Loss of CFTR Reverses Senescence Hallmarks in SARS-CoV-2 Infected Bronchial Epithelial Cells DOI Open Access

Flavia Merigo,

Anna Lagni, Federico Boschi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6185 - 6185

Published: June 4, 2024

SARS-CoV-2 infection has been recently shown to induce cellular senescence in vivo. A senescence-like phenotype reported cystic fibrosis (CF) models. Since the previously published data highlighted a low impact of on CFTR-defective cells, here we aimed investigate hallmarks context loss CFTR expression/function. We infected WT and KO 16HBE14o-cells with analyzed both p21 Ki67 expression using immunohistochemistry viral gene real-time PCR. Prior infection, cells displayed higher lower than cells. detected lipid accumulation identified as lipolysosomes residual bodies at subcellular/ultrastructure level. After situation reversed, high expression, well reduced Thus, activation pathways was reversed by while they were activated These uncover different response CF non-CF bronchial epithelial cell models contribute uncovering molecular mechanisms behind clinical COVID-19 patients.

Language: Английский

The Impact of Antimicrobial Resistance in Cystic Fibrosis DOI Open Access
Antonio Vitiello, Francesco Blasi, Michela Sabbatucci

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(6), P. 1711 - 1711

Published: March 16, 2024

The phenomenon of antimicrobial resistance (AMR) is a critical global health challenge, with prospects indicating its potential to become the leading cause death worldwide in coming years. Individuals pre-existing conditions, such as neoplastic disease undergoing chemotherapy, those on immunosuppressive therapy, and individuals rare diseases like cystic fibrosis (CF), face heightened challenges due AMR. CF caused by deficiency synthesis Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) channel protein, resulting multi-organ clinical symptoms, particularly respiratory system. PwCF experience recurrent pulmonary exacerbations triggered bacterial or viral infections, making them vulnerable impact This review delves into complex relationship between AMR climate dynamics, focusing unique faced CF. It discusses methods employed measure AMR, antibiotic resistance, specific microbial communities present airway. also explores intricacies within context fibrosis, emphasizing urgent need for research this field.

Language: Английский

Citations

5
The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication DOI Open Access
Paul J. Baker, Andrea C. Bohrer, Ehydel Castro

et al.

Science Immunology, Journal Year: 2024, Volume and Issue: 9(102)

Published: Dec. 6, 2024

Severity of COVID-19 is affected by multiple factors; however, it not understood how the inflammatory milieu lung at time SARS-CoV-2 exposure affects control viral replication. Here, we demonstrate that immune events in mouse closely preceding infection affect and identify innate pathways limit Pulmonary stimuli including resolved, antecedent respiratory infections with Staphylococcus aureus or influenza, ongoing pulmonary Mycobacterium tuberculosis infection, ovalbumin/alum-induced asthma, airway administration TLR ligands recombinant cytokines all establish an antiviral state restricts In addition to type I interferons, TNFα IL-1 potently precondition for enhanced control. Our work shows may benefit from immunologically quiescent microenvironment suggests heterogeneity inflammation contribute variability disease outcomes.

Language: Английский

Citations

2
The inflammatory microenvironment of the lung at the time of infection governs innate control of SARS-CoV-2 replication DOI Open Access
Paul J. Baker, Andrea C. Bohrer, Ehydel Castro

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 27, 2024

SARS-CoV-2 infection leads to vastly divergent clinical outcomes ranging from asymptomatic fatal disease. Co-morbidities, sex, age, host genetics and vaccine status are known affect disease severity. Yet, how the inflammatory milieu of lung at time exposure impacts control viral replication remains poorly understood. We demonstrate here that immune events in mouse closely preceding significantly impact we identify key innate pathways required limit replication. A diverse set pulmonary stimuli, including resolved antecedent respiratory infections with

Language: Английский

Citations

2
Occurrence of COVID-19 in cystic fibrosis patients: a review DOI Creative Commons
F Abolhasani,

Masood Moein,

Niloofar Rezaie

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 17, 2024

Cystic fibrosis (CF) is a genetic ailment caused by mutations in the cystic transmembrane conductance regulator (CFTR) gene. This autosomal recessive disorder characterized diverse pathobiological abnormalities, such as of CFTR channels mucosal surfaces, inadequate clearance mucus and sputum, addition to malfunctioning mucous organs. However, primary motive mortality CF patients pulmonary failure, which attributed colonization opportunistic microorganisms, formation resistant biofilms, subsequent decline lung characteristics. In December 2019, World Health Organization (WHO) declared outbreak radical coronavirus disease 2019 (COVID-19) worldwide public health crisis, unexpectedly spread not only within China but also globally. Given that respiration system target COVID-19 virus, it crucial investigate impact on pathogenesis patients, mainly context acute respiratory distress syndrome (ARDS). Therefore, goal this review comprehensively present literature relationship between fibrosis, contamination, development ARDS. Several investigations performed during early stages virus have discovered unexpected findings regarding occurrence effectiveness individuals with CF. Contrary initial expectancies, rate infection are lower than those overall population. finding may be different factors, including presence thick mucus, social avoidance, using remedies include azithromycin, fairly younger age decreased ACE-2 receptors, effect channel replication cycle infectivity virus. important notice certain situations, undergoing transplant, can doubtlessly boost susceptibility COVID-19. Furthermore, an increase vital take into account prevalence SARS-CoV-2 ordinary surveillance evaluate save population from capability transmission given various factors contribute precise organization.

Language: Английский

Citations

0
Loss of CFTR Reverses Senescence Hallmarks in SARS-CoV-2 Infected Bronchial Epithelial Cells DOI Open Access

Flavia Merigo,

Anna Lagni, Federico Boschi

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(11), P. 6185 - 6185

Published: June 4, 2024

SARS-CoV-2 infection has been recently shown to induce cellular senescence in vivo. A senescence-like phenotype reported cystic fibrosis (CF) models. Since the previously published data highlighted a low impact of on CFTR-defective cells, here we aimed investigate hallmarks context loss CFTR expression/function. We infected WT and KO 16HBE14o-cells with analyzed both p21 Ki67 expression using immunohistochemistry viral gene real-time PCR. Prior infection, cells displayed higher lower than cells. detected lipid accumulation identified as lipolysosomes residual bodies at subcellular/ultrastructure level. After situation reversed, high expression, well reduced Thus, activation pathways was reversed by while they were activated These uncover different response CF non-CF bronchial epithelial cell models contribute uncovering molecular mechanisms behind clinical COVID-19 patients.

Language: Английский

Citations

0