ACADEMIA JOURNAL OF BIOLOGY,
Journal Year:
2024,
Volume and Issue:
46(4), P. 71 - 89
Published: Dec. 27, 2024
Vibrio
natriegens
has
recently
been
identified
as
a
promising
host
for
the
biotechnology
industry
thanks
to
its
inherent
qualities,
which
include
fast
growth
rate,
non-pathogenicity
humans,
and
versatility
in
using
substrates.
These
advantages
have
led
potential
use
of
V.
biosynthesis
several
products.
Basically,
industrial
scale
requires
fermentation
or
cultivation
processes
be
conducted
at
high
substrate
biomass
concentrations
maximize
final
retrieved
product.
However,
studies
on
cell
density
are
limited.
Besides,
convert
recalcitrant
substrates
such
chitin
derivatives
into
biological
products
not
yet
understood.
This
review
summarizes
up-to-date
information
physiological
characteristics,
metabolism,
genome,
genetic
modification
tools
natriegens.
Subsequentially,
statistics
analysis
research
trends
related
was
presented.
Finally,
discussion
role
converting
waste
from
seafood
processing
culturing
feedstock
achieve
circular
economy
net
zero
emissions
provided.
Microbial Cell Factories,
Journal Year:
2024,
Volume and Issue:
23(1)
Published: July 24, 2024
The
diversity
of
chemical
and
structural
attributes
proteins
makes
it
inherently
difficult
to
produce
a
wide
range
in
single
recombinant
protein
production
system.
nature
the
target
themselves,
along
with
cost,
ease
use,
speed,
are
typically
cited
as
major
factors
consider
production.
Despite
variety
alternative
expression
systems,
most
for
research
therapeutics
produced
limited
number
systems:
Escherichia
coli,
yeast,
insect
cells,
mammalian
cell
lines
HEK293
CHO.
Recent
interest
Vibrio
natriegens
new
bacterial
host
is
due
part
its
short
doubling
time
≤
10
min
but
also
stems
from
promise
compatibility
techniques
genetic
systems
developed
E.
coli.
We
successfully
incorporated
V.
an
additional
system
report
improvements
published
protocols
well
that
expand
versatility
While
not
all
benefit
natriegens,
we
several
were
or
impossible
show
some
cases,
increased
yield
higher
levels
properly
folded
protein.
Additionally,
able
adapt
our
enhanced
isotope
incorporation
methods
use
natriegens.
Taken
together,
these
observations
allowed
biology,
biochemistry,
assay
development,
structure-based
drug
design
and/or
unaffordable
Microbial Cell Factories,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: March 28, 2025
Abstract
Background
Pyruvate
is
a
precursor
for
various
compounds
in
the
chemical,
drug,
and
food
industries
therefore
an
attractive
target
molecule
microbial
production
processes.
The
fast-growing
bacterium
Vibrio
natriegens
excels
with
its
specific
substrate
uptake
rate
as
unconventional
chassis
industrial
biotechnology.
Here,
we
aim
to
exploit
traits
of
V.
pyruvate
fermentations
low
biomass
concentrations.
Results
We
inactivated
dehydrogenase
complex
Δ
vnp12
,
which
harbors
deletions
prophage
regions
.
resulting
strain
aceE
was
unable
grow
minimal
medium
glucose
unless
supplemented
acetate.
In
shaking
flasks,
showed
growth
1.16
±
0.03
h
−
1
produced
4.0
0.3
g
Pyr
L
within
5
h.
optimized
parameters
aerobic
fermentation
process
applied
constant
maintenance
feed
0.24
Ac
resulted
maximal
concentration
only
6.6
0.4
CDW
yielded
highly
active
resting
cells
(q
S
)
3.5
0.2
Glc
−1
41.0
1.8
volumetric
productivity
4.1
Carbon
balancing
disclosed
gap
30%,
identified
partly
parapyruvate.
Deletion
ligK
encoding
HMG/CHA
aldolase
did
not
impact
formation
but
plasmid-based
overexpression
negatively
affected
led
3-fold
higher
parapyruvate
culture
broth.
Notably,
also
supernatants
pyruvate-producing
Corynebacterium
glutamicum
strain.
Cell-free
bioreactor
experiments
mimicking
biological
formation,
pointing
chemical
reaction
contributing
synthesis.
Conclusions
engineered
metabolically
producing
high
at
concentration.
However,
found
that
accompanied
by
well
C.
Parapyruvate
seems
be
result
conversion
might
supported
biochemically
reaction.
F1000Research,
Journal Year:
2025,
Volume and Issue:
14, P. 408 - 408
Published: April 7, 2025
Background
Nicotinamide
mononucleotide
(NMN),
is
a
promising
nutraceutical
attracting
much
attention
for
its
pharmacological
and
anti-aging
efficacies.
However,
NMN-containing
commercial
products
are
very
high-priced
due
to
the
lack
of
efficient
facile
methods
industrial-scale
production.
To
date,
various
metabolic
engineering
strategies
have
been
successfully
applied
produce
NMN
in
Escherichia
coli.
Recently,
Vibrio
natriegens
has
become
host
bioindustry
thanks
rapid
growth
capabilities
broad
substrate
utilization.
This
study
aims
evaluate
biosynthesis
capability
V.
natriegens.
Methods
Firstly,
mutant
strain
(Δdns::araC-T7RNAP-KanR
ΔpncC::FtnadE-SmR
ΔnadR)
was
generated
via
multiplex
genome
editing
by
natural
transformation
(MuGENT).
Nampt
genes
encoding
nicotinamide
phosphoribosyltransferase
from
Chitinophaga
pinensis,
Sphingopyxis
sp.
C-1,
Haemophilus
ducreyi,
Vibrio
phage
KVP40
were
codon-optimized
cloned
into
pACYCDuet™-1
under
control
T7
promoter.
The
recombinant
plasmids
electroporated
strain.
expression
NAMPTs
evaluated
SDS-PAGE
analysis
intracellular
concentrations
quantified
HPLC.
Results
After
two
rounds
MuGENT,
V54-33
generated.
demonstrated
that
all
strongly
expressed
HPLC
revealed
highest
concentration
obtained
with
NAMPT
pinensis
(44.5
μM),
followed
Vibrio
(23.3
μM).
Conclusion
feasibility
natriegens.
Bioprocess and Biosystems Engineering,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 18, 2025
Abstract
Vibrio
natriegens
is
a
fast-growing
microbial
workhorse
with
high
potential
for
biotechnological
applications.
However,
handling
the
bacterium
in
batch
processes
challenging
due
to
its
overflow
metabolism
and
mixed
acid
formation
under
microaerobic
conditions.
For
early
process
development,
technologies
enabling
small-scale
fed-batch
cultivation
of
V.
Vmax
are
needed.
In
this
study,
cultivations
96-well
microtiter
plates
were
successfully
online-monitored
first
time
µTOM
device.
Using
oxygen
transfer
rate,
scale
up
membrane-based
shake
flasks
was
performed.
The
efficiently
minimized
by
choosing
suitable
feed
rates,
prevented.
A
glucose
soft
sensor
using
rate
provided
accurate
estimates
consumption
throughout
fermentation,
eliminating
need
offline
sampling.
Analyzing
impact
inducer
IPTG
on
recombinant
production
enzyme
inulosucrase
revealed
concentration-dependent
effects
processes.
contrast,
operating
mode
resulted
activity
even
without
induction.
Overall,
an
titer
80
U/mL
achieved.
conclusion,
advantages
supported
have
been
demonstrated
development
Vmax.
Biotechnology Advances,
Journal Year:
2024,
Volume and Issue:
77, P. 108464 - 108464
Published: Oct. 9, 2024
Biotechnology
has
been
built
on
the
foundation
of
a
small
handful
well
characterized
and
well-engineered
organisms.
Recent
years
have
seen
breakout
performer
gain
attention
as
new
entrant
into
bioengineering
toolbox:
Vibrio
natriegens.
This
review
covers
recent
research
efforts
making
V.
natriegens
biotechnology
platform,
using
large
language
model
(LLM)
knowledge
graph
to
expedite
literature
survey
process.
Scientists
made
advancements
in
pertaining
fundamental
metabolic
characteristics
natriegens,
development
characterization
synthetic
biology
tools,
systems
analysis
modeling,
bioproduction
engineering,
microbial
ecology.
Each
these
subcategories
relevance
future
for
applications.
In
this
review,
we
cover
offer
context
impact
they
may
field,
highlighting
benefits
drawbacks
organism.
From
examining
research,
it
appears
that
is
precipice
becoming
platform
bacterium
biotechnology.
Critical Reviews in Biotechnology,
Journal Year:
2024,
Volume and Issue:
44(8), P. 1610 - 1637
Published: May 5, 2024
species
pose
significant
threats
worldwide,
causing
mortalities
in
aquaculture
and
infections
humans.
Global
warming
the
emergence
of
worldwide
strains
