Principles and challenges of whole cell microbial biosensors in the food industry

Journal of Food Science, Journal Year: 2024, Volume and Issue: 89(9), P. 5255 - 5269

Published: Aug. 22, 2024

Abstract Whole cell microbial biosensors (WCMB) are mostly genetically modified microorganisms used to detect target molecules as indicators of biological and chemical contaminants well in the identification compounds interest food industry. The specificity sensitivity these achieved through design genetic circuits that make use sequences such promoters, terminators, genes encoding regulatory proteins or reporter proteins, among others. Despite advances WCMBs for their application, significant challenges faced, stability, restrictions, need optimize response times so they can be a competitive detection tool market. This review explores technological progress, potential limitations industry, starting by reviewing operating principles biosensors. importance selecting appropriate chassis cells integration recognition elements transducers maximize effectiveness industry is highlighted.

Language: Английский

Recovery of organic acids from lactic acid-enriched fermentation broth via salting-out assisted solvent extraction

Process Safety and Environmental Protection, Journal Year: 2024, Volume and Issue: 187, P. 376 - 384

Published: May 3, 2024

The organic acids (OAs) market has been expanding due to their versatility and wide range of uses in several industrial applications, thus prompting a transition from chemical biological approaches generate such high-value products. Nevertheless, downstream processes separate OAs the fermentation broth represent, date, main cost manufacturing. With goal limiting this issue, present study investigated separation lactic acid (LA)-rich through physical, i.e., using ethyl acetate (EA), physico-chemical, tributyl phosphate (TBP), solvent extraction. In addition, salting-out effect different salts on recovery was investigated. Among conditions, combination pure TBP 40% ammonium sulfate ensured highest LA extraction efficiency 65%. Besides, mixing with EA (50:50) enabled maintain same while reducing process costs, toxicity chemicals involved, obtaining an extracted mix lower co-metabolites, i.e. volatile fatty acids, compared TBP. Overall, assisted by agents were shown be promising method liquid form, especially for operated at low pH.

Language: Английский

Correction: Application of whole-cell biosensors for analysis and improvement of L- and D-lactic acid fermentation by Lactobacillus spp. from the waste of glucose syrup production

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Dec. 7, 2023

Language: Английский