI Creative Commons
Mária Džunková, James J. La Clair, Tomáš Tyml

et al.

Microbiome, Journal Year: 2023, Volume and Issue: 11(1)

Published: June 13, 2023

Abstract Background Nudibranchs comprise a group of > 6000 marine soft-bodied mollusk species known to use secondary metabolites (natural products) for chemical defense. The full diversity these and whether symbiotic microbes are responsible their synthesis remains unexplored. Another issue in searching undiscovered natural products is that computational analysis genomes uncultured can result detection novel biosynthetic gene clusters; however, vivo functionality not guaranteed which limits further exploration pharmaceutical or industrial potential. To overcome challenges, we used fluorescent pantetheine probe, produces CoA-analog employed biosynthesis metabolites, label capture bacterial symbionts actively producing compounds the mantle nudibranch Doriopsilla fulva. Results We recovered genome Candidatus Doriopsillibacter californiensis from Ca. Tethybacterales order, an lineage sponge found nudibranchs previously. It forms part core skin microbiome D. fulva nearly absent its internal organs. showed crude extracts contained were consistent with presence beta-lactone encoded genome. Beta-lactones represent underexplored potential have been reported Conclusions Altogether, this study shows how probe-based, targeted sorting approaches vivo.

Language: Английский

Citations

9
Divergent morphological and microbiome strategies of two neighbor sponges to cope with low pH in Mediterranean CO2 vents DOI Creative Commons

Jana Efremova,

Valerio Mazzella, Alice Mirasole

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 916, P. 170171 - 170171

Published: Jan. 19, 2024

Ocean Acidification (OA) profoundly impacts marine biochemistry, resulting in a net loss of biodiversity. Porifera are often forecasted as winner taxa, yet the strategies to cope with OA can vary and may generate diverse fitness status. In this study, microbial shifts based on V

Language: Английский

Citations

3
The Buds of Oscarella lobularis (Porifera, Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology DOI Creative Commons
Caroline Rocher,

Amélie Vernale,

Laura Fierro‐Constaín

et al.

Journal of Experimental Zoology Part B Molecular and Developmental Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

ABSTRACT The comparative study of the four non‐bilaterian phyla (Cnidaria, Placozoa, Ctenophora, and Porifera) provides insights into origin bilaterian traits. To complete our knowledge cell biology development these animals, additional models are needed. Given developmental, histological, ecological, genomic differences between sponge classes (Demospongiae, Calcarea, Homoscleromorpha, Hexactinellida), we have been developing Oscarella lobularis (Porifera, class Homoscleromorpha) model over past 15 years. Here, report a new step forward by inducing, producing, maintaining in vitro thousands clonal buds that now make possible various downstream applications. This full description bud morphology, physiology, cells tissues, from their formation to juveniles, using adapted staining protocols. In addition, show outstanding capabilities regeneration after being injured re‐epithelization dissociation. Altogether, constitute relevant all‐in‐one access large set biological processes, including somatic morphogenesis, epithelial fate, body axes formation, nutrition, contraction, ciliary beating, respiration.

Language: Английский

Citations

3