Study on the effect of 3,5,6,7,8,3′,4′-heptamethoxyflavone in Fructus Aurantii by regulating intestinal aquaporin in body fluids DOI Creative Commons

Tingting Bai,

Yating Xie,

Quan Wan

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 19, 2025

Background Fructus aurantii (FA) can cause the drying of body fluids, although specific mechanism this process remains unclear. FA is used to promote stagnation and eliminate flatulence in traditional Chinese medicine. 3,5,6,7,8,3′,4′-heptamethoxyflavone active metabolite FA, it contributes process. This paper presents an investigation into underlying mechanisms effect on fluids through regulating Aquaporin pathway. Methods Human small intestinal epithelial cells (FHs74lnt), human colon histiocytes (CCD018Co), a normal mouse, AQP3 knockout were study. Indicators included water consumption, diet, fecal content mice, as well pathological changes tissues relative expression AQP3, AQP5, AQP7, AQP11 mRNA those tissues, protein expression. ALD ADH hormone levels, upstream receptor genes AVPR1 AVPR2 also indicators for examination. Results has strong desiccating impact causing increase intake rise feces. Additionally, animal experiments have suggested connection histological damage intestine, including lymphocyte infiltration, mucosal laminae breakage, local ulceration. proteins 3, 5, 7, 11 may be regulated by 3,5,6,7,8,3′,4′-heptamethoxyflavone. increases genes, allowing them bind more easily antidiuretic aldosterone, such arginine vasopressin receptors 1 2. A number observed response conducted Aquaporin3−/− mice. The control secretion absorption thus impacted alternating 3 channels. Conclusion affect AVPR2, their binding ADH, which turn affects opening closing channel regulate body.

Language: Английский

Study on the effect of 3,5,6,7,8,3′,4′-heptamethoxyflavone in Fructus Aurantii by regulating intestinal aquaporin in body fluids DOI Creative Commons

Tingting Bai,

Yating Xie,

Quan Wan

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: May 19, 2025

Background Fructus aurantii (FA) can cause the drying of body fluids, although specific mechanism this process remains unclear. FA is used to promote stagnation and eliminate flatulence in traditional Chinese medicine. 3,5,6,7,8,3′,4′-heptamethoxyflavone active metabolite FA, it contributes process. This paper presents an investigation into underlying mechanisms effect on fluids through regulating Aquaporin pathway. Methods Human small intestinal epithelial cells (FHs74lnt), human colon histiocytes (CCD018Co), a normal mouse, AQP3 knockout were study. Indicators included water consumption, diet, fecal content mice, as well pathological changes tissues relative expression AQP3, AQP5, AQP7, AQP11 mRNA those tissues, protein expression. ALD ADH hormone levels, upstream receptor genes AVPR1 AVPR2 also indicators for examination. Results has strong desiccating impact causing increase intake rise feces. Additionally, animal experiments have suggested connection histological damage intestine, including lymphocyte infiltration, mucosal laminae breakage, local ulceration. proteins 3, 5, 7, 11 may be regulated by 3,5,6,7,8,3′,4′-heptamethoxyflavone. increases genes, allowing them bind more easily antidiuretic aldosterone, such arginine vasopressin receptors 1 2. A number observed response conducted Aquaporin3−/− mice. The control secretion absorption thus impacted alternating 3 channels. Conclusion affect AVPR2, their binding ADH, which turn affects opening closing channel regulate body.

Language: Английский

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