bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: June 1, 2023
Fertility-targeted
gene
drives
have
been
proposed
as
an
ethical
genetic
approach
for
managing
wild
populations
of
vertebrate
pests
public
health
and
conservation
benefit.This
manuscript
introduces
a
framework
to
identify
evaluate
target
suitability
based
on
biological
function,
expression,
results
from
mouse
knockout
models.This
identified
16
genes
essential
male
fertility
12
important
female
that
may
be
feasible
targets
mammalian
other
non-drive
pest
control
technology.
Further,
comparative
genomics
analysis
demonstrates
the
across
several
globally
significant
invasive
mammals.In
addition
providing
considerations
identifying
candidate
genes,
our
in
this
study
utility
developing
additional
tools
such
wildlife
contraceptives.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 19, 2024
Population-scale
genome
editing
can
be
used
to
alter
the
composition
or
fate
of
wild
populations.
One
approach
achieving
these
aims
utilizes
a
synthetic
gene
drive
element-a
multi-gene
cassette-to
bring
about
an
increase
in
frequency
existing
allele.
However,
use
drives
is
complicated
by
multiple
scientific,
regulatory,
and
social
issues
associated
with
transgene
persistence
flow.
Alternatives
which
transgenes
are
not
driven
could
potentially
avoid
some
issues.
Here
we
propose
population
scale
using
system
refer
as
Allele
Sail.
An
Sail
consists
editor
(the
Wind)
that
introduces
DNA
sequence
edits
Sail)
at
one
more
sites,
resulting
progeny
viable
fertile.
The
editor,
such
sequence-specific
nuclease,
prime-
base-editor,
inherited
Mendelian
fashion.
Meanwhile,
it
creates
experience
arithmetic,
Super-Mendelian
frequency.
We
explore
this
agent-based
modeling,
identify
contexts
single,
low
release
brings
very
high
also
conditions
manipulation
sex
determination
suppression.
Current
regulatory
frameworks
often
distinguish
between
transgenics
genetically
modified
organisms
(GMOs),
their
edited
non-transgenic
non-GMO.
In
context
provides
path
traits
fates
populations
ways
may
considered
acceptable.
Scientia Sinica Vitae,
Journal Year:
2022,
Volume and Issue:
52(10), P. 1522 - 1532
Published: Sept. 16, 2022
Gene
drive
refers
to
the
phenomenon
that
specific
genes
or
genetic
elements
are
passed
from
parent
offspring
in
form
of
super-Mendelian
inheritance.
In
recent
years,
based
on
characteristics
gene
and
theoretical
basis
their
molecular
mechanisms,
supported
by
CRISPR/Cas9
gene-editing
system,
gene-driven
control
technology
has
become
an
advanced
research
hotspot
field
basic
mosquito
biology
control.
And
there
have
emerged
some
practical
effective
achievements
take
into
account
ecological
stability.
This
paper
reviews
principles
drive,
CRISPR/Cas9-mediated
HDR-type
strategies,
improved
strategies
reduce
resistance
potential
risks,
simulation
analysis
drive.
It
is
hoped
provide
a
reference
for
development
system
takes
both
high
efficiency
safety
consideration.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: June 1, 2023
Fertility-targeted
gene
drives
have
been
proposed
as
an
ethical
genetic
approach
for
managing
wild
populations
of
vertebrate
pests
public
health
and
conservation
benefit.This
manuscript
introduces
a
framework
to
identify
evaluate
target
suitability
based
on
biological
function,
expression,
results
from
mouse
knockout
models.This
identified
16
genes
essential
male
fertility
12
important
female
that
may
be
feasible
targets
mammalian
other
non-drive
pest
control
technology.
Further,
comparative
genomics
analysis
demonstrates
the
across
several
globally
significant
invasive
mammals.In
addition
providing
considerations
identifying
candidate
genes,
our
in
this
study
utility
developing
additional
tools
such
wildlife
contraceptives.
