Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shinji Nakatuka

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 µm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs scaffolding culturing. Results Using six types nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate exhibited swelling water retention capacities. Next, fragments examined immobilizing TKD2 mouse vascular endothelial Although most cells taken into three characterized swelling, leaked from without adhesion. solve this, comprising polyketones coated gelatin. adhesive capacity was enhanced, decreased almost all immobilized remained on sheets’ surfaces. Based these results, produced novel sheet gelatin, polyketone mixture (CPG). Since attached CPG fragment supplied little loss reached high density 3.2×109 MF-g− 1, Furthermore, continuously exosomes productivity 6–7×1010 particles ml− 1 after either 8h or 16h Conclusion CPG-based are expected wide range future applications, including exosome production

Language: Английский

Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shuji Nakatsuka

et al.

Biotechnology Letters, Journal Year: 2025, Volume and Issue: 47(2)

Published: April 1, 2025

Language: Английский

Citations

0
Exploring Exosome-Based Approaches for Early Diagnosis and Treatment of Neurodegenerative Diseases DOI
Vibhav Varshney,

Baneen C. Gabble,

Ashok Kumar Bishoyi

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 10, 2025

Language: Английский

Citations

0
Human Plasma-Derived Exosomes: A Promising Carrier System for the Delivery of Hydroxyurea to Combat Breast Cancer DOI

Waqas Khalid,

Afeefa Aslam,

Nadeem Ahmed

et al.

AAPS PharmSciTech, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 22, 2025

Language: Английский

Citations

0
Inter-Tissue Communication Mechanisms via Exosomes and Their Implications in Metabolic Diseases: Opportunities for Pharmacological Regulation DOI Creative Commons
Brenda Chimal-Vega,

Jesus Emanuel Maldonado-Arvizu,

Alicia Ávalos

et al.

Future Pharmacology, Journal Year: 2025, Volume and Issue: 5(1), P. 11 - 11

Published: March 6, 2025

Exosomes can transport regulatory biomolecules and are mediators of cellular signaling among metabolic tissues through endocrine mechanisms. Understanding the pathways processes underlying exosome-mediated inter-tissue communication is critical for elucidating molecular pathophysiology diseases such as obesity, type 2 diabetes mellitus (T2DM), cardiovascular disorders. Consequently, these mechanisms represent novel promising targets pharmacological regulation. We examined current knowledge regarding exosome physiology, interaction with target tissues, its role in tissue communication. also analyzed secretory profiles exosomes emphasizing their roles adipose tissue, liver, pancreas, skeletal muscle, small intestine, while discussing association diseases. In this sense, we propose exosomal pentad a framework highlighting inter-organ communication, where may regulate axis involving tissues. This model aligns ominous octet but emphasizes key regulators homeostasis potential therapeutic targets. The treatment emerges area pharmacologic exploration. For instance, strategies that prevent binding or expression cargo molecules miRNAs could be designed, using antagomiRs nanoparticles. Additionally, integrins like αvβ5 on membrane blocked monoclonal antibodies engineered targeted delivery molecules. Exosomes, regulation, hold to design precise molecular-level therapies minimizing systemic side effects.

Language: Английский

Citations

0
Efficient and high-density immobilization of animal cells by a microfiber with both swelling and cell adhesion properties and its application to exosome production DOI Creative Commons
Naofumi Shiomi, Pengfei Zhang,

Shinji Nakatuka

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

Abstract Purpose For high-density cell culture, we studied the development of optimal microfibers (MFs) with a 0.1–10 µm diameter, which due to their large surface area can serve as an immobilization carrier for animal cells. To date, few studies have used MFs scaffolding culturing. Results Using six types nonsoluble synthetic polymers, MF sheets were fabricated by electrospinning. The cellulose acetate, polyketone, and polyvinyl acetate exhibited swelling water retention capacities. Next, fragments examined immobilizing TKD2 mouse vascular endothelial Although most cells taken into three characterized swelling, leaked from without adhesion. solve this, comprising polyketones coated gelatin. adhesive capacity was enhanced, decreased almost all immobilized remained on sheets’ surfaces. Based these results, produced novel sheet gelatin, polyketone mixture (CPG). Since attached CPG fragment supplied little loss reached high density 3.2×109 MF-g− 1, Furthermore, continuously exosomes productivity 6–7×1010 particles ml− 1 after either 8h or 16h Conclusion CPG-based are expected wide range future applications, including exosome production

Language: Английский

Citations

0