Prolactin Inhibition Promotes Follicle Recruitment by Increasing PIKfyve Expression in Ewes During the Estrus Stage DOI Creative Commons

Sicong Yue,

Chunhui Duan, Yong Wang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3541 - 3541

Published: Dec. 7, 2024

Prolactin (PRL) plays a key role in the growth and ovulation of animal follicles, but its impact on follicular recruitment ewes remains uncertain. In this study, total sixteen healthy (Hu sheep, aged 2-3 years, with continuous reproduction housed separately), matched for parity weight (52.98 ± 0.96 kg), were randomly assigned to two groups: control group (C) treatment (T, PRL inhibition). Ovaries collected vivo after anesthesia during estrus stage, tissue morphology was observed using hematoxylin-eosin (HE) staining. By RNA sequencing ovaries C T groups conducting bioinformatics analysis, essential genes pathways involved regulation inhibition pinpointed. Subcellular localization ovarian determined fluorescence situ hybridization (FISH) assay immunohistochemistry. The function validated knockout overexpression techniques. During phase, we noted marked rise count large follicles within following prolactin. total, 328 differentially expressed (DEGs) detected, 162 upregulated 166 downregulated. results indicated that inhibiting primarily influences follicle by acting target gene

Language: Английский

Programmed cell death in Helicobacter pylori infection and related gastric cancer DOI Creative Commons

Yukun Lin,

Kunjing Liu,

Fang Lu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: July 31, 2024

Programmed cell death (PCD) plays a crucial role in maintaining the normal structure and function of digestive tract body. Infection with Helicobacter pylori ( H. ) is an important factor leading to gastric damage, promoting Correa cascade accelerating transition from gastritis cancer. Recent research has shown that several PCD signaling pathways are abnormally activated during infection, dysfunction thought contribute development cancer interfere treatment. With deepening studies on infection terms PCD, exploring interaction mechanisms between body different may become direction for future treatment -related In addition, biologically active compounds can inhibit or induce serve as key elements this disease. review, we briefly describe process discuss cancer, summarize molecules play therapeutic each pathway process, expectation providing more comprehensive understanding infection.

Language: Английский

Citations

4
Apoptosis in polycystic ovary syndrome: Mechanisms and therapeutic implications DOI
Lan Yang,

W Ma,

Xiao Wang

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: 363, P. 123394 - 123394

Published: Jan. 13, 2025

Language: Английский

Citations

0
Fucoxanthin mitigates mercury-induced mitochondrial toxicity in the human ovarian granulosa cell line DOI Creative Commons
Ekramy Elmorsy, Huda A. Al Doghaither, Ayat B. Al‐Ghafari

et al.

Reproductive Toxicology, Journal Year: 2025, Volume and Issue: 132, P. 108855 - 108855

Published: Feb. 11, 2025

Mercury (Hg) is known to be a hazardous toxin with significant negative impact on female reproduction through mechanisms that remain unclear. The carotenoid fucoxanthin (FX) an antioxidant several positive effects human health. This study aimed examine the potential protective role of FX in reducing Hg-induced bioenergetic disturbances ovarian granulosa cell line model. (methods briefly) Hg was found reduce viability cells concentration-dependent manner, estimated 72-hour EC50 10µM. In contrast, (10 and 20µM) improved viability. (1 10µM) significantly reduced cellular ATP levels, mitochondrial membrane potential, oxygen consumption rates, lactate production. Additionally, impaired activities kinetics complexes I III expression genes ND1, ND5, cytochrome B, C oxidase, synthase subunits 6 8. According tests membranes, increased fluidity by saturated fatty acid levels increasing those unsaturated acids. also promoted swelling enhanced inner permeability hydrogen potassium ions. (10µM) shown mitigate treated cells, bioenergetics parameters, integrity manner. Based these findings, disruption may key underlying cause dysfunction. Furthermore, have therapeutic treating disorders caused bioenergetics.

Language: Английский

Citations

0
The Effect of Prolactin on Gene Expression and the Secretion of Reproductive Hormones in Ewes during the Estrus Cycle DOI Creative Commons

Sicong Yue,

Jiaxin Chen, Chunhui Duan

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(13), P. 1873 - 1873

Published: June 25, 2024

Prolactin (PRL) plays an important role in animal follicle development and ovulation. However, its regulatory effects on the different stages of estrus cycle ewes are unclear. In this study, bromocriptine (BCR, PRL inhibitor) was used to study effect secretion reproductive hormones gene expressions order explore sexual ewes. Eighty healthy with same parity similar weights were randomly assigned control group (C, n = 40) treatment (T, 40, fed bromocriptine). After synchronization, thirty-one overt signs selected from each group. Six blood samples obtained by jugular venipuncture measure concentration PRL, estrogen (E2), progesterone (P4), luteinizing hormone (LH), follicle-stimulating (FSH), gonadotropin-releasing (GnRH) proestrus, estrus, metestrus, diestrus. At time, we collected ovaries six vivo after anesthesia detect corpus luteum (CL) counts expression hormone-receptor apoptosis-related genes. The results show that inhibition had no significant length (p > 0.05). number large small follicles, levels E2, FSH, GnRH, ER, FSHR, apoptotic Caspase-3 increased < 0.05); middle follicles anti-apoptotic Bcl-2 decreased 0.05) T E2 StAR, CYP19A1, genes 0.05), LHR pro-apoptotic Bax diestrus, CL, level P4, PR, 3β-HSD, Caspase-3, summary, can affect hormones, count, during cycle. These provide a basis for understanding mechanisms underlying regulation ewe PRL.

Language: Английский

Citations

3
Prolactin Inhibition Promotes Follicle Recruitment by Increasing PIKfyve Expression in Ewes During the Estrus Stage DOI Creative Commons

Sicong Yue,

Chunhui Duan, Yong Wang

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3541 - 3541

Published: Dec. 7, 2024

Prolactin (PRL) plays a key role in the growth and ovulation of animal follicles, but its impact on follicular recruitment ewes remains uncertain. In this study, total sixteen healthy (Hu sheep, aged 2-3 years, with continuous reproduction housed separately), matched for parity weight (52.98 ± 0.96 kg), were randomly assigned to two groups: control group (C) treatment (T, PRL inhibition). Ovaries collected vivo after anesthesia during estrus stage, tissue morphology was observed using hematoxylin-eosin (HE) staining. By RNA sequencing ovaries C T groups conducting bioinformatics analysis, essential genes pathways involved regulation inhibition pinpointed. Subcellular localization ovarian determined fluorescence situ hybridization (FISH) assay immunohistochemistry. The function validated knockout overexpression techniques. During phase, we noted marked rise count large follicles within following prolactin. total, 328 differentially expressed (DEGs) detected, 162 upregulated 166 downregulated. results indicated that inhibiting primarily influences follicle by acting target gene

Language: Английский

Citations

0