Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: April 24, 2025
Language: Английский
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 28, 2025
Necrotizing enterocolitis (NEC) is a critical gastrointestinal disease in preterm infants, for which no specific treatment established. We previously demonstrated that thrombin-preconditioned mesenchymal stromal cell-derived extracellular vesicles (thMSC-EVs) enhance protection against other neonatal tissue injuries. Therefore, this study aimed to evaluate the therapeutic potential of thMSC-EVs modified vitro, vivo, and organoid models NEC. In effects naïveMSC-EVs were compared hyperosmotic, ischemic, hypothermic (HIT)-stressed IEC-6 cells LPS-treated peritoneal macrophages. NEC was induced P4 mouse pups by three cycles formula feeding, oral LPS administration, hypoxia, hypothermia, followed overnight dam care. 2 × 109 intraperitoneally administered daily days, assessed macroscopically, histologically, biochemically. mouse-derived organoids established thMSC-EVs' effect mature enterocytes. LC-MS/MS performed analyze EV proteomics. with naïveMSC-EVs, significantly improved cellular viability HIT-induced reduced pro-inflammatory (IL-1α, IL-1β, TNF-α) but increased anti-inflammatory (TGF-b) cytokine levels attenuated clinical symptoms, intestinal damage, retained stem cell markers, showing more significant localization NEC-induced intestines than healthy intestines. organoids, OLFM4 claudin-4 expression stress-related markers such as sucrase-isomaltase, defensin, chromogranin A. Proteomic analysis revealed greater enriched anti-apoptotic, anti-inflammatory, adhesion, Wnt signaling pathways naïveMSC-EVs. viability, apoptosis, inflammation, upregulated key collectively suggesting their tissue-protective highlighting
Language: Английский
Citations
0
Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 4143 - 4156
Published: March 1, 2025
Cordyceps sinensis, an entomogenous fungus with unique biological properties, has demonstrated significant anti-inflammatory potential. However, its effects on inflammation regulation need to be further investigated in detail. In this study, we aimed analyze the sinensis extract (CSE) obtained via ethanol extraction and assess regulation. The secretion of pro-inflammatory cytokines (IL-6, TNF-α, IL-8, IL-1β) level MMP9, Nrf2/HO-1 ROS were evaluated. A transwell system THP-1 BEAS-2B cells was used explore inflammatory damage. Gene ontology (GO) Kyoto encyclopedia genes genomes (KEGG) enrichment analyses conducted differentially expressed genes. CSE exhibited no cytotoxicity at concentrations ≤ 1.6 mg/mL. Treatment LPS-induced significantly inhibited cytokines. reduced inflammation-related protein while upregulating signaling pathway. Fluorescence assays using DCF JC-1 confirmed that help mitigate oxidative stress-induced inflammation. treatment protected from Moreover, immune process a shared GO term between LPS-only combined LPS treatment. KEGG analysis showed is capable regulating associated responses. These findings highlight potential as immune-regulating agent functional foods health products.
Language: Английский
Citations
0
Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)
Published: April 2, 2025
Language: Английский
Citations
0
European Journal of Integrative Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 102482 - 102482
Published: April 1, 2025
Language: Английский
Citations
0
Probiotics and Antimicrobial Proteins, Journal Year: 2025, Volume and Issue: unknown
Published: April 24, 2025
Language: Английский
Citations
0