Content of Th17 related and Th2 cytokines in asthma patients with cold airway hyperresponsiveness DOI Creative Commons
А. Б. Пирогов, Anna Prikhodko, N. A. Pirogova

et al.

Medical Immunology (Russia), Journal Year: 2024, Volume and Issue: 27(2), P. 351 - 360

Published: Oct. 7, 2024

The phenomenon of cold airway hyperresponsiveness is rather common among patients with bronchial asthma. Possible participation immune mechanisms in its occurence scarcely studied. In particular, there no information about interaction between Th17-related cytokines, and cytokines Th2 response related to inflammation asthma cold-induced bronchospasm.Our objective was evaluate the contents IL-17A, IL-6, IL-22, IL-4 IL-13 interleukins patients, specify their role formation hyperresponsiveness. Spirometric indices forced expiratory flow were measured 43 content blood serum estimated before after bronchoprovocation test 3-min. isocapnic hyperventilation (-20 °C) air. Two groups formed presence (group 1, n = 14) absence 2, 29) hyperresponsiveness, verified by degree volume reduction per 1 sec. (∆FFV 1ihca ) (-16.5 (-20.0 – -12.0)% -2.3 (-3.5 -0.8)%, respectively; p < 0.0001). group when compared lower baseline values FEV (88.1±3.1% 96.6±2.2%, 0.044), midexpiratory (MEF 25-75 62.4±3.87% 75.6±3.7%, 0.013) registered. Moreover, subjects significantly higher than who did not respond There a correlation IL-17A severity reaction (∆FEV (Rs -0.33; 0.049). high IL-6 suggest both Th1/Th17 regulation bronchospasm development “Th2 low” subtype.

Language: Английский

Single-cell RNA sequencing reveals key signaling pathways and biological functions in giant cell tumors of bone DOI Creative Commons
Lin Zhong,

Zuoxi Chen

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 19, 2025

Language: Английский

Citations

0
Rocaglamide Suppresses Allergic Reactions by Regulating IL-4 Receptor Signaling DOI Creative Commons

Hyein Jo,

Mi‐Sun Kim,

Jaewhoon Jeoung

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(4), P. 840 - 840

Published: Feb. 11, 2025

Rocaglamide (Roc-A), a natural phytochemical isolated from Aglaia species, is known to exert anticancer effects. Allergic inflammation can enhance the tumorigenic potential of cancer cells. We hypothesized that Roc-A could regulate allergic inflammation. prevented an antigen increasing hallmarks reactions in vitro. suppressed passive cutaneous anaphylaxis (PCA) and systemic (PSA). RNA sequencing analysis showed expression IL-4 RBL2H3 also interleukin-4 receptor (IL-4R). was found form hydrogen-bonding network with residues N92 L64 IL-4R molecular docking simulation. inducing binding JAK1. Chromatin immunoprecipitation (ChIP) assays C-Jun bind promoter sequences IL-4R. Mouse recombinant protein increased β-hexosaminidase activity, expression, antigen-independent manner. expressions CD163 arghinase-1 markers M2 macrophages, but decreased iNOS, marker M1 macrophages lung macrophages. regulated effects culture medium antigen-stimulated cells on iNOS arginase-1 RAW264.7 The blocking or downregulation exerted negative PCA, PSA. An miR-34a mimic decreasing identified chemicals via analysis. chemical 1536801 activity reactions. effect PCA. TargetScan predicted as regulator anti-allergic its mechanisms were associated miR-34a. Taken together, our results show understanding IL-4R-mediated provide clues for development anti-allergy therapeutics.

Language: Английский

Citations

0
Unraveling heterogeneity and treatment of asthma through integrating multi-omics data DOI Creative Commons
Wei Zhang, Yu Zhang, Lifei Li

et al.

Frontiers in Allergy, Journal Year: 2024, Volume and Issue: 5

Published: Nov. 5, 2024

Asthma has become one of the most serious chronic respiratory diseases threatening people's lives worldwide. The pathogenesis asthma is complex and driven by numerous cells their interactions, which contribute to its genetic phenotypic heterogeneity. clinical characteristic insufficient for precision patient classification therapies; thus, a combination functional or pathophysiological mechanism phenotype proposes new concept called “asthma endophenotype” representing various subtypes defined distinct mechanisms. High-throughput omics approaches including genomics, epigenomics, transcriptomics, proteomics, metabolomics microbiome enable us investigate pathogenetic heterogeneity diverse endophenotypes underlying mechanisms from different angles. In this review, we provide comprehensive overview roles cell types in pathophysiology present current perspective on contribution into bidirectional interaction between airway inflammation remodeling. We next discussed how integrated analysis multi-omics data via machine learning can systematically characterize molecular biological profiles phenotype. application stratification therapies will be described. Integrating more insights key pathogenic reshape strategies management treatment.

Language: Английский

Citations

2
Deciphering the pharmacological mechanism of Radix astragali for allergic rhinitis through network pharmacology and experimental validation DOI Creative Commons
Yiwei Hua, Xi Tan, Jingwen Zhang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 2, 2024

Radix Astragali (RA) has been recognized for its therapeutic potential in allergic rhinitis (AR), yet pharmacological mechanisms remain elusive. This study systematically investigated the physicochemical properties and biological activities of RA's phytochemicals, aiming to elucidate their targets AR treatment. We identified 775 key phytochemicals intersected these with 29,544 AR-related disease targets, pinpointing 747 shared targets. A protein-protein interaction network analysis categorized into five subclusters, TNF, NFKB1, IKBKB, NFKBIA, CHUK emerging as central nodes. Enrichment revealed roles inflammatory immune responses, particularly through NF-κB, IL-17, Toll-like receptor, NOD-like receptor signaling pathways. Molecular docking dynamics simulations confirmed strong binding affinity stability In vivo, RA intervention effectively reversed expression markers an IL-13-induced nasal mucosa inflammation model. Our findings suggest that multitargeted approach involves modulation critical pathways, highlighting potential.

Language: Английский

Citations

2
Single-Cell Analysis: A Method for In-Depth Phenotyping of Cells Involved in Asthma DOI Open Access

Daniel Rodríguez-González,

Gema Guillén-Sánchez,

Victoria del Pozo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12633 - 12633

Published: Nov. 25, 2024

Asthma is a chronic inflammatory lung disease with high prevalence, making it one of the most common conditions worldwide. Its pathophysiology influenced by range genetic and environmental factors, resulting in complex heterogeneous profile. primarily associated type 2 (T2) immune response, though non-T2 endotypes also contribute to pathology. Generally, asthma characterized infiltration activation various cell types, including dendritic cells, eosinophils, innate lymphoid lymphocytes, mast neutrophils, which participate T1, T2, T17 responses. Despite advances understanding, many questions remain unresolved. Therefore, emerging omic techniques, such as single-cell RNA sequencing (scRNA-seq), offer novel insights into underlying mechanisms roles these cells. Recent scRNA-seq studies have identified multiple subtypes clusters, suggesting their potential functions The rapid advancement technology now enables in-depth investigation individual cells within tissues, allowing for precise cell-type classification detailed molecular profiling. Nonetheless, certain limitations persist, require further refinement future studies.

Language: Английский

Citations

1
Content of Th17 related and Th2 cytokines in asthma patients with cold airway hyperresponsiveness DOI Creative Commons
А. Б. Пирогов, Anna Prikhodko, N. A. Pirogova

et al.

Medical Immunology (Russia), Journal Year: 2024, Volume and Issue: 27(2), P. 351 - 360

Published: Oct. 7, 2024

The phenomenon of cold airway hyperresponsiveness is rather common among patients with bronchial asthma. Possible participation immune mechanisms in its occurence scarcely studied. In particular, there no information about interaction between Th17-related cytokines, and cytokines Th2 response related to inflammation asthma cold-induced bronchospasm.Our objective was evaluate the contents IL-17A, IL-6, IL-22, IL-4 IL-13 interleukins patients, specify their role formation hyperresponsiveness. Spirometric indices forced expiratory flow were measured 43 content blood serum estimated before after bronchoprovocation test 3-min. isocapnic hyperventilation (-20 °C) air. Two groups formed presence (group 1, n = 14) absence 2, 29) hyperresponsiveness, verified by degree volume reduction per 1 sec. (∆FFV 1ihca ) (-16.5 (-20.0 – -12.0)% -2.3 (-3.5 -0.8)%, respectively; p < 0.0001). group when compared lower baseline values FEV (88.1±3.1% 96.6±2.2%, 0.044), midexpiratory (MEF 25-75 62.4±3.87% 75.6±3.7%, 0.013) registered. Moreover, subjects significantly higher than who did not respond There a correlation IL-17A severity reaction (∆FEV (Rs -0.33; 0.049). high IL-6 suggest both Th1/Th17 regulation bronchospasm development “Th2 low” subtype.

Language: Английский

Citations

0