Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Feb. 6, 2024

Abstract Background Developing effective vaccines against SARS-CoV-2 that consider manufacturing limitations, equitable access, and acceptance is necessary for developing platforms to produce antigens can be efficiently presented generating neutralizing antibodies as a model new vaccines. Results This work presents the development of an applicable technology through oral administration RBD antigen fused with peptide improve its antigenic presentation. We focused on production recombinant receptor binding domain (RBD) produced in E. coli modified addition amino acids extension designed The was carried out shake flask bioreactor cultures, obtaining around 200 mg/L antigen. peptide-fused peptide-free proteins were characterized compared using SDS-PAGE gel, high-performance chromatography, circular dichroism. formulated oil-in-water emulsion mice immunization. RBD, induced robust IgG mice, capable recognizing Enzyme-linked immunosorbent assays. In addition, generated sera dosed mice. formulation showed no reactive episodes changes temperature or vomiting. Conclusions Our study demonstrated effectiveness added immunostimulation by administration. Graphical

Language: Английский

Surface displayed Porcine Epidemic Diarrhea virus membrane epitopes on Lactiplantibacillus plantarum stimulates antibody production in mice

Journal of Animal Science and Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Porcine epidemic diarrhea virus (PEDV) causes enteric disease in pigs, characterized by vomiting and watery diarrhea, has a major economic burden on the global pork industry. The objective of this study was to develop new surface display system for PEDV antigens fused with cell wall-anchoring domain, using Lactiplantibacillus plantarum as host. B-cell epitopes membrane (M) protein epitopes, designated M1, M2, M3, generated online prediction tools, were stably expressed displayed Lp. plantarum SK156 verified immunofluorescence microscopy. Stimulation porcine intestinal epithelial cells (IPEC-J2) M resulted elevated production interferon (IFN)-γ interleukin (IL)-10. To investigate immunogenicity 30 female BALB/c mice (n = 6 per group) orally administered displaying or M3 wild-type plantarum, phosphate buffered saline (PBS). On days 21 35, immunized M1 epitope showed consistently high levels antigen-specific secretory immunoglobulin (Ig)-A serum IgG, demonstrating induction both mucosal humoral immune responses. However, no changes observed cytokine profiles mice. best our knowledge, is first report lactic acid bacteria (LAB). Our findings highlight immunogenic potential possibility further research development Lactobacillus-based oral vaccine against infection.

Language: Английский